Transcription Lecture 3 M2
Transcription RNAprocessing
f.EEe JetieenYtnnatiatizna
thesugar in RNA is ribose whichcarries
a hydroxyl groupon 2 carbon
DNA has deoxyribose H on 2 carbon explston
uracil lus in RNA replaces thymine t inDNA
uhas itwhere has cha
mostRNA molecules in thecellaresinglestranded DNAdoublestranded
RNAmolecules canfoldinto complex 3Dstructures foldedstructures canserve
as catalysts in biochemicalreactions
Transcription
DNAunwinds andisused as atemplate forRNA transcriptsynthesis intron noncoding sequence
ribonucleoside triphosphatesare added complementary exoncodingsequence
insequenceto theDNAtemplateaccordingtobase
pairingrules buttranscripthas uinsteadof T
RNA addsnucleotidesribonucleoside
polymerase
triphosphates in 5 3 direction likeDNA
Transcriptioninitiationandtermination
promotershortDNA nfewhundredbp whereRNApolymerase andassociatedproteinsbind to
DNA
inmanyeukaryotic andarchaealp romoters the promoterincludes a TATAbox
transcriptiontakesplace v25nucleotides downstream of TATAbox
terminator transcriptionstops attheterminator andthetranscript isreleased
proteinsinvolved
sigmafactor in bacteriaassociates w RNApolymerase
andfacilitates itsbinding w specificpromoters
generaltranscriptionfactors in eukaryotes a class of
transcriptionfactors that bindsto promoters equenceto
initiate transcription transcription requires combined
action of at least6 proteinsof generaltranscriptionfactors
Iii L'sattire ie n IncaTes sina.to
connI
transcription
qnagpygnyceye.gr fqgygqpygmnotgggngayyn.to
p othymtergangyptig
whenand in whichcellstranscriptionof a genewilloccur
mediatorcomplexof proteins RNApolymerasecolmplex
initiatestranscription enzyme that catalyzes ren that
recruitsRNA polymerase tothepromoter synthesizesRNAfromDNAtemplate in
in somemodels mediatorconnects transcription eukaryotes RNApolymerasecomplex
factors w RNApolymerase complexto responsible for transcription of protein
initiatetranscription codinggenes is called poill
RNA polymerase
RNApolymerase complextranscriptionbubbleforms transcriptiontakes
payge
complex contains structuralfeatures that separateDNAstrands allowing
trynscriptigable paired RNADNA to
region form
elongation oftranscriptoccurswithin polymerasecomplexnucleotidebynucleotide
strands Elifftriffemplate doesn'thaveproofreadingfunctionlikeDNApolymerase
at
setter
pairs
so errorrate is higherthan DNApolymerase
Transcription RNAprocessing
f.EEe JetieenYtnnatiatizna
thesugar in RNA is ribose whichcarries
a hydroxyl groupon 2 carbon
DNA has deoxyribose H on 2 carbon explston
uracil lus in RNA replaces thymine t inDNA
uhas itwhere has cha
mostRNA molecules in thecellaresinglestranded DNAdoublestranded
RNAmolecules canfoldinto complex 3Dstructures foldedstructures canserve
as catalysts in biochemicalreactions
Transcription
DNAunwinds andisused as atemplate forRNA transcriptsynthesis intron noncoding sequence
ribonucleoside triphosphatesare added complementary exoncodingsequence
insequenceto theDNAtemplateaccordingtobase
pairingrules buttranscripthas uinsteadof T
RNA addsnucleotidesribonucleoside
polymerase
triphosphates in 5 3 direction likeDNA
Transcriptioninitiationandtermination
promotershortDNA nfewhundredbp whereRNApolymerase andassociatedproteinsbind to
DNA
inmanyeukaryotic andarchaealp romoters the promoterincludes a TATAbox
transcriptiontakesplace v25nucleotides downstream of TATAbox
terminator transcriptionstops attheterminator andthetranscript isreleased
proteinsinvolved
sigmafactor in bacteriaassociates w RNApolymerase
andfacilitates itsbinding w specificpromoters
generaltranscriptionfactors in eukaryotes a class of
transcriptionfactors that bindsto promoters equenceto
initiate transcription transcription requires combined
action of at least6 proteinsof generaltranscriptionfactors
Iii L'sattire ie n IncaTes sina.to
connI
transcription
qnagpygnyceye.gr fqgygqpygmnotgggngayyn.to
p othymtergangyptig
whenand in whichcellstranscriptionof a genewilloccur
mediatorcomplexof proteins RNApolymerasecolmplex
initiatestranscription enzyme that catalyzes ren that
recruitsRNA polymerase tothepromoter synthesizesRNAfromDNAtemplate in
in somemodels mediatorconnects transcription eukaryotes RNApolymerasecomplex
factors w RNApolymerase complexto responsible for transcription of protein
initiatetranscription codinggenes is called poill
RNA polymerase
RNApolymerase complextranscriptionbubbleforms transcriptiontakes
payge
complex contains structuralfeatures that separateDNAstrands allowing
trynscriptigable paired RNADNA to
region form
elongation oftranscriptoccurswithin polymerasecomplexnucleotidebynucleotide
strands Elifftriffemplate doesn'thaveproofreadingfunctionlikeDNApolymerase
at
setter
pairs
so errorrate is higherthan DNApolymerase
