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CBSPD Final Exam – Latest 2025 Real Exam with 100+ Verified Questions and Correct Answers (Graded A, Brand New Version)

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This document provides the newest 2025 CBSPD (Certification Board for Sterile Processing and Distribution) Final Exam, featuring over 100 real exam questions with verified correct answers. The content covers all key domains of sterile processing, including decontamination, disinfection, sterilization methods, instrumentation, storage and distribution, infection prevention, and regulatory standards. Each question is paired with the correct answer to support accurate learning and exam readiness. Graded A and aligned with the latest exam version, this resource is a reliable tool for preparing with confidence for CBSPD certification.

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October 4, 2025
Number of pages
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Written in
2025/2026
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CBSPD Final Exam – Latest 2025 Real
Exam with 100+ Verified Questions
and Correct Answers (Graded A,
Brand New Version)

Cleaning, Decontamination, and Disinfection (Questions 1-25)

1. Question: What is the first step in manual cleaning of soiled instruments? Answer: Rinse
with lukewarm water to remove gross soil. Rationale: Prevents drying of bioburden;
AAMI ST79 (2017) recommends immediate rinse at point-of-use to avoid biofilm
formation (CBSPD 2025 Cleaning Module).



2. Question: Enzymatic detergents are used because they? Answer: Break down proteins,
starches, and fats. Rationale: Proteases for blood, amylases for starches; neutral pH
avoids corrosion (IAHCSMM CST manual, Ch. 4; 2025 update on eco-friendly
enzymes).



3. Question: What is the recommended water temperature for manual cleaning? Answer:
90-110°F (32-43°C). Rationale: Hotter water coagulates proteins; AAMI TIR34
guidelines for effective soil removal without damage.



4. Question: Ultrasonic cleaners should run for how long per load? Answer: 5-10 minutes.
Rationale: Allows cavitation to dislodge debris; over 10 min risks pitting (CBSPD
Ultrasonic Standards, 2025).



5. Question: High-level disinfection is required for? Answer: Semi-critical items like
endoscopes. Rationale: Kills all microbes except high numbers of spores (Spalding
classification; CDC HLD guidelines).

, 6. Question: What is the contact time for glutaraldehyde in HLD? Answer: 20 minutes at
20-25°C. Rationale: EPA label; temperature affects efficacy (AAMI ST91, flexible
endoscopes).



7. Question: PPE during decontamination includes? (Select all) Answer: Gloves, gown,
face shield, shoe covers. Rationale: OSHA BBP standard 1910.1030; protects against
splashes (2025 CBSPD PPE update).



8. Question: What causes biofilm in washers? Answer: Inadequate rinsing and drying.
Rationale: Mineral deposits harbor bacteria; weekly acid flush recommended (AAMI
ST79).



9. Question: Manual brushing should use? Answer: Nylon bristles and neutral detergent.
Rationale: Avoids scratches; soft metal tools for lumens (CBSPD Decon 2025).



10. Question: Pre-cleaning at point-of-use prevents? Answer: Soil drying and transport
contamination. Rationale: Within 30 min; reduces bioburden by 90% (AORN
Perioperative Standards).



11. Question: What is the purpose of a lumen brush? Answer: Remove debris from
channels. Rationale: Single-use preferred; size matches ID (AAMI ST91).



12. Question: Flash sterilization is for? Answer: Emergency unwrapped items only.
Rationale: Not routine; 3-min cycle at 132°C (AAMI ST79; 2025 caution on overuse).



13. Question: Disinfectant efficacy testing? Answer: Weekly ATP bioluminescence.
Rationale: Measures organic residue; <100 RLU clean (CBSPD QA).



14. Question: What is neutral pH? Answer: 7. Rationale: Safe for instruments; alkaline >8
corrodes (IAHCSMM manual).

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