CSN BIOL 251 FINAL EXAM 2025 LATEST FALL
SEMESTER 100% ACCURATE COLLEGE OF
SOUTHERN NEVADA
The temperature at which the organism grows best over time is ___________________.
Optimum
The temperature, below which the organism cannot grow is called the __________________.
Minimum
The temperature, above which the organism cannot grow is called the _________________.
Maximum
The optimum temperature of growth may be observed in broth cultures as measures of
_____________________.
Turbidity
The turbidity is measured by ___________________.
absorbance
What tool is used to measure the turbidity?
spectrophotometer
The higher the absorbance of light, the higher the growth, i.e. they are
______________________.
,proportional
As cells grow, more light is absorbed, and the higher the absorbance reading, this is also
referred to as what?
Optical Density
Organisms that grow only below 20 degrees C are classified as _________________________.
Psychrophiles
Organisms that live in the cold but can also grow up to 35 degrees C and slightly higher, are
classified as _____________________.
Psychrotrophs
Microbes that are adapted to temperatures between 15 degrees C and 45 degrees C are
classified as ________________________.
Mesophiles
Organisms growing above 45 degrees C - 80 degrees C are classified as
_________________________.
thermophiles
Microbes growing above 80 degrees C up to 110 degrees C are ___________________
_____________________.
extream thermophiles
These bacteria generally are mesophiles that endure high temperatures for short periods of
time.
, thermoduric
What two bacterial cultures were used for the Growth Factors Temperature Lab?
E. coli and Bacillus stearothermophilus
What were the four temperatures the bacteria were kept at for the Growth Factors
Temperature lab?
4, 25, 36, 55
The objective of the Growth Factors Temperature Lab is to quantify the amount of turbidity and
therefore the amount of bacterial growth by obtaining an _______________________ value for
each nutrient broth.
absorbance
What is the wavelength used to measure the absorbance of the bacteria of the Growth Factors
Temperature Lab?
550 nm
The availability of water is called the _______________ ________________, and values vary
between 0 and 1.0.
water activity
____________________, the movement of water across a membrane, occurs as a result of the
different solute concentrations on either side of the membrane.
Osmosis
SEMESTER 100% ACCURATE COLLEGE OF
SOUTHERN NEVADA
The temperature at which the organism grows best over time is ___________________.
Optimum
The temperature, below which the organism cannot grow is called the __________________.
Minimum
The temperature, above which the organism cannot grow is called the _________________.
Maximum
The optimum temperature of growth may be observed in broth cultures as measures of
_____________________.
Turbidity
The turbidity is measured by ___________________.
absorbance
What tool is used to measure the turbidity?
spectrophotometer
The higher the absorbance of light, the higher the growth, i.e. they are
______________________.
,proportional
As cells grow, more light is absorbed, and the higher the absorbance reading, this is also
referred to as what?
Optical Density
Organisms that grow only below 20 degrees C are classified as _________________________.
Psychrophiles
Organisms that live in the cold but can also grow up to 35 degrees C and slightly higher, are
classified as _____________________.
Psychrotrophs
Microbes that are adapted to temperatures between 15 degrees C and 45 degrees C are
classified as ________________________.
Mesophiles
Organisms growing above 45 degrees C - 80 degrees C are classified as
_________________________.
thermophiles
Microbes growing above 80 degrees C up to 110 degrees C are ___________________
_____________________.
extream thermophiles
These bacteria generally are mesophiles that endure high temperatures for short periods of
time.
, thermoduric
What two bacterial cultures were used for the Growth Factors Temperature Lab?
E. coli and Bacillus stearothermophilus
What were the four temperatures the bacteria were kept at for the Growth Factors
Temperature lab?
4, 25, 36, 55
The objective of the Growth Factors Temperature Lab is to quantify the amount of turbidity and
therefore the amount of bacterial growth by obtaining an _______________________ value for
each nutrient broth.
absorbance
What is the wavelength used to measure the absorbance of the bacteria of the Growth Factors
Temperature Lab?
550 nm
The availability of water is called the _______________ ________________, and values vary
between 0 and 1.0.
water activity
____________________, the movement of water across a membrane, occurs as a result of the
different solute concentrations on either side of the membrane.
Osmosis
