Please check the examination details below before entering your candidate information
Candidate surname Other names
Centre Number Candidate Number
Pearson Edexcel Level 3 GCE
Thursday 5 June 2025
Afternoon (Time: 1 hour 45 minutes) Paper
reference 9BI0/01
Biology B
Advanced
PAPER 1: Advanced Biochemistry, Microbiology
and Genetics
You must have: Total Marks
Scientific calculator, HB pencil, ruler
Instructions
•• Use black ink or ball-point pen.
Fill in the boxes at the top of this page with your name,
centre number and candidate number.
•• Answer all questions.
Answer the questions in the spaces provided
– there may be more space than you need.
Information
•• The total mark for this paper is 90.
The marks for each question are shown in brackets
– use this as a guide as to how much time to spend on each question.
• toIn structure
question(s) marked with an asterisk (*), marks will be awarded for your ability
your answer logically showing how the points that you make are
related or follow on from each other where appropriate.
Advice
•• Read each question carefully before you start to answer it.
Try to answer every question.
• Check your answers if you have time at the end.
Turn over
P74459A
©2025 Pearson Education Ltd.
Y:1/1/1/1/1/1/1/1/
*P74459A0136*
, Answer ALL questions.
Some questions must be answered with a cross in a box . If you change your mind about an
DO NOT WRITE IN THIS AREA DO NOT WRITE IN THIS AREA DO NOT WRITE IN THIS AREA
answer, put a line through the box and then mark your new answer with a cross .
1 Active transport is one type of cell transport mechanism.
(a) What happens during active transport?
(1)
A ATP is hydrolysed releasing energy
B ATP is hydrolysed using energy
C ATP is phosphorylated releasing energy
D ATP is phosphorylated using energy
(b) Which substance is transported against a concentration gradient by active
transport?
(1)
A hydrophilic molecule
B large hydrophobic molecule
C small hydrophobic molecule
D water
2
*P74459A0236*
, (c) The graph shows the change in concentration of molecule A and molecule B, as
they are transported into the cytoplasm of a cell.
DO NOT WRITE IN THIS AREA DO NOT WRITE IN THIS AREA DO NOT WRITE IN THIS AREA
molecule A
Concentration of molecules
in the cytoplasm molecule B
0 10 20 30
Time / mins
Explain the differences in the changes in concentration of molecule A and
molecule B between 0 and 10 minutes and between 20 and 30 minutes.
(2)
. . . . . . . . . . . .. .. .. .. .. .. .. .. .. .. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . ............................................................................................................................................ ................ . . . . . . . . . . . . . . . . . . . .
. . . . . . . . . . . .. .. .. .. .. .. .. .. .. .. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . ............................................................................................................................................ ................ . . . . . . . . . . . . . . . . . . . .
. . . . . . . . . . . .. .. .. .. .. .. .. .. .. .. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . ............................................................................................................................................ ................ . . . . . . . . . . . . . . . . . . . .
. . . . . . . . . . . .. .. .. .. .. .. .. .. .. .. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . ............................................................................................................................................ ................ . . . . . . . . . . . . . . . . . . . .
. . . . . . . . . . . .. .. .. .. .. .. .. .. .. .. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . ............................................................................................................................................ ................ . . . . . . . . . . . . . . . . . . . .
. . . . . . . . . . . .. .. .. .. .. .. .. .. .. .. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . ............................................................................................................................................ ................ . . . . . . . . . . . . . . . . . . . .
(Total for Question 1 = 4 marks)
3
*P74459A0336* Turn over
, 2 The diagram shows part of cellular respiration.
glucose
DO NOT WRITE IN THIS AREA DO NOT WRITE IN THIS AREA DO NOT WRITE IN THIS AREA
glycolysis
molecule X
link reaction
and
Krebs cycle
carbon dioxide
(a) Which is molecule X?
(1)
A glycerate 3-phosphate
B glycogen
C lactate
D pyruvate
(b) Which row of the table shows the locations of glycolysis, the link reaction and the
Krebs cycle in eukaryotic cells?
(1)
Glycolysis Link reaction Krebs cycle
A cytoplasm cytoplasm cytoplasm
B cytoplasm mitochondria mitochondria
C mitochondria cytoplasm cytoplasm
D mitochondria mitochondria mitochondria
4
*P74459A0436*
Candidate surname Other names
Centre Number Candidate Number
Pearson Edexcel Level 3 GCE
Thursday 5 June 2025
Afternoon (Time: 1 hour 45 minutes) Paper
reference 9BI0/01
Biology B
Advanced
PAPER 1: Advanced Biochemistry, Microbiology
and Genetics
You must have: Total Marks
Scientific calculator, HB pencil, ruler
Instructions
•• Use black ink or ball-point pen.
Fill in the boxes at the top of this page with your name,
centre number and candidate number.
•• Answer all questions.
Answer the questions in the spaces provided
– there may be more space than you need.
Information
•• The total mark for this paper is 90.
The marks for each question are shown in brackets
– use this as a guide as to how much time to spend on each question.
• toIn structure
question(s) marked with an asterisk (*), marks will be awarded for your ability
your answer logically showing how the points that you make are
related or follow on from each other where appropriate.
Advice
•• Read each question carefully before you start to answer it.
Try to answer every question.
• Check your answers if you have time at the end.
Turn over
P74459A
©2025 Pearson Education Ltd.
Y:1/1/1/1/1/1/1/1/
*P74459A0136*
, Answer ALL questions.
Some questions must be answered with a cross in a box . If you change your mind about an
DO NOT WRITE IN THIS AREA DO NOT WRITE IN THIS AREA DO NOT WRITE IN THIS AREA
answer, put a line through the box and then mark your new answer with a cross .
1 Active transport is one type of cell transport mechanism.
(a) What happens during active transport?
(1)
A ATP is hydrolysed releasing energy
B ATP is hydrolysed using energy
C ATP is phosphorylated releasing energy
D ATP is phosphorylated using energy
(b) Which substance is transported against a concentration gradient by active
transport?
(1)
A hydrophilic molecule
B large hydrophobic molecule
C small hydrophobic molecule
D water
2
*P74459A0236*
, (c) The graph shows the change in concentration of molecule A and molecule B, as
they are transported into the cytoplasm of a cell.
DO NOT WRITE IN THIS AREA DO NOT WRITE IN THIS AREA DO NOT WRITE IN THIS AREA
molecule A
Concentration of molecules
in the cytoplasm molecule B
0 10 20 30
Time / mins
Explain the differences in the changes in concentration of molecule A and
molecule B between 0 and 10 minutes and between 20 and 30 minutes.
(2)
. . . . . . . . . . . .. .. .. .. .. .. .. .. .. .. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . ............................................................................................................................................ ................ . . . . . . . . . . . . . . . . . . . .
. . . . . . . . . . . .. .. .. .. .. .. .. .. .. .. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . ............................................................................................................................................ ................ . . . . . . . . . . . . . . . . . . . .
. . . . . . . . . . . .. .. .. .. .. .. .. .. .. .. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . ............................................................................................................................................ ................ . . . . . . . . . . . . . . . . . . . .
. . . . . . . . . . . .. .. .. .. .. .. .. .. .. .. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . ............................................................................................................................................ ................ . . . . . . . . . . . . . . . . . . . .
. . . . . . . . . . . .. .. .. .. .. .. .. .. .. .. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . ............................................................................................................................................ ................ . . . . . . . . . . . . . . . . . . . .
. . . . . . . . . . . .. .. .. .. .. .. .. .. .. .. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . ............................................................................................................................................ ................ . . . . . . . . . . . . . . . . . . . .
(Total for Question 1 = 4 marks)
3
*P74459A0336* Turn over
, 2 The diagram shows part of cellular respiration.
glucose
DO NOT WRITE IN THIS AREA DO NOT WRITE IN THIS AREA DO NOT WRITE IN THIS AREA
glycolysis
molecule X
link reaction
and
Krebs cycle
carbon dioxide
(a) Which is molecule X?
(1)
A glycerate 3-phosphate
B glycogen
C lactate
D pyruvate
(b) Which row of the table shows the locations of glycolysis, the link reaction and the
Krebs cycle in eukaryotic cells?
(1)
Glycolysis Link reaction Krebs cycle
A cytoplasm cytoplasm cytoplasm
B cytoplasm mitochondria mitochondria
C mitochondria cytoplasm cytoplasm
D mitochondria mitochondria mitochondria
4
*P74459A0436*
