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HESI A2 Biology – Advanced Exam Practice PDF (2025) advanced HESI A2 biology questions and answers.

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The HESI A2 Biology – Advanced Exam is a comprehensive study resource for prospective nursing students preparing for the HESI Admission Assessment Exam (A2). This PDF includes multiple-choice, practice questions, and detailed answers covering essential biology topics such as cell structure, genetics, physiology, ecology, evolution, and human anatomy. Ideal for exam preparation, self-study, and review, this resource helps learners reinforce biological concepts, improve problem-solving and critical thinking skills, and increase confidence for the HESI A2 Biology exam. Updated for 2025, it aligns with current HESI exam standards and nursing admission requirements.

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Uploaded on
October 22, 2025
Number of pages
21
Written in
2025/2026
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HESI A2 Biology / Advanced HESI A2 Biology Actual
Exam with Correct Answers/ Updated 2024-2025.



Hierarchic Organizational system for nomenclature - ANSWER - Kingdom

Phylum

Class

Order

Family

Genus

Species (Least)

"Keep Penguins Cool Or Find Good Shelter"



Scientific Method - ANSWER - Observation

Hypothesis

Experiment

Conclusion



Water - ANSWER - All life, and therefore biology , occurs in water.



Properties of Water - ANSWER - 2 Hydrogen bonds Covalently bonded to Oxygen.



Hydrogen Bonding between molecules.

- High specific heat

- Large bodies or water stabilize climates

- Strong cohesive and adhesive properties

- Water freezes forms a lattice crystal resulting in ice floating in water.



pg. 1

,- Polarity of water allows it to act as a universal solvent.

- Water can be used to dissolve different solvents.



Carbohydrates - ANSWER - Long chains, or polymers of sugar. Most importantly form the backbone of
DNA and RNA.



Saturated - ANSWER - Clogs arteries. NO DOUBLE BONDS in their hydrocarbon tail. SOLID @ room
temperature.



Unsaturated - ANSWER - Double bond in their hydrocarbon tail. LIQUID at room temperature.



Phospholipids - ANSWER - Phosphate group (polar) soluble in water. Hydrocarbon tail of fatty acids is
nonpolar and nonsoluble in water.

Ex. Cell Membrane



Steroids - ANSWER - Are lipids, which are precursors to hormones and drugs



Proteins - ANSWER - They are Polymers of 20 molecules called Amino Acids. Enzymes are a type of
protein, which catalyze different reactions or processes. LARGEST Biological Molecule



Cell - ANSWER - Fundamental unit of biology



Prokaryotic Cells - ANSWER - NO defined nucleus and NO membrane- bound organelles.



Eukaryotic Cells - ANSWER - Membrane-enclosed nucleus and series of membrane-bound organelles that
carry out the functions of the cell as directed by the genetic information contained in the nucleus.



Nucleus - ANSWER - Contains DNA called Chromosomes.



Ribosomes - ANSWER - Are organelles that read the RNA produced in the nucleus and TRANSLATE the
genetic instructions to produce PROTEINS.



pg. 2

, Bound ribosomes - ANSWER - Are found attached to the endoplasmic reticulum (ER)



Free ribosomes - ANSWER - Are found in the cytoplasm



Endoplasmic reticulum - ANSWER - Attached to the nucleur membrane and consists of two continuous
parts.



Rough ER - ANSWER - Covered in RIBOSOMES and is responsible for PROTEIN SYNTHESIS and membrane
production.



Smooth ER - ANSWER - NO ribosomes. It functions in the DETOXification and METABOLISM of multiple
molecules.



Golgi Apparatus - ANSWER - Packaging, processing and shipping. "UPS"



Lysosomes - ANSWER - Intracellular DIGESTION. hydrolyze proteins, fats, sugars, and nucleic acids.



Vacuoles - ANSWER - uptake food through the cell membrane by phagocytosis. Plant cells have a central
vacuole that functions as storage, waste, disposal, protection, and hydrolysis



Mitochondria - ANSWER - Found in Eukaryotic cells and are the site of CELLULAR RESPIRATION



Cellular Respiration Formula - ANSWER - C6H12O2 + 6O2 ------> 6CO2 + 6H20 + Energy (ATP)



Steps of Aerobic Cellular Respiration - ANSWER - Glycolysis, Kreb's Cycle, Electron Chain Transport



Steps of Anaerobic Cellular Respiration - ANSWER - Glycolysis and Fermentation



Asexual Reproduction (Mitosis) - ANSWER - Produces 2 daughter cells. "Everything stays the same"



pg. 3
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