Page 1 of 4 BIOS256 OL, Week 2 Lab FINAL LABS
BIOS256
BIOS256_Labs
OL, Week
Week
2 Lab2 FINAL
lab.docx
LABS BIOS256_Labs Week 2 lab.docx.pdf
BIOS256 OL, Week 2 Lab
Name:
OL Lab 2: Intestinal Glucose Transport: Study a mouse intestine model to
diagnose an infant
Learning Objectives:
Understand the epithelial model for how glucose is transported across the mammalian
small intestine
Perform a glucose assay method
Use an animal model to study the transport of materials across the intestine
Describe the effect of sodium-potassium ATPase blocker on glucose transport by the
small intestine
Explain the effect of manipulations of mucosal concentrations of glucose and sodium on
glucose intestinal transport
Interpret physiological data and apply to clinical cases
In this simulation, you will learn how glucose absorption occurs in the intestine.
You will help treat an infant who has trouble gaining weight. She is experiencing diarrhea, with
elevated blood sodium concentration and glucose in her feces and urine. Dr. Shaw, your
supervisor, suspects this could be related to the poor glucose absorption. You will use a piece
of mouse intestine as your model to study intestinal glucose transport and see how your findings
can be used to diagnose human diseases.
Study glucose transport by measuring its concentration:
After clearing out the impurities from the everted intestinal piece, you will expose both the inner
(mucosal) and outer (serosal) sides to glucose solutions of different concentrations, a sodium-
potassium ATPase blocker, and a sodium-free saline solution.
After these treatments, you will measure the glucose levels on both sides, and analyze your
data to understand the active glucose transport through the intestinal epithelium. Also, a step-
by-step interactive summary diagram will help you visualize what happens to all the molecules
and transporters involved.
Will your findings be able to help make the infant well again?
Part 1: Complete Labster- Intestinal Glucose Transport: Study a mouse intestine
model to diagnose an infant
Part 2: Report and Reflection
This study source was downloaded by 1827175 from cliffsnotes.com on 01-08-2026 18:19:22 GMT -06:00
BIOS256 OL, Week 2 Lab FINAL LABS BIOS256_Labs Week 2 lab.docxPage 1BIOS256
of 4 OL, Week 2 Lab FINAL LABS BIOS256_Labs Week 2 lab.docx.pdf
https://www.cliffsnotes.com//study-notes/5387441
BIOS256
BIOS256_Labs
OL, Week
Week
2 Lab2 FINAL
lab.docx
LABS BIOS256_Labs Week 2 lab.docx.pdf
BIOS256 OL, Week 2 Lab
Name:
OL Lab 2: Intestinal Glucose Transport: Study a mouse intestine model to
diagnose an infant
Learning Objectives:
Understand the epithelial model for how glucose is transported across the mammalian
small intestine
Perform a glucose assay method
Use an animal model to study the transport of materials across the intestine
Describe the effect of sodium-potassium ATPase blocker on glucose transport by the
small intestine
Explain the effect of manipulations of mucosal concentrations of glucose and sodium on
glucose intestinal transport
Interpret physiological data and apply to clinical cases
In this simulation, you will learn how glucose absorption occurs in the intestine.
You will help treat an infant who has trouble gaining weight. She is experiencing diarrhea, with
elevated blood sodium concentration and glucose in her feces and urine. Dr. Shaw, your
supervisor, suspects this could be related to the poor glucose absorption. You will use a piece
of mouse intestine as your model to study intestinal glucose transport and see how your findings
can be used to diagnose human diseases.
Study glucose transport by measuring its concentration:
After clearing out the impurities from the everted intestinal piece, you will expose both the inner
(mucosal) and outer (serosal) sides to glucose solutions of different concentrations, a sodium-
potassium ATPase blocker, and a sodium-free saline solution.
After these treatments, you will measure the glucose levels on both sides, and analyze your
data to understand the active glucose transport through the intestinal epithelium. Also, a step-
by-step interactive summary diagram will help you visualize what happens to all the molecules
and transporters involved.
Will your findings be able to help make the infant well again?
Part 1: Complete Labster- Intestinal Glucose Transport: Study a mouse intestine
model to diagnose an infant
Part 2: Report and Reflection
This study source was downloaded by 1827175 from cliffsnotes.com on 01-08-2026 18:19:22 GMT -06:00
BIOS256 OL, Week 2 Lab FINAL LABS BIOS256_Labs Week 2 lab.docxPage 1BIOS256
of 4 OL, Week 2 Lab FINAL LABS BIOS256_Labs Week 2 lab.docx.pdf
https://www.cliffsnotes.com//study-notes/5387441
