MB ASCP EXAM LATEST 2023-2024 ACTUAL EXAM 200 QUESTIONS AND CORRECT ANSWERS
Predominant form of DNA - (answer) Right-handed B
DNA and histone interaction is due to what type of bond? - (answer) Ionic
What stains to the minor groove of DNA duplex? - (answer) SYBR Green
What is the most abundant type of RNA? - (answer) Ribosomal RNA (rRNA)
What does RNA act as in a retrovirus? - (answer) Genome and mRNA
What increases half-life of mRNA? - (answer) 5' Methyl Cap
What kind of DNA is most likely to be transcriptionally active? - (answer) Euchromatin
What is the most common type of eukaryotic DNA? - (answer) Satellite DNA
What specimen has the most DNA according to molecular weight? - (answer) White Blood Cells
What amino acids compose histones? - (answer) Lysine and Arginine
Amino acid that all proteins start with: - (answer) Methionine
What protects the DNA strand during replication? - (answer) Single-stranded binding protein
Leading Strand - (answer) 3' to 5' template
Lagging Strand - (answer) 5' to 3' template
What DNA strand is being copied in transcription? - (answer) Antisense Strand
,MB ASCP EXAM LATEST 2023-2024 ACTUAL EXAM 200 QUESTIONS AND CORRECT ANSWERS
First step after newly formed mRNA is made? - (answer) Addition of poly(A) tail
Every time the chromosome is copied it would become shorter if not for what enzyme? - (answer)
Telomerase
What polymerase has repair activity? - (answer) Beta/Epsilon
What polymerase acts on leading strand? - (answer) Delta
What polymerase acts on lagging strand? - (answer) Alpha
Which polymerase is used for mitochondrial replication and repair? - (answer) Gamma
What polymerase binds the 5' end to 3' end of dsDNA? - (answer) Topoisomerase
Which RNA polymerase is mainly for transcription? - (answer) RNA Pol II
Which polymerase is involved with mRNA synthesis? - (answer) RNA Pol II
What inactivates RNAses? - (answer) Diethyl pyrocarbonate (DEPC) or bake glassware for 4 to 6 hours
at 400C
What type of restriction enzyme recognizes palindromic sites? - (answer) Restriction Enzyme Type II
What type of restriction enzyme lacks methylation activity? - (answer) Restriction Enzyme Type II
What is necessary to make cDNA? - (answer) Reverse transcriptase
, MB ASCP EXAM LATEST 2023-2024 ACTUAL EXAM 200 QUESTIONS AND CORRECT ANSWERS
Number of SNPs in a human genome? - (answer) 11 billion (10^7)
How many genes (base pairs) are in a human haploid genome? - (answer) 3 billion base pairs
The polymerization of acrylamide requires: - (answer) Catalyst (APS or TEMED) or Light activation
How does agarose gel pore size relate to concentration? - (answer) Inversely proportional
What is denatured during a southern blot? - (answer) Hydrogen bonds (inter-strand bonds)
Melting temperature formula - (answer) Tm = (4C x GC Pairs) + (2C x AT pairs)
Which is likely to interfere with fluorescent probe? - (answer) 5' G
Proper GC content of primer - (answer) 35-65%
Inhibitors of PCR - (answer) Hemoglobin, Heparin, Xylene, Phenol, Cyanol, and Bromophenol Blue
Order to add to a PCR reaction: - (answer) Master mix > patient sample > positive control > negative
control
Pre Area - (answer) Positive Pressure
Post Area - (answer) Negative Pressure
Which PCR requires two steps for specificity? - (answer) Nested PCR
Which microorganism is detected by branched DNA amplification? - (answer) DNA or RNA
Predominant form of DNA - (answer) Right-handed B
DNA and histone interaction is due to what type of bond? - (answer) Ionic
What stains to the minor groove of DNA duplex? - (answer) SYBR Green
What is the most abundant type of RNA? - (answer) Ribosomal RNA (rRNA)
What does RNA act as in a retrovirus? - (answer) Genome and mRNA
What increases half-life of mRNA? - (answer) 5' Methyl Cap
What kind of DNA is most likely to be transcriptionally active? - (answer) Euchromatin
What is the most common type of eukaryotic DNA? - (answer) Satellite DNA
What specimen has the most DNA according to molecular weight? - (answer) White Blood Cells
What amino acids compose histones? - (answer) Lysine and Arginine
Amino acid that all proteins start with: - (answer) Methionine
What protects the DNA strand during replication? - (answer) Single-stranded binding protein
Leading Strand - (answer) 3' to 5' template
Lagging Strand - (answer) 5' to 3' template
What DNA strand is being copied in transcription? - (answer) Antisense Strand
,MB ASCP EXAM LATEST 2023-2024 ACTUAL EXAM 200 QUESTIONS AND CORRECT ANSWERS
First step after newly formed mRNA is made? - (answer) Addition of poly(A) tail
Every time the chromosome is copied it would become shorter if not for what enzyme? - (answer)
Telomerase
What polymerase has repair activity? - (answer) Beta/Epsilon
What polymerase acts on leading strand? - (answer) Delta
What polymerase acts on lagging strand? - (answer) Alpha
Which polymerase is used for mitochondrial replication and repair? - (answer) Gamma
What polymerase binds the 5' end to 3' end of dsDNA? - (answer) Topoisomerase
Which RNA polymerase is mainly for transcription? - (answer) RNA Pol II
Which polymerase is involved with mRNA synthesis? - (answer) RNA Pol II
What inactivates RNAses? - (answer) Diethyl pyrocarbonate (DEPC) or bake glassware for 4 to 6 hours
at 400C
What type of restriction enzyme recognizes palindromic sites? - (answer) Restriction Enzyme Type II
What type of restriction enzyme lacks methylation activity? - (answer) Restriction Enzyme Type II
What is necessary to make cDNA? - (answer) Reverse transcriptase
, MB ASCP EXAM LATEST 2023-2024 ACTUAL EXAM 200 QUESTIONS AND CORRECT ANSWERS
Number of SNPs in a human genome? - (answer) 11 billion (10^7)
How many genes (base pairs) are in a human haploid genome? - (answer) 3 billion base pairs
The polymerization of acrylamide requires: - (answer) Catalyst (APS or TEMED) or Light activation
How does agarose gel pore size relate to concentration? - (answer) Inversely proportional
What is denatured during a southern blot? - (answer) Hydrogen bonds (inter-strand bonds)
Melting temperature formula - (answer) Tm = (4C x GC Pairs) + (2C x AT pairs)
Which is likely to interfere with fluorescent probe? - (answer) 5' G
Proper GC content of primer - (answer) 35-65%
Inhibitors of PCR - (answer) Hemoglobin, Heparin, Xylene, Phenol, Cyanol, and Bromophenol Blue
Order to add to a PCR reaction: - (answer) Master mix > patient sample > positive control > negative
control
Pre Area - (answer) Positive Pressure
Post Area - (answer) Negative Pressure
Which PCR requires two steps for specificity? - (answer) Nested PCR
Which microorganism is detected by branched DNA amplification? - (answer) DNA or RNA
