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MCB 250 EXAM 3 | QUESTIONS AND ANSWERS | 2025/2026 UPDATE | 100% CORRECT

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MCB 250 EXAM 3 | QUESTIONS AND ANSWERS | 2025/2026 UPDATE | 100% CORRECT

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MCB 250 EXAM 3 | QUESTIONS AND ANSWERS |
2025/2026 UPDATE | 100% CORRECT




Homologous recombination involves all of the following except ______.
A. a double stranded break in the DNA
B. formation of a heteroduplex sequence
C. two nearly identical DNA sequences
D. alignment of DNA on different chromosomes
E. addition of Uracil into the DNA Answer - E. addition of Uracil into the DNA


In homologous recombination, the term "patch product" refers to _____.
A. a localized change in DNA sequence resulting from DNA mismatch repair.
B.the junction site of chromosomal crossing over.
C. the product of any Holliday junction resolution.
D. short stretches of DNA cut out by the Uvr system.
E. a region of partially newly synthesized DNA flanked on either side by
sequences that have not experienced re-assortment Answer - E. a region of
partially newly synthesized DNA flanked on either side by sequences that have
not experienced re-assortment


In E. coli, which protein is central to the process of strand invasion?
A. RecD
B. RecA
C. RecC
D. RecUV

, E. RecB Answer - B. RecA


The biggest difference between homologous recombination and non-
homologous end joining is:
A. The need to process the ends of the DNA
B. The ability to restore the DNA to its original sequence
C. The need to ligate the strands to complete the repair
D. Non-homologous end joining is found only in bacteria Answer - B. The
ability to restore the DNA to its original sequence


CRISPR can be used to edit a eukaryotic chromosome. It can be used to mutate
a wild type sequence or repair a defect back to wildtype. In order to carry out
repair, in addition to the CRISPR proteins and guide RNA, one needs to add
which of following to the cells?
A. Ligase
B. RecA protein
C. Mediator
D. DNA with the wild type sequence Answer - D. DNA with the wild type
sequence


In a monohybrid cross, what is the probability of seeing the recessive
phenotype in the F2 progeny?
A. 3/4
B. 1/4
C. 2/3
D. 1/3 Answer - B. 1/4


Why do alleles of genes on the same chromosome sort independently during
meiosis?

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