Salsipaludibacter albus gen. nov., sp. nov., a novel actinobacterial strain isolate
from a Portuguese solar saltern and proposal of Salsipaludibacteraceae fam. nov.
and Salsipaludibacterales ord. nov.
Eduarda Almeida1,2,*, Valentina Henriques1, Sandra Wiegand3,4‡, Luciana Albuquerque5,
Peter Schumann6, Timo Kohn3, Christian Jogler3,7‡, Milton Simões da Costa5,8, Alexandre
Lobo-da-Cunha2,9, Maria de Fátima Carvalho2,9, Olga Maria Lage1,2
1
Faculty of Sciences, University of Porto (FCUP), Rua do Campo Alegre s/n, 4169-007
Porto, Portugal
2
Interdisciplinary Centre of Marine and Environmental Research of the University of
Porto (CIIMAR), Terminal de Cruzeiros do Porto de Leixões, Avenida General Norton de
Matos s/n, 4450-208 Matosinhos, Portugal
3
Department of Microbiology, Radboud University, Heyendaalseweg 135, Nijmegen, The
Netherlands
4
Institute for Biological Surfaces, Karlsruhe Institute of Technology, Hermann-von-
Helmholtz-Platz 1, 76344 Eggenstein-Leopoldshafen, Germany
CNC – Center for Neuroscience and Cell Biology, University of Coimbra, 3004-504
5
Coimbra, Portugal
Leibniz Institute DSMZ – German Collection of Microorganisms and Cell Cultures,
6
Braunschweig, Germany
7
Department of Microbial Interactions, Institute of Microbiology, Friedrich Schiller
University, Jena, Germany
8
Department of Life Sciences, University of Coimbra, Coimbra, Portugal.
9
Instituto de Ciências Biomédicas Abel Salazar, ICBAS, Universidade do Porto, Rua de
Jorge Viterbo Ferreira, 228, 4050-313 Porto, Portugal.
‡
Present address
*Corresponding author:
Keywords: new taxon, Salsipaludibacteraceae, Salsipaludibacterales, Salsipaludibacter
albus, Actinobacteria, saltern
Repositories: Sequences data were deposited at GenBank database under the
accession numbers MN031302 (16S rRNA gene) and JAHOPH000000000 (AS10T full
genome).
, Figure S1. AS10T colonies observed in M600 agar media.
Figure S2. AS10T growth M600 agar medium at different temperatures, ranging from 5 to 45 ºC with 5 ºC
increments and including also 37 and 55 ºC.
Figure S3. AS10T growth in M600 broth medium prepared with artificial seawater (ASW) without NaCl. Different
concentrations of NaCl, ranging from 0 to 15 % NaCl with 1 % NaCl increments, were tested. The horizontal line
represents the starting concentration of 0.5 McFarland. C+ stands for positive control, where AS10T was grown on
the isolation broth medium.
from a Portuguese solar saltern and proposal of Salsipaludibacteraceae fam. nov.
and Salsipaludibacterales ord. nov.
Eduarda Almeida1,2,*, Valentina Henriques1, Sandra Wiegand3,4‡, Luciana Albuquerque5,
Peter Schumann6, Timo Kohn3, Christian Jogler3,7‡, Milton Simões da Costa5,8, Alexandre
Lobo-da-Cunha2,9, Maria de Fátima Carvalho2,9, Olga Maria Lage1,2
1
Faculty of Sciences, University of Porto (FCUP), Rua do Campo Alegre s/n, 4169-007
Porto, Portugal
2
Interdisciplinary Centre of Marine and Environmental Research of the University of
Porto (CIIMAR), Terminal de Cruzeiros do Porto de Leixões, Avenida General Norton de
Matos s/n, 4450-208 Matosinhos, Portugal
3
Department of Microbiology, Radboud University, Heyendaalseweg 135, Nijmegen, The
Netherlands
4
Institute for Biological Surfaces, Karlsruhe Institute of Technology, Hermann-von-
Helmholtz-Platz 1, 76344 Eggenstein-Leopoldshafen, Germany
CNC – Center for Neuroscience and Cell Biology, University of Coimbra, 3004-504
5
Coimbra, Portugal
Leibniz Institute DSMZ – German Collection of Microorganisms and Cell Cultures,
6
Braunschweig, Germany
7
Department of Microbial Interactions, Institute of Microbiology, Friedrich Schiller
University, Jena, Germany
8
Department of Life Sciences, University of Coimbra, Coimbra, Portugal.
9
Instituto de Ciências Biomédicas Abel Salazar, ICBAS, Universidade do Porto, Rua de
Jorge Viterbo Ferreira, 228, 4050-313 Porto, Portugal.
‡
Present address
*Corresponding author:
Keywords: new taxon, Salsipaludibacteraceae, Salsipaludibacterales, Salsipaludibacter
albus, Actinobacteria, saltern
Repositories: Sequences data were deposited at GenBank database under the
accession numbers MN031302 (16S rRNA gene) and JAHOPH000000000 (AS10T full
genome).
, Figure S1. AS10T colonies observed in M600 agar media.
Figure S2. AS10T growth M600 agar medium at different temperatures, ranging from 5 to 45 ºC with 5 ºC
increments and including also 37 and 55 ºC.
Figure S3. AS10T growth in M600 broth medium prepared with artificial seawater (ASW) without NaCl. Different
concentrations of NaCl, ranging from 0 to 15 % NaCl with 1 % NaCl increments, were tested. The horizontal line
represents the starting concentration of 0.5 McFarland. C+ stands for positive control, where AS10T was grown on
the isolation broth medium.
