ASCP CG Certification Practice
A technique for - ANS-Immobilizing the tissue within the subculture vessel is known as
"Starring-In." A unmarried dish made from amniotic fluid will become cloudy 24 hours after the
start of the lifestyle. Which cultures should be discarded? - ANS-The one and only cloudy dish
46,XY,der(nine)(t(nine;10)(q32;q12). ANS-Unbalanced is the term that satisfactory describes
this written karyotype. ("der" refers to "a spinoff chromosome," and "t" refers to translocation).
This individual is unbalanced because it simplest has one replica of chromosome 9q (from band
9q33 to 9qter). In an amniotic flask, 50% of the cells have 92,XXYY, whilst the ultimate 50%
have forty six,XY. Probably, this supposed: - ANS-Pseudomosaicism A malignancy's
cytogenetics can most effective be studied with a blood pattern if: - ANS-Dividing leukemic cells
are gift within the bloodstream. A pattern of bone marrow that became taken in transport
medium is now leaking into the plastic bag that was used to transport it. The technologist have
to do the following: - ANS-While carrying gloves, smooth the tube. A change within the
concentration of CO2 effects in a pH change of 1 unit within the tradition medium. - ANS-10-fold
ANS-r(five)(p15.1q35) is a chromosome abnormality in a Cri-du-chat affected person. (The tip
ends of 5p and 5q on this ring chromosome are missing. Cri-du-chat syndrome effects from the
deletion of 5p15. Serum analytes in a fetus at expanded threat for trisomy 18 consist of:
ANS-low AFP, low hCG, and low uE3 Alpha fetoprotein, or AFP Human chorionic gonadotropin,
or hCG uE3 stands for estriol unconjugated. A exceptional guarantee software for a laboratory
includes the following: - ANS-Proficiency checking out The following signal sample changed into
furnished with the aid of a MLL fish: 1F, 1R, 1G. What is that this cellular's ISCN? It is
ANS-nucish (MLLx2)(five). MLL in Sep. MLLx1)
Chromosomes are overspread in a brand new hypotonic answer. How can the overspreading
be prevented? - ANS-Shorten the drying length If a regulatory inspector requests a replica of a
lab patient's document for his inspection documents, the technician need to: ANS-Refrain from
supplying the inspector with a copy of the record. See the coverage on HIPAA compliance.
G-banding analysis shows an inversion of chrom 9 to a technician. What sort of banding might
aid this? ANS-C banding Staining chromosomes with a banding approach that famous AT-rich
areas on chromosomes is the aim of a generation professional. Which approach of staining
have to he rent? - ANS-QFQ What is the minimal band stage for scientific specimens, in
keeping with CAP rules? - ANS-400 For most constitutional specimens, the endorsed degree is
550, however the lowest appropriate stage is four hundred. See law CYG.42200 of the CAP. A
cyto director believes that chrom 10 has a terminal del following G-banded evaluation.
ANS-FISH: Which banding technique might be maximum helpful in determining whether or not a
deletion has taken area? ANS: The condenser diaphragm need to be closed down by 1/4 to at
least one/3 after acting koehler illumination to make certain ultimate contrast among the light
and dark bands of the chroms. The chromosomes seem to have vague bands with little contrast
after staining. ANS-Increasing trypsin publicity (chroms were muddy and darkish) is the answer
to the difficulty. If all the cultures in a CO2 incubator failed, what aspects ought to be monitored
to determine the hassle's supply? - ANS-Temperature, CO2 content, and Level of humidity The
horizontal laminar waft hood possesses all of the following traits: - ANS-Protects the lifestyle
from contamination no longer appropriate to be used with transformed cell lines. Stymies the air
, that enters the hood. When getting ready the exceptional photograph, all the following
chromosomes have darkish bands on their chromosome regions: ANS-Chromosome 11p 12q
chromosome 17q chromosome Type M0 of AML is ANS-AML with little differentiation (no
chromosome change is associated). AML of the M1 kind—ANS-AML without a differentiation
and no related chromosomal modifications ANS-AML with differentiation within the M2 type of
AML t(8;21)(q22;q22)
Acute Promyelocytic Leukemia (APL) of the M3 sort of AML t(15;17)(q22;q11-12)
Acute Myelomonocytic Leukemia (AMML) of the M4 kind (ANS) inv(sixteen)(p13q22)
Type M5 Acute Monocytic Leukemia (ANS) t(nine;eleven)(p22;q23)
extra 11q23 reorganizations Type M6 of acute myeloid leukemia (AML) +8 at del(5q), del(7q).
The acute megakaryocytic leukemia (ANS) type of AML M7 (no chrom abn connections) After
the G and C bands, the prognosis for Case 777 isn't clear. The director thinks that the chrom
marker in question is attached to satellites. Which banding or stain need to be used to affirm
that the satellites in query are authentic? ANS-NOR banding After a chief repair, gadget should
be recalibrated according with CLIA 88. ANS: To make sure that it is nonetheless operational.
According to CLIA 1988 (Clinical Laboratory Improvement Amendments), equipment need to be
calibrated each six months (ANS) (Standard 493.1217). The ANS proteolytic enzymes
collagenase, pronase, and trypsin smash down tissues into unmarried-cell suspensions. After
an abnormality become detected by means of ultrasound at 12 weeks of pregnancy, cyto
studies were ordered on a prenatal affected person. Two of 15 colonies from awesome number
one cultures discovered an peculiar cellular line of forty seven,XX,+21. As that is a authentic
mosaic case, the subsequent step could be to: ANS-Report the end result and provide the
subsequent suggestions: continuing the amniocentesis, repeating a comprehensive ultrasound
or solicit a blood sample from the percutaneous umbilical wire (PUBS). A affected person with a
questionable leukemia analysis has asked cytogenetic studies. This affected person has had
chemotherapy and a blood transfusion, in addition to circulating myeloblasts and a low white cell
count number. ANS-PHA-inspired blood subculture is the least informative method. The
digicam's capacity to capture: - ANS-brightness is determined by using dynamic variety. What
kind of light supply can reason fluorescence? ANS-Mercury vapor lamps Each new batch of
hypotonic answer have to be evaluated by way of: - ANS-comparing the new lot to plenty this is
already in use for a success harvest. I usually use: ANS-An obj lens with a NA of 1.3 to at least
one.Four for chromosome imaging because I want the very best resolution viable. A analysis of:
- ANS-DiGenorge syndrome; - microdeletion of 22q11, additionally called velocardiofacial
syndrome; is indicated via excessive-decision banding. "velo" is a gentle color, "cardio" refers to
start defects inside the heart, "facial" refers to extraordinary bizarre facial functions like a short
philtrum (an indent inside the upper lip), a tubular nose, extensive-set eyes, and many others.
ANS: You can regulate the brightness of a microscope with the aid of including filters or
converting the regulating transformer. How many bands on chromosome 10 fall inside the
350-400 band stage variety? How many bands fall within the 425- to 475-band level range on
chromosome 10? - ANS-15 to 21 How many bands fall in the 500-550 band degree variety on
chromosome 10? - ANS-22 to 28 An 8-bit grayscale photo has how many brightness levels? -
ANS-256 ANS: Both corrosive and caustic chemical compounds ought to be saved in a
cupboard that is not flammable. When ought to an in-situ amniotic fluid culture be harvested? -
ANS-The number of colonies gift ANS-Consistent with trisomy 18 or a structural anomaly of
A technique for - ANS-Immobilizing the tissue within the subculture vessel is known as
"Starring-In." A unmarried dish made from amniotic fluid will become cloudy 24 hours after the
start of the lifestyle. Which cultures should be discarded? - ANS-The one and only cloudy dish
46,XY,der(nine)(t(nine;10)(q32;q12). ANS-Unbalanced is the term that satisfactory describes
this written karyotype. ("der" refers to "a spinoff chromosome," and "t" refers to translocation).
This individual is unbalanced because it simplest has one replica of chromosome 9q (from band
9q33 to 9qter). In an amniotic flask, 50% of the cells have 92,XXYY, whilst the ultimate 50%
have forty six,XY. Probably, this supposed: - ANS-Pseudomosaicism A malignancy's
cytogenetics can most effective be studied with a blood pattern if: - ANS-Dividing leukemic cells
are gift within the bloodstream. A pattern of bone marrow that became taken in transport
medium is now leaking into the plastic bag that was used to transport it. The technologist have
to do the following: - ANS-While carrying gloves, smooth the tube. A change within the
concentration of CO2 effects in a pH change of 1 unit within the tradition medium. - ANS-10-fold
ANS-r(five)(p15.1q35) is a chromosome abnormality in a Cri-du-chat affected person. (The tip
ends of 5p and 5q on this ring chromosome are missing. Cri-du-chat syndrome effects from the
deletion of 5p15. Serum analytes in a fetus at expanded threat for trisomy 18 consist of:
ANS-low AFP, low hCG, and low uE3 Alpha fetoprotein, or AFP Human chorionic gonadotropin,
or hCG uE3 stands for estriol unconjugated. A exceptional guarantee software for a laboratory
includes the following: - ANS-Proficiency checking out The following signal sample changed into
furnished with the aid of a MLL fish: 1F, 1R, 1G. What is that this cellular's ISCN? It is
ANS-nucish (MLLx2)(five). MLL in Sep. MLLx1)
Chromosomes are overspread in a brand new hypotonic answer. How can the overspreading
be prevented? - ANS-Shorten the drying length If a regulatory inspector requests a replica of a
lab patient's document for his inspection documents, the technician need to: ANS-Refrain from
supplying the inspector with a copy of the record. See the coverage on HIPAA compliance.
G-banding analysis shows an inversion of chrom 9 to a technician. What sort of banding might
aid this? ANS-C banding Staining chromosomes with a banding approach that famous AT-rich
areas on chromosomes is the aim of a generation professional. Which approach of staining
have to he rent? - ANS-QFQ What is the minimal band stage for scientific specimens, in
keeping with CAP rules? - ANS-400 For most constitutional specimens, the endorsed degree is
550, however the lowest appropriate stage is four hundred. See law CYG.42200 of the CAP. A
cyto director believes that chrom 10 has a terminal del following G-banded evaluation.
ANS-FISH: Which banding technique might be maximum helpful in determining whether or not a
deletion has taken area? ANS: The condenser diaphragm need to be closed down by 1/4 to at
least one/3 after acting koehler illumination to make certain ultimate contrast among the light
and dark bands of the chroms. The chromosomes seem to have vague bands with little contrast
after staining. ANS-Increasing trypsin publicity (chroms were muddy and darkish) is the answer
to the difficulty. If all the cultures in a CO2 incubator failed, what aspects ought to be monitored
to determine the hassle's supply? - ANS-Temperature, CO2 content, and Level of humidity The
horizontal laminar waft hood possesses all of the following traits: - ANS-Protects the lifestyle
from contamination no longer appropriate to be used with transformed cell lines. Stymies the air
, that enters the hood. When getting ready the exceptional photograph, all the following
chromosomes have darkish bands on their chromosome regions: ANS-Chromosome 11p 12q
chromosome 17q chromosome Type M0 of AML is ANS-AML with little differentiation (no
chromosome change is associated). AML of the M1 kind—ANS-AML without a differentiation
and no related chromosomal modifications ANS-AML with differentiation within the M2 type of
AML t(8;21)(q22;q22)
Acute Promyelocytic Leukemia (APL) of the M3 sort of AML t(15;17)(q22;q11-12)
Acute Myelomonocytic Leukemia (AMML) of the M4 kind (ANS) inv(sixteen)(p13q22)
Type M5 Acute Monocytic Leukemia (ANS) t(nine;eleven)(p22;q23)
extra 11q23 reorganizations Type M6 of acute myeloid leukemia (AML) +8 at del(5q), del(7q).
The acute megakaryocytic leukemia (ANS) type of AML M7 (no chrom abn connections) After
the G and C bands, the prognosis for Case 777 isn't clear. The director thinks that the chrom
marker in question is attached to satellites. Which banding or stain need to be used to affirm
that the satellites in query are authentic? ANS-NOR banding After a chief repair, gadget should
be recalibrated according with CLIA 88. ANS: To make sure that it is nonetheless operational.
According to CLIA 1988 (Clinical Laboratory Improvement Amendments), equipment need to be
calibrated each six months (ANS) (Standard 493.1217). The ANS proteolytic enzymes
collagenase, pronase, and trypsin smash down tissues into unmarried-cell suspensions. After
an abnormality become detected by means of ultrasound at 12 weeks of pregnancy, cyto
studies were ordered on a prenatal affected person. Two of 15 colonies from awesome number
one cultures discovered an peculiar cellular line of forty seven,XX,+21. As that is a authentic
mosaic case, the subsequent step could be to: ANS-Report the end result and provide the
subsequent suggestions: continuing the amniocentesis, repeating a comprehensive ultrasound
or solicit a blood sample from the percutaneous umbilical wire (PUBS). A affected person with a
questionable leukemia analysis has asked cytogenetic studies. This affected person has had
chemotherapy and a blood transfusion, in addition to circulating myeloblasts and a low white cell
count number. ANS-PHA-inspired blood subculture is the least informative method. The
digicam's capacity to capture: - ANS-brightness is determined by using dynamic variety. What
kind of light supply can reason fluorescence? ANS-Mercury vapor lamps Each new batch of
hypotonic answer have to be evaluated by way of: - ANS-comparing the new lot to plenty this is
already in use for a success harvest. I usually use: ANS-An obj lens with a NA of 1.3 to at least
one.Four for chromosome imaging because I want the very best resolution viable. A analysis of:
- ANS-DiGenorge syndrome; - microdeletion of 22q11, additionally called velocardiofacial
syndrome; is indicated via excessive-decision banding. "velo" is a gentle color, "cardio" refers to
start defects inside the heart, "facial" refers to extraordinary bizarre facial functions like a short
philtrum (an indent inside the upper lip), a tubular nose, extensive-set eyes, and many others.
ANS: You can regulate the brightness of a microscope with the aid of including filters or
converting the regulating transformer. How many bands on chromosome 10 fall inside the
350-400 band stage variety? How many bands fall within the 425- to 475-band level range on
chromosome 10? - ANS-15 to 21 How many bands fall in the 500-550 band degree variety on
chromosome 10? - ANS-22 to 28 An 8-bit grayscale photo has how many brightness levels? -
ANS-256 ANS: Both corrosive and caustic chemical compounds ought to be saved in a
cupboard that is not flammable. When ought to an in-situ amniotic fluid culture be harvested? -
ANS-The number of colonies gift ANS-Consistent with trisomy 18 or a structural anomaly of
