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ASCP Blood bank Questions and
Answers Latest 2026
The minimum hemoglobin concentration in a finger stick
from a male blood donor is Ans: 12.5 g/dL- all donors no
matter the sex
A cause for permanent deferral of blood donation is Ans:
History of jaundice of uncertain cause- could be a from
HBV or HCV and infection with either is a cause of
indefinite deferral
Which of the following prospective donors would be
accepted for donation Ans: A college student who's
temperature is 99.2F and states that he feels well, but is
nervous about donating- temp cannot be over 99.5F
Which one of the following constitutes permanent
rejection status of a donor Ans: A confirmed positive
HBsAg 10 years previously- a positive HBsAg at any time
is an indefinite deferral
According to AABB standards, which of the following
donors may be accepted as a blood donor Ans:
Spontaneous abortion at 2 months of pregnancy, 3
months previously- as long as she has not been pregnant
in the previous 6 weeks
Below are the results of the history obtained from a
prospective female blood donor:
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Age 16
Temp 99.0F or 37.2C
Hct 36%
History tetanus toxoid immunization 1 week previously
How many of the above results excludes this donor from
giving blood for a routine transfusion? Ans: 1-Hct must
be > 38%, temp must be <99.5F, age can be 16 unless the
law states otherwise, BP <180/100, pulse must be 50-100
unless an athlete, toxoids and vaccines from synthetic or
killed sources have no deferral
For apheresis donors who donate platelets more
frequently than every 4 weeks, a platelet count must be
performed prior to the procedure and be at least Ans:
150 x 10^3/ uL (for frequent repeat donors)- a platelet
count is not required prior to the first donation or if the
interval between donations is at least 4 weeks
Prior to blood donation, the intended venipuncture site
must be cleaned with a scrub solution containing Ans:
PVP iodine complex- if the patient is allergic to iodine
they can use a preparation of 2% chlorohexidine and 70%
isopropyl alcohol
All donor testing must include Ans: Serological test for
syphilis- First mandated donor screening test for
infectious disease
© 2025 All rights reserved
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During the preparation of platelet concentrates from
whole blood, the blood should be Ans: Cooled towards
20-24C- platelets are prepared and stored at this temp
for optimum function
The most common cause of post-trasfusion hepatitis can
be detected in donors by testing for Ans: HBsAg- the
most common post-transfusion hepatitis is HBV. HBsAg
is required for detection of acute or chronic HBV
The western blot is a confirmatory test for the presence
of Ans: anti-HIV 1
The test that is currently used to detect donors who are
infected with the AIDS virus is Ans: anti-HIV 1 & 2
A commonly used screening method for anti-HIV-1
detection is Ans: ELISA
Rejuvenation of a unit of RBC is a method used to Ans:
restore 2,3-DPG and ATP to normal levels
A unit of packed cells is split into 2 aliquots under closed
sterile conditions at 8 AM. The expiration time for each
aliquot is now Ans: The original date of the unsplit unit-
sterile docking devices allow entry into donor units
without affecting the expiration date of the product
A unit of RBC expiring in 35 days is split into 5 small
aliquots using a sterile pediatric quad set and a sterile
connecting device. Each aliquot must be labeled as
expiring in Ans: 35 days- Sterile docking devices allow
© 2025 All rights reserved
ASCP Blood bank Questions and
Answers Latest 2026
The minimum hemoglobin concentration in a finger stick
from a male blood donor is Ans: 12.5 g/dL- all donors no
matter the sex
A cause for permanent deferral of blood donation is Ans:
History of jaundice of uncertain cause- could be a from
HBV or HCV and infection with either is a cause of
indefinite deferral
Which of the following prospective donors would be
accepted for donation Ans: A college student who's
temperature is 99.2F and states that he feels well, but is
nervous about donating- temp cannot be over 99.5F
Which one of the following constitutes permanent
rejection status of a donor Ans: A confirmed positive
HBsAg 10 years previously- a positive HBsAg at any time
is an indefinite deferral
According to AABB standards, which of the following
donors may be accepted as a blood donor Ans:
Spontaneous abortion at 2 months of pregnancy, 3
months previously- as long as she has not been pregnant
in the previous 6 weeks
Below are the results of the history obtained from a
prospective female blood donor:
© 2025 All rights reserved
, 2 | Page
Age 16
Temp 99.0F or 37.2C
Hct 36%
History tetanus toxoid immunization 1 week previously
How many of the above results excludes this donor from
giving blood for a routine transfusion? Ans: 1-Hct must
be > 38%, temp must be <99.5F, age can be 16 unless the
law states otherwise, BP <180/100, pulse must be 50-100
unless an athlete, toxoids and vaccines from synthetic or
killed sources have no deferral
For apheresis donors who donate platelets more
frequently than every 4 weeks, a platelet count must be
performed prior to the procedure and be at least Ans:
150 x 10^3/ uL (for frequent repeat donors)- a platelet
count is not required prior to the first donation or if the
interval between donations is at least 4 weeks
Prior to blood donation, the intended venipuncture site
must be cleaned with a scrub solution containing Ans:
PVP iodine complex- if the patient is allergic to iodine
they can use a preparation of 2% chlorohexidine and 70%
isopropyl alcohol
All donor testing must include Ans: Serological test for
syphilis- First mandated donor screening test for
infectious disease
© 2025 All rights reserved
, 3 | Page
During the preparation of platelet concentrates from
whole blood, the blood should be Ans: Cooled towards
20-24C- platelets are prepared and stored at this temp
for optimum function
The most common cause of post-trasfusion hepatitis can
be detected in donors by testing for Ans: HBsAg- the
most common post-transfusion hepatitis is HBV. HBsAg
is required for detection of acute or chronic HBV
The western blot is a confirmatory test for the presence
of Ans: anti-HIV 1
The test that is currently used to detect donors who are
infected with the AIDS virus is Ans: anti-HIV 1 & 2
A commonly used screening method for anti-HIV-1
detection is Ans: ELISA
Rejuvenation of a unit of RBC is a method used to Ans:
restore 2,3-DPG and ATP to normal levels
A unit of packed cells is split into 2 aliquots under closed
sterile conditions at 8 AM. The expiration time for each
aliquot is now Ans: The original date of the unsplit unit-
sterile docking devices allow entry into donor units
without affecting the expiration date of the product
A unit of RBC expiring in 35 days is split into 5 small
aliquots using a sterile pediatric quad set and a sterile
connecting device. Each aliquot must be labeled as
expiring in Ans: 35 days- Sterile docking devices allow
© 2025 All rights reserved
