NHA Phlebotomy Exam Questions and
Answers Graded A+
First in the order of draw. Used to check for infection in the bloodstream. Do not
invert or spin. - Correct answer-First in the order of draw. Used to check for
infection in the bloodstream. Do not invert or spin.
Light blue top tube - Correct answer-Second in the order of draw. Sodium Citrate
tube, invert 3-4 times. The tube must be filled fully. When using a butterfly needle,
a discard tube must be used before fully filling a light blue-topped tube. (used for
coagulation tests.
Gold topped tube - Correct answer-Third in the order of draw. Is a Gel, or a Serum
tube. Do not invert, or spin. Do not use gel tubes for toxicology or drug testing.
Red or Black topped tube - Correct answer-Fourth in the order of draw. Serum tube
with no Gel, or SST with gel. Invert 5 times, allow to clot for 30 minutes, then spin
for 10-15 minutes. Then pour into a transport vial labeled with specimen type and
patient ID.
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, Green or Tan topped tube - Correct answer-Fifth in the order of draw. Heparin tube.
Needs to be inverted 8-10 times. Do not spin.
Lavender or Tan topped tube - Correct answer-Sixth in the order of draw. EDTA
tube. Invert 8-10 times. Do not Spin.
Royal Blue tube - Correct answer-Seventh in the order of draw. EDTA tube. Do not
invert, do not spin. It can occasionally be used the same as a red-topped tube.
Grey tube - Correct answer-Eighth in the order of draw. Sodium Fluoride
(Glucose) or Glycolic inhibitor. Invert 8-10 times. Do not spin.
Yellow topped tubes - Correct answer-Ninth in the order of draw, this tube is to be
drawn last. Citrate ACD. Do not invert.
Serum Iron test - Correct answer-Used to measure iron in serum to check iron
levels. Red topped or tiger topped SST tubes
TBIC (total iron binding capacity) test - Correct answer-Measures bloods ability to
bind iron with transferrin. Tested in red top or tiger topped SST tubes
Ferritin test - Correct answer-Measures iron storage in the body. Red topped or
tiger topped SST tubed
Reticulocyte count - Correct answer-Evaluates red blood cell production. Tested in
a lavender top EDTA tube.
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Answers Graded A+
First in the order of draw. Used to check for infection in the bloodstream. Do not
invert or spin. - Correct answer-First in the order of draw. Used to check for
infection in the bloodstream. Do not invert or spin.
Light blue top tube - Correct answer-Second in the order of draw. Sodium Citrate
tube, invert 3-4 times. The tube must be filled fully. When using a butterfly needle,
a discard tube must be used before fully filling a light blue-topped tube. (used for
coagulation tests.
Gold topped tube - Correct answer-Third in the order of draw. Is a Gel, or a Serum
tube. Do not invert, or spin. Do not use gel tubes for toxicology or drug testing.
Red or Black topped tube - Correct answer-Fourth in the order of draw. Serum tube
with no Gel, or SST with gel. Invert 5 times, allow to clot for 30 minutes, then spin
for 10-15 minutes. Then pour into a transport vial labeled with specimen type and
patient ID.
©COPYRIGHT 2025, ALL RIGHTS RESERVED 1
, Green or Tan topped tube - Correct answer-Fifth in the order of draw. Heparin tube.
Needs to be inverted 8-10 times. Do not spin.
Lavender or Tan topped tube - Correct answer-Sixth in the order of draw. EDTA
tube. Invert 8-10 times. Do not Spin.
Royal Blue tube - Correct answer-Seventh in the order of draw. EDTA tube. Do not
invert, do not spin. It can occasionally be used the same as a red-topped tube.
Grey tube - Correct answer-Eighth in the order of draw. Sodium Fluoride
(Glucose) or Glycolic inhibitor. Invert 8-10 times. Do not spin.
Yellow topped tubes - Correct answer-Ninth in the order of draw, this tube is to be
drawn last. Citrate ACD. Do not invert.
Serum Iron test - Correct answer-Used to measure iron in serum to check iron
levels. Red topped or tiger topped SST tubes
TBIC (total iron binding capacity) test - Correct answer-Measures bloods ability to
bind iron with transferrin. Tested in red top or tiger topped SST tubes
Ferritin test - Correct answer-Measures iron storage in the body. Red topped or
tiger topped SST tubed
Reticulocyte count - Correct answer-Evaluates red blood cell production. Tested in
a lavender top EDTA tube.
©COPYRIGHT 2025, ALL RIGHTS RESERVED 2
