UF DNA & Serology Final Exam with
complete solutions graded A+
2025/2026
______ is credited with the first practical forensic application of human DNA analysis. - correct
answer ✔✔Alec Jeffreys
RFLP is ____ sensitive as PCR methods. - correct answer ✔✔less
Which method uses the smallest fragments of DNA? - correct answer ✔✔STR
CODIS looks for relationships between the profiles of unidentified remains and relatives of
missing persons. - correct answer ✔✔True
short tandem repeats (STRs) are also known as _____ - correct answer ✔✔Microsatellites
Which is not in DAB standard 6? - correct answer ✔✔D. Amplified DNA does not need to be in
separate area than DNA extraction and PCR set-up.
When pipetting the pipet tip should be placed - correct answer ✔✔at the top of the liquid
sample
If you are LEFT handed, the order of movement would be as follows: - correct answer ✔✔Left to
right
Which is NOT appropriate during sample preparation? - correct answer ✔✔Process both
question samples and reference samples together.
,What is NOT considered a type of eye protection? - correct answer ✔✔Contacts
Which extraction method is the most appropriate for a tissue sample? - correct answer
✔✔Organic
Which extraction method is possibly too harsh for a very old, degraded bloodstain? - correct
answer ✔✔Chelex
Which extraction method is the most appropriate for a vaginal swab from a sexual assault kit? -
correct answer ✔✔Differential
Which extraction method is the most appropriate for a buccal swab (reference standard)? -
correct answer ✔✔Either Chelex or Organic
What is the main type of buffer used in the organic extraction? - correct answer ✔✔Digest
Buffer
What is the purpose of the phenol in the phenol:chloroform organic extraction? - correct
answer ✔✔Solubalize polysaccharides
The Chelex resin is selective for______? - correct answer ✔✔Heavy metals and cations
Hairs that have been shed from the head (naturally fallen) are of what category? - correct
answer ✔✔Telogen
What color blood tubes are preferred to use if DNA extraction is necessary? - correct answer
✔✔Purple
, Generally chelex extraction is used in the recovery of DNA from oral samples. However, due to
the nature of one type of sample organic extraction may be considered to provide better or
more reliable results. Which sample type is it? - correct answer ✔✔Chewing gum
True or False: The yield gel only measures total human DNA. - correct answer ✔✔False
Nucleic acids have UVVis absorption characteristics at which wavelength range? - correct
answer ✔✔240-280 nm
True or False The slot blot method of DNA quantitation can measure both double and single-
stranded DNA? - correct answer ✔✔True
RT-PCR utilizes a modification of what? - correct answer ✔✔Polymerase Chain Reaction
What type of equipment is necessary to use for RT-PCR? - correct answer ✔✔Thermal cycler
True or False? Multiplex PCR uses more than one set of PCR primers to target multiple locations.
- correct answer ✔✔True
True or False The target amount of extracted DNA necessary for PCR is in the region of 0.5 2 µg.
- correct answer ✔✔False
The short polynucleotide chains used in PCR to target regions of amplification are called? -
correct answer ✔✔Primers
In what organism is Taq Polymerase found? - correct answer ✔✔Thermus Aquaticus
What is the purpose of EDTA during the PCR reaction? - correct answer ✔✔chelator
complete solutions graded A+
2025/2026
______ is credited with the first practical forensic application of human DNA analysis. - correct
answer ✔✔Alec Jeffreys
RFLP is ____ sensitive as PCR methods. - correct answer ✔✔less
Which method uses the smallest fragments of DNA? - correct answer ✔✔STR
CODIS looks for relationships between the profiles of unidentified remains and relatives of
missing persons. - correct answer ✔✔True
short tandem repeats (STRs) are also known as _____ - correct answer ✔✔Microsatellites
Which is not in DAB standard 6? - correct answer ✔✔D. Amplified DNA does not need to be in
separate area than DNA extraction and PCR set-up.
When pipetting the pipet tip should be placed - correct answer ✔✔at the top of the liquid
sample
If you are LEFT handed, the order of movement would be as follows: - correct answer ✔✔Left to
right
Which is NOT appropriate during sample preparation? - correct answer ✔✔Process both
question samples and reference samples together.
,What is NOT considered a type of eye protection? - correct answer ✔✔Contacts
Which extraction method is the most appropriate for a tissue sample? - correct answer
✔✔Organic
Which extraction method is possibly too harsh for a very old, degraded bloodstain? - correct
answer ✔✔Chelex
Which extraction method is the most appropriate for a vaginal swab from a sexual assault kit? -
correct answer ✔✔Differential
Which extraction method is the most appropriate for a buccal swab (reference standard)? -
correct answer ✔✔Either Chelex or Organic
What is the main type of buffer used in the organic extraction? - correct answer ✔✔Digest
Buffer
What is the purpose of the phenol in the phenol:chloroform organic extraction? - correct
answer ✔✔Solubalize polysaccharides
The Chelex resin is selective for______? - correct answer ✔✔Heavy metals and cations
Hairs that have been shed from the head (naturally fallen) are of what category? - correct
answer ✔✔Telogen
What color blood tubes are preferred to use if DNA extraction is necessary? - correct answer
✔✔Purple
, Generally chelex extraction is used in the recovery of DNA from oral samples. However, due to
the nature of one type of sample organic extraction may be considered to provide better or
more reliable results. Which sample type is it? - correct answer ✔✔Chewing gum
True or False: The yield gel only measures total human DNA. - correct answer ✔✔False
Nucleic acids have UVVis absorption characteristics at which wavelength range? - correct
answer ✔✔240-280 nm
True or False The slot blot method of DNA quantitation can measure both double and single-
stranded DNA? - correct answer ✔✔True
RT-PCR utilizes a modification of what? - correct answer ✔✔Polymerase Chain Reaction
What type of equipment is necessary to use for RT-PCR? - correct answer ✔✔Thermal cycler
True or False? Multiplex PCR uses more than one set of PCR primers to target multiple locations.
- correct answer ✔✔True
True or False The target amount of extracted DNA necessary for PCR is in the region of 0.5 2 µg.
- correct answer ✔✔False
The short polynucleotide chains used in PCR to target regions of amplification are called? -
correct answer ✔✔Primers
In what organism is Taq Polymerase found? - correct answer ✔✔Thermus Aquaticus
What is the purpose of EDTA during the PCR reaction? - correct answer ✔✔chelator
