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Lehninger Principles of Biochemistry – Test Bank (7th Edition, Nelson) – Chapters 1–28 – Complete Exam & Practice Question Collection

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This document contains a comprehensive test bank for Lehninger Principles of Biochemistry, 7th Edition, covering chapters 1 through 28. It includes hundreds of multiple-choice questions, short-answer items, and detailed conceptual prompts spanning cellular structure, biomolecules, metabolism, thermodynamics, genetics, evolution, and biochemical techniques. The answer keys for all question sets are included, making this file suitable for exam preparation and self-assessment.

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Institution
Principles of Biochemistry
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Uploaded on
November 14, 2025
Number of pages
631
Written in
2025/2026
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Exam (elaborations)
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Test Bank: Lehninger Principles of Biochemistry, 7th
Edition, David L. Nelson Chapters 1-28.

1. In a bacterial cell, the DNA is in the:
A) cell envelope.
B) cell membrane.
C) nucleoid.
D) nucleus.
E) ribosomes.


2. A major change occurring in the evolution of eukaryotes from prokaryotes was the
development of:
A) DNA.
a
B) photosynthetic capability.
i
C) plasma membranes.
D) ribosomes.
E) the nucleus.
t h
A) cell membrane.
b u
3. In eukaryotes, the nucleus is enclosed by a double membrane called the:

B) nuclear envelope.
C) nucleolus.
D) nucleoplasm. m
E) nucleosome.


4. The dimensions of living cells are limited, on the lower end by the minimum number of
biomolecules necessary for function, and on the upper end by the rate of diffusion of
solutes such as oxygen. Except for highly elongated cells, they usually have lengths and
diameters in the range of:
A) 0.1 m to 10 m.
B) 0.3 m to 30 m.
C) 0.3 m to 100 m.
D) 1 m to 100 m.
E) 1 m to 300 m.




Page 1

,5. Which group of single-celled microorganisms has many members found growing in
extreme environments?
A) bacteria
B) archaea
C) eukaryotes
D) heterotrophs
E) None of the answers is correct.


6. The bacterium E. coli requires simple organic molecules for growth and energy—it is
therefore a:
A) chemoautotroph.
B) chemoheterotroph.
C) lithotroph.
a
D) photoautotroph.
E) photoheterotroph.
i h
7. Which is a list of organelles?
A) mitochondria, chromatin, endoplasmic reticulum
t
B) peroxisomes, lysosomes, plasma membrane
u
C) proteasomes, peroxisomes, lysosomes
D) mitochondria, endoplasmic reticulum, peroxisomes
E) All of the answers are correct.
b
A) amino acid < protein < mitochondrion < ribosome
B) amino acid < protein < ribosome < mitochondrion
C) amino acid < ribosome < protein < mitochondrion
m
8. Which list has the cellular components arranged in order of INCREASING size?



D) protein < amino acid < mitochondrion < ribosome
E) protein < ribosome < mitochondrion < amino acid


9. The three-dimensional structure of macromolecules is formed and maintained primarily
through noncovalent interactions. Which one of the following is NOT considered a
noncovalent interaction?
A) carbon-carbon bonds
B) hydrogen bonds
C) hydrophobic interactions
D) ionic interactions
E) van der Waals interactions




Page 2

,10. Which element is NOT among the four most abundant in living organisms?
A) carbon
B) hydrogen
C) nitrogen
D) oxygen
E) phosphorus


11. The four covalent bonds in methane (CH4) are arranged around carbon to give which
geometry?
A) linear
B) tetrahedral
C) trigonal bipyramidal
D) trigonal planar
a
E) trigonal pyramidal
i
12. What functional groups are present on this molecule?
t h
b u
A) ether and aldehyde
B) hydroxyl and aldehyde
C) hydroxyl and carboxylic acid m
D) hydroxyl and ester
E) hydroxyl and ketone


13. The macromolecules that serve in the storage and transmission of genetic information
are:
A) carbohydrates.
B) lipids.
C) membranes.
D) nucleic acids.
E) proteins.


14. Stereoisomers that are nonsuperimposable mirror images of each other are known as:
A) anomers.
B) cis-trans isomers.
C) diastereoisomers.
D) enantiomers.
E) geometric isomers.



Page 3

, 15. The catalog of all proteins functioning in a cell is the:
A) metabolome.
B) proteasome.
C) lysosome.
D) proteome.
E) genome.

Answer Key
1. B
i a
2. D h
3. D
4. C
5. E
6. A
t u
7. B
8. D
9. E
10.
11.
E
D
b
12.
13.
14.
15.
A
D
A
A
m
16. C
17. B
18. C
19. A
20. D
21. B
22. C
23. D




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