GSCU BACHOON MICROBIOLOGY LAB
FINAL EXAM 2025/2026 WITH ACTUAL
CORRECT QUESTIONS AND VERIFIED
DETAILED ANSWERS |CURRENTLY TESTING
QUESTIONS AND SOLUTIONS|ALREADY
GRADED A+|NEWEST|BRAND NEW VERSION
!!|GUARANTEED PASS
What is the purpose of using immersion oil?
more light is directed through the objective lenses and the image is more clear
What component of stain gives bacteria their color?
a colored chromophore group
What advantage does knowing the morphology and arrangement of a culture provide?
it can be used to diagnose the infectious organism and successfully treat it
What is a differential stain?
a method to distinguish between different types of bacteria, often based on color differences
What is the most critical step in the Gram staining procedure?
declorization
What is the advantage to the bacteria to produce endospores?
it can go dormant and last millions of years
After performing the acid-fast stain, what is the color of the cells lacking mycolic acid?
red/pink
If you were asked to isolate a bacteria from a water sample, which isolation technique would
you use and why?
spread plate; it can isolate bacteria from solutions
Can you relate urease production to diaper rash in infants?
1|Page
,urea reacts with bacteria on skin and leads to an increase in ammonia. Ammonia changes pH
and makes the baby more prone to diaper rashes.
Through what 5 ways can an individual acquire normal flora?
1. birth
2.eating
3.drinking
4. breathing
5.touching everyday objects
What green product worked best?
Growth Works
What brand worked best among the first aid/antiseptics?
Band Aid
What disinfectant brand worked the best?
Spick & Span
Which antibiotic was the most effective against the bacteria you plated?
Ampicillin, Tetracycline
a metabolic product of one organism that, in very small amounts, is detrimental or inhibitory to
microorganisms
antibiotic
According to health care experts, infectious diseases caused by ______________ are
responsible for more deaths worldwide than any other single cause.
microbes
Adequate precautions are necessary to prevent unwanted _________________ from getting in
or on experimental tubes or plates.
contaminants
What is the first step of aseptic technique?
Flame the inoculating loop
What is the second step of aseptic technique?
2|Page
, mix up organisms in culture
What is the third step of aseptic technique?
flame the mouth of the tube
What is the fourth step of aseptic technique?
remove organisms from tube using the inoculating loop.
What is the fifth step of aseptic technique?
remove loop from culture and flame the mouth of the tube and close the tube
What is the 6th step of aseptic technique?
transfer the loopful of bacteria to a test tube or Petri plate
What is the seventh step of aseptic technique?
After transferring, flame the loop
What bacteria did we use in the Aseptic Technique lab (Exercise 2)?
E. coli
used in carrying the microscope; houses the light source
Base
provides illumination to specimen
light source
along with the base, used as a carrying handle for the microscope
arm
platform which the specimen rests on
stage
controls the distance between the specimen and the stage
course and fine adjustment knobs
is used with scan and low power objective lenses
coarse focus
is used with high dry and oil immersion objective lenses
3|Page
FINAL EXAM 2025/2026 WITH ACTUAL
CORRECT QUESTIONS AND VERIFIED
DETAILED ANSWERS |CURRENTLY TESTING
QUESTIONS AND SOLUTIONS|ALREADY
GRADED A+|NEWEST|BRAND NEW VERSION
!!|GUARANTEED PASS
What is the purpose of using immersion oil?
more light is directed through the objective lenses and the image is more clear
What component of stain gives bacteria their color?
a colored chromophore group
What advantage does knowing the morphology and arrangement of a culture provide?
it can be used to diagnose the infectious organism and successfully treat it
What is a differential stain?
a method to distinguish between different types of bacteria, often based on color differences
What is the most critical step in the Gram staining procedure?
declorization
What is the advantage to the bacteria to produce endospores?
it can go dormant and last millions of years
After performing the acid-fast stain, what is the color of the cells lacking mycolic acid?
red/pink
If you were asked to isolate a bacteria from a water sample, which isolation technique would
you use and why?
spread plate; it can isolate bacteria from solutions
Can you relate urease production to diaper rash in infants?
1|Page
,urea reacts with bacteria on skin and leads to an increase in ammonia. Ammonia changes pH
and makes the baby more prone to diaper rashes.
Through what 5 ways can an individual acquire normal flora?
1. birth
2.eating
3.drinking
4. breathing
5.touching everyday objects
What green product worked best?
Growth Works
What brand worked best among the first aid/antiseptics?
Band Aid
What disinfectant brand worked the best?
Spick & Span
Which antibiotic was the most effective against the bacteria you plated?
Ampicillin, Tetracycline
a metabolic product of one organism that, in very small amounts, is detrimental or inhibitory to
microorganisms
antibiotic
According to health care experts, infectious diseases caused by ______________ are
responsible for more deaths worldwide than any other single cause.
microbes
Adequate precautions are necessary to prevent unwanted _________________ from getting in
or on experimental tubes or plates.
contaminants
What is the first step of aseptic technique?
Flame the inoculating loop
What is the second step of aseptic technique?
2|Page
, mix up organisms in culture
What is the third step of aseptic technique?
flame the mouth of the tube
What is the fourth step of aseptic technique?
remove organisms from tube using the inoculating loop.
What is the fifth step of aseptic technique?
remove loop from culture and flame the mouth of the tube and close the tube
What is the 6th step of aseptic technique?
transfer the loopful of bacteria to a test tube or Petri plate
What is the seventh step of aseptic technique?
After transferring, flame the loop
What bacteria did we use in the Aseptic Technique lab (Exercise 2)?
E. coli
used in carrying the microscope; houses the light source
Base
provides illumination to specimen
light source
along with the base, used as a carrying handle for the microscope
arm
platform which the specimen rests on
stage
controls the distance between the specimen and the stage
course and fine adjustment knobs
is used with scan and low power objective lenses
coarse focus
is used with high dry and oil immersion objective lenses
3|Page
