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BMI2601 exam answers

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BMI2601 (clinical biochemistry) exam and assignment answers










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Uploaded on
January 24, 2022
Number of pages
5
Written in
2019/2020
Type
Exam (elaborations)
Contains
Questions & answers

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Group Activity 3 [50]
Suggested Answers



Question 1 [15]

Discuss the following terms or phrases

1.1. Photoautotrophs (5)

These organisms use light as their energy source and CO 2 as their carbon source.
Photosynthetic protists and cyanobacteria employ water as the electron donor and
release oxygen. Purple sulphur bacteria and green sulphur bacteria extract electrons




m
from inorganic donors such as hydrogen, hydrogen sulphide and elemental sulphur




er as
co
because they cannot oxidize water. Photoautotrophs are important primary producers in




eH w
ecosystems




o.
1.2. Chemoorganotrophs rs e (5)
ou urc
These are organisms which use the reduced organic compounds made by autotrophs
o

as sources of energy, electrons and carbon. Chemoorganotrophs contribute to
aC s


biogeochemical cycles such as nitrogen cycle and carbon cycle in which elements are
vi y re



converted into different forms. Glucose is used as an energy source by many
chemoorganotrophs during aerobic respiration, anaerobic respiration and fermentation.
ed d




When they oxidize an organic source energy source, the electrons released are
ar stu




accepted by electron carriers such as NAD+ and FAD.
is




1.3. Substrate level phosphorylation (5)
Th




This is the synthesis of ATP from ADP by phosphorylation coupled with the
exergonic breakdown of a high-energy organic substrate molecule. The direct
sh




production of ATP during glycolysis and TCA cycle takes place by substrate level
phosphorylation. For example during glycolysis, the conversion of 1, 3
bisphosphoglycerate to 3-phosphoglycerate produces ATP by substrate level
phosphorylation.


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, Question 2 [10]

Study the Tricarboxylic acid cycle below and answer the questions which follow




m
er as
co
eH w
o.
rs e
ou urc
o
aC s
vi y re
ed d
ar stu




2.1. Carbon skeletons for amino acid biosynthesis are supplied by intermediates of the
is




citric acid cycle. Which intermediate would supply the carbon skeleton for synthesis of
Th




five-carbon amino acid? (1)
α −¿ ketoglutarate
sh




2.2. How many reduced dinucleotides would be produced with four turns of the citric
acid cycle? (2)
4 FADH2 and 12 NADH



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