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Biology 1001 Test 2 – 100+ Questions on DNA Replication, Transcription, Translation & Gene Expression – LSU 2025

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This document contains over 100 fully answered and explained questions from Biology 1001 Test 2 for the 2025/2026 academic year at Louisiana State University (LSU). The content provides a comprehensive review of nucleic acid structure, DNA replication, transcription, translation, and gene regulation in both prokaryotic and eukaryotic organisms. It's ideal for pre-med, pre-nursing, life sciences, molecular biology, and general biology students seeking mastery of molecular genetics. The guide begins with foundational concepts in DNA and RNA structure, including base pairing rules, antiparallel strand orientation, purines vs. pyrimidines, and sugar-phosphate backbones. It then explores DNA replication mechanisms, highlighting enzymes like DNA polymerase, helicase, primase, ligase, and topoisomerase, and the semi-conservative model of replication demonstrated by Meselson and Stahl. Transcription and RNA processing are covered in depth: RNA polymerase activity, promoter regions (including the TATA box), transcription factors, mRNA splicing, 5' caps, and poly-A tails. The translation process is explored with precise attention to codons, tRNA, rRNA, ribosomal subunits, initiation, elongation, termination, and post-translational modifications. Gene expression regulation is explained through operon models (lac and trp), chromatin remodeling, histone acetylation, enhancers, repressors, and epigenetic mechanisms. Advanced topics include telomere function and aging, mutations (missense, nonsense, frameshift), RNA types, and the universality of the genetic code. This test prep material is highly valuable for students preparing for unit tests, midterms, or final exams, and those pursuing genetics, cell biology, or biomedical programs. Keywords: DNA replication, RNA polymerase, gene expression, transcription, translation, codons, tRNA, ribosome, lac operon, trp operon, histone acetylation, telomeres, Okazaki fragments, helicase, ligase, nucleotides, exon, intron, mRNA, LSU

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Uploaded on
September 26, 2025
Number of pages
32
Written in
2025/2026
Type
Exam (elaborations)
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Questions & answers

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BIOLOGY 1001 TEST 2 2025/2026 EXAM
QUESTIONS AND ANSWERS | 100%
PASS



what is the primary role of DNA in living organisms? - 🧠ANSWER ✔✔to

store and transmit hereditary information

what type of reaction links nucleotides into a polynucleotide - 🧠ANSWER

✔✔dehydration reaction


what is the role of tRNA during protein synthesis - 🧠ANSWER ✔✔carry

amino acids to the ribosome

according to base pairing rules, which base pairs with guanine in DNA -

🧠ANSWER ✔✔cytosine

,what are the three components of a nucleotide - 🧠ANSWER

✔✔nitrogenous base, 5 carbon sugar, phosphate group


what direction are new polynucleotide chains synthesized - 🧠ANSWER

✔✔5' to 3'


in the RNA sequence 5'-AUGCUAACG-3' which of the bases are classified

as pyrimidines - 🧠ANSWER ✔✔Uracil and Cytosine


what type of bond links the sugars of two nucleotides in a polynucleotide -

🧠ANSWER ✔✔phosphodiester bond


what is true about tRNA - 🧠ANSWER ✔✔has a precise 3D shape due to

intrastrand base pairing

what feature of dna allows the generation of two identical copies during cell

division - 🧠ANSWER ✔✔double helix structure where one strand contains

the info to make the complementary strand

what's the term for the arrangement of the two sugar-phosphate backbones

in a DNA double helix - 🧠ANSWER ✔✔antiparallel


where does mRNA synthesis occur in a eukaryotic cell - 🧠ANSWER ✔✔in

the nucleus

,which nitrogenous base is found only in RNA and not in DNA - 🧠ANSWER

✔✔uracil


what is the repeating pattern of molecules in the backbone of a

polynucleotide - 🧠ANSWER ✔✔sugar-phosphate


during gene expression, what is the role of mRNA - 🧠ANSWER ✔✔to

convey genetic instructions from DNA to the ribosome


what is usually the final product of gene expression - 🧠ANSWER ✔✔protein


in the RNA sequence 5'-AUGCUAACG-3', which of the bases are classified

as purines? - 🧠ANSWER ✔✔adenine and guanine


in RNA, which base pairs with adenine - 🧠ANSWER ✔✔uracil


what is the structural difference between DNA and RNA - 🧠ANSWER

✔✔DNA is usually double stranded, RNA is usually single stranded


which process is directly controlled by DNA through an RNA intermediate -

🧠ANSWER ✔✔protein synthesis


what is the term for the entire process by which DNA directs the synthesis

of proteins - 🧠ANSWER ✔✔gene expression




COPYRIGHT©JOSHCLAY 2025/2026. YEAR PUBLISHED 2025. COMPANY REGISTRATION NUMBER: 619652435. TERMS OF USE. PRIVACY
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, in a DNA double helix, which base pairs with adenine - 🧠ANSWER

✔✔thymine


in RNA, which base pairs with guanine - 🧠ANSWER ✔✔cytosine


in the famous Hershy and Chase blender experiment, radioactive sulphur

was used to label ______ and radioactive phosphorus was used to level

_____. the experiment results demonstrated that the genetic material of a

bacteriophage is _______. - 🧠ANSWER ✔✔protein, DNA, DNA


the two strands that form a DNA double helix are joined to each other

through ______ - 🧠ANSWER ✔✔hydrogen bonds between complementary

nucleotide bases

working with streptococcus bacteria, Griffith showed that dead pathogenic

bacteria cells could ______ living bacteria cells that were ______ into

______ - 🧠ANSWER ✔✔transform, nonpathogenic, pathogenic


what was the conclusion of Griffith's experiment regarding the nature of the

transforming substance - 🧠ANSWER ✔✔it was an unknown heritable

substance

why were proteins initially considered better candidates for genetic material

than DNA - 🧠ANSWER ✔✔proteins were easier to study in the lab

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