Plasmid Study guides, Class notes & Summaries

Describe the events of fertilisation that occur after the acrosome reaction. -Membrane of sperm and egg cell fuse together -cortical granules release contents into zona pellucida -Zona pellucida thickens -Haploid nuclei fuse Explain how phospholipids form a cell surface membrane -Hydrophilic parts associate with water -Hydrophobic parts repel water -a bilayer forms with hydrophobic parts pointing in towards the centre of the bilayer Describe the role of the CFTR protein in e...

1. Say you have a 1.0 mg/ml solution and some rando gives you 100 micro- liters of the solution to your 100 microliters. What is the dilution ratio?: 1:2 2. What does step mean in serial dilutions?: Number of times you do the fold 3. What does fold mean in serial dilution?: Factor by which you are diluting, Ex: 1/2 4. How many grams in a Kg: 1000 grams 5. How many minimoles (mM) in a mole?: 1000 minimoles 6. How many mL in a L?: 1000 mL 7. Should the concentration (independent variable) be...

OpenStax Microbiology Test Bank Chapter 12: Modern Applications of Microbial Genetics Chapter 12: Modern Applications of Microbial Genetics * = Correct answer Multiple Choice 1. Which of the following is the definition of biotechnology? A. The alteration of DNA in a cell B. The use of living systems to benefit humankind* C. Using computers to synthesize biomolecules D. Using DNA for solving mathematical problems Difficulty: Easy ASM Standard: 26 2. Which of the following is the defin...

S. marcescens is gram _____ Negative What is used to generate S. marcescens strains with mutations in pigment-generating genes transposon mutagenesis What is a transposon? a mobile genetic element. A transposon contains one or more genes, including a transposase gene, which are flanked by insertion sequences What is a transposase? an enzyme that facilitates the genetic element's movement about the genome How is a transposon different from a plasmid? Unlike a pla...

how much BSA is needed to prepare 50 mL of 5 mg/ml of BSA (w/v)? (50 mL)(5 mg,mL) = 250 mg a technician is assigned the task of inserting a gene, LanA, into a plasmid to ultimately be expressed as a therapeutic protein in E coli. label the following steps (1-4) in the correct order the technician must perform them to accomplish her task. 1) design primers to amplify the lanA sequence using polymerase chain reaction (PCR) and perform the PCR reaction 2) digest and provide lanA PCR pro...

What is the purpose of having rrnBT4 in an expression vector? - correct answerEnhances the stability of the GOI transcript Why must reverse transcriptase be used to create a eukaryotic expression library? - correct answerThe resulting cDNAs do not contain non-protein coding introns Which type of ligation-independent cloning technique takes advantage of the recombination between lambda phage attB and attP sequences in the vectors and in the DNA fragment to be inserted? - correct answerGatew...

how much BSA is needed to prepare 50 mL of 5 mg/ml of BSA (w/v)? - Answer- (50 mL)(5 mg,mL) = 250 mg a technician is assigned the task of inserting a gene, LanA, into a plasmid to ultimately be expressed as a therapeutic protein in E coli. label the following steps (1-4) in the correct order the technician must perform them to accomplish her task. - Answer- 1) design primers to amplify the lanA sequence using polymerase chain reaction (PCR) and perform the PCR reaction 2) digest and provid...

Fundamentals of Biochemistry Life at the Molecular Level 4th edition by Voet- Test Bank Sample Test Chapter 3: Nucleotides, Nucleic Acids, and Genetic Information Matching A) diploid B) phosphodiester C) probe D) pyrimidine E) introns F) cloning G) transformed H) exons I) ampicillin J) autoradiography K) chain-terminator L) purine M) ester 1. Cytosine, uracil, and thymine are derivatives of . Ans: ...

After successful bacterial transformation with plasmid dna, you plan to isolate the plasmid dna from the genomic dna using a miniprep. What should you not do? - answer- use water to precipitate and wash dna How would you describe a tertiary protein structure? - answer- the three dimensional shape of the polypeptide chain once is folded What is true about about protein synthesis? - answer- transfer rna (trna) molecules that carry amino acids You observe an unknown cell under a microscope...

Medical Interventions Final Exam With All solutions complete Antibody Antigen Bioinformatics Concentration ELISA (Enzyme-linked Immunosorbant Assay) Enzyme Genome Medical Intervention Outbreak pathogen primer) serial dilution solute solution solvent substrate antibiotic antibiotic resistance conjugation nucleoid plasmid transduction transformation audiogram cochlear

what represents the correct order of assembly in the cell Amino acid, polypeptide, protein t/f: A single base pair change in a gene's DNA sequence can affect the shape and function of the protein it encodes. true t/f:The three-dimensional structure of a protein is largely determined by the amino acid sequence. true A structural gene's code is 5' TAC ATG CCC TTA ATC 3', what will the mRNA transcript be? 3' AUG UAC GGG AAU UAG 5' Circular, long DNA containing ...