FACS EXAM QUESTIONS WITH CORRECT ANSWERS LATEST UPDATE 2025/2026
What kind of cellular properties can be used to sort a population of cells using FACs? - Answers
All of them
What is the flow buffer made of? - Answers Saline solutions
Why is pressure so important in FACs? - Answers To move both the sample and the flow buffer
through the system
What is the importance of the flow cell? - Answers To create a uniform stream for the samples
in flow buffer
What is the function of the closed-loop nozzle? - Answers Ensure a good fluidic system start up
and shut down procedure
How can you measure the cell size? - Answers Using FSC values
Which of these components is not part of the optics system? - Answers The nozzle
What is the interrogation point? - Answers Where the cells intercept the laser beam trajectory
What is the droplet break-off point? - Answers It's the point on the stream where it begins to be
partitioned into droplets
Why is it so important to set a good drop delay in FACS? - Answers It allows the system to
decide when to apply the electric charge to the droplets
What is the number of events per second? - Answers The number of cells per second that are
being loaded into the fluidics system
Why is it important to set the voltage for FSC and SSC before sorting cells? - Answers To ensure
that the system detects all the cells in the sorting process
How do you think the fluorescence signal should appear in the positive control? - Answers Very
high, there would be a peak on the right side of the x-axis
How does gating help sort cells? - Answers It sets the charges that should be applied to the
droplets in order to sort cells
What would happen to those cells outside of the gating requirements? - Answers They will be
discarted
What is the purpose of the optical system? - Answers To excite the sample and collect in the
emitted light signals
Which of these statements related to different FACS parameters is false? - Answers The drop
delay is the distance between the sample and the droplet break-off
What kind of cellular properties can be used to sort a population of cells using FACs? - Answers
All of them
What is the flow buffer made of? - Answers Saline solutions
Why is pressure so important in FACs? - Answers To move both the sample and the flow buffer
through the system
What is the importance of the flow cell? - Answers To create a uniform stream for the samples
in flow buffer
What is the function of the closed-loop nozzle? - Answers Ensure a good fluidic system start up
and shut down procedure
How can you measure the cell size? - Answers Using FSC values
Which of these components is not part of the optics system? - Answers The nozzle
What is the interrogation point? - Answers Where the cells intercept the laser beam trajectory
What is the droplet break-off point? - Answers It's the point on the stream where it begins to be
partitioned into droplets
Why is it so important to set a good drop delay in FACS? - Answers It allows the system to
decide when to apply the electric charge to the droplets
What is the number of events per second? - Answers The number of cells per second that are
being loaded into the fluidics system
Why is it important to set the voltage for FSC and SSC before sorting cells? - Answers To ensure
that the system detects all the cells in the sorting process
How do you think the fluorescence signal should appear in the positive control? - Answers Very
high, there would be a peak on the right side of the x-axis
How does gating help sort cells? - Answers It sets the charges that should be applied to the
droplets in order to sort cells
What would happen to those cells outside of the gating requirements? - Answers They will be
discarted
What is the purpose of the optical system? - Answers To excite the sample and collect in the
emitted light signals
Which of these statements related to different FACS parameters is false? - Answers The drop
delay is the distance between the sample and the droplet break-off
