Glutathione -S- Transferase
(GST)
• The GST family consists of multi-functional proteins that are responsible for the detoxification and excretion
xenobiotic compounds.
• They catalyse the nucleophilic conjugation of the reduced tripeptide glutathione (GSH) thiol group to electro
substrates to convert them to less harmful and soluble compounds.
• The GST superfamily is divided into three sub-families
• soluble or cytosolic GSTs,
• mitochondrial GSTs and
• membrane-associated proteins involved in eicosanoid and glutathione metabolism.
• The GSTs are generally divided into classes based on amino acid sequence similarity, with GSTs within each c
sharing same specificity towards the electrophilic substrate and sensitivity to inhibitors.
•
• GSTs within each class typically share as little as 60 % amino acid sequence identity; however, some classes c
from as little as 40%.
, Cytosolic GST- Alpha class (1K3L)
• The GST family is also classified as member of the thioredoxin-like (Trx
superfamily.
• The members of this family are characterised by four β-sheets, sandwi
between two layers of α-helices resulting in an overall βαβ-αββα topo
termed Trx-like fold. This structural feature is present in the N-termina
domain of GST.
(GST)
• The GST family consists of multi-functional proteins that are responsible for the detoxification and excretion
xenobiotic compounds.
• They catalyse the nucleophilic conjugation of the reduced tripeptide glutathione (GSH) thiol group to electro
substrates to convert them to less harmful and soluble compounds.
• The GST superfamily is divided into three sub-families
• soluble or cytosolic GSTs,
• mitochondrial GSTs and
• membrane-associated proteins involved in eicosanoid and glutathione metabolism.
• The GSTs are generally divided into classes based on amino acid sequence similarity, with GSTs within each c
sharing same specificity towards the electrophilic substrate and sensitivity to inhibitors.
•
• GSTs within each class typically share as little as 60 % amino acid sequence identity; however, some classes c
from as little as 40%.
, Cytosolic GST- Alpha class (1K3L)
• The GST family is also classified as member of the thioredoxin-like (Trx
superfamily.
• The members of this family are characterised by four β-sheets, sandwi
between two layers of α-helices resulting in an overall βαβ-αββα topo
termed Trx-like fold. This structural feature is present in the N-termina
domain of GST.
