MICR290 Lab midterm
Blood agar plates also are used inside the microbiology labs as a diagnostic tool, which
organisms produce β hemolysis? Which organism produces α hemolysis? - ANS-Beta
hemolysis is full breakdown (flesh eating micro organism) - strep, pneumonia
Alpha is partial breakdown -
Gamma is not any breakage -
Compare and comparison Temperate Phages verses Virulent Phages? - ANS-Temperate
phages do not kill micro organism straight away after infection. Virulent phages smash bacterial
cells rapidly, following the lytic cycle. Temperate phages comply with both lytic and lysogenic
cycles
Describe aseptic approach and why is it crucial inside the scientific putting? Why is it important
within the microbiology lab? - ANS-Aseptic method is sterilization to limit contamination
Essential to hold unwanted microorganisms from contaminating cultures
Essential in lab to isolate natural tradition
Describe how Gel Electrophoresis is used to perceive micro organism. (Hint: how are the one of
a kind genes reduce or loaded and separated) - ANS-Separates molecules in DNA by means of
attracting negatively charged DNA closer to high-quality electrode, separates primarily based on
length, shape, acknowledged genes
Allows scientist to differentiate DNA of gene of hobby, ex: father of child, crime scene
Describe how Quadrant streak plating is performed to isolate person colonies of bacteria in the
lab. - ANS-Want to diagnose what patient has by using separating bacteria through diluting
organisms faraway from each other
Describe how Serial Dilution turned into used on this lab to rely the wide variety of plaques.
Compare and comparison Pour Plating with Serial Dilution. - ANS-The phages are grown in
liquid cultures or agar plates, lysis of the bacteria produces plaques which permits for estimation
of phage numbers by way of plaque assay
It is much like pour plating because they're each used to isolate bacterium, however serial
dilution may quantify the numbers whilst pour plating cannot.
Describe Pour Plating. Compare and contrast Pour Plating with Streak Plating. - ANS-Both used
to dilute/isolate
Pour plating: pour into take a look at tube, then pour into plate; can quantify from original
samples; can grow anaerobes
Streak plating: only use floor, can only see colonies, can not quantify; handiest aerobic
organisms
, Describe the Heat Fix Smear Technique. - ANS-Spread tradition in thin movie on the slide, air
dry, skip slide thru flame to fix it
How do Gram bad organisms look like with the Gram staining? What about the Gram effective?
Give an example of every micro organism. - ANS-Gram neg organisms have a red stain
(50-90% of cell wall is peptidoglycan). The gram pos organisms continue to be pink (10% of
cellular wall is peptidoglycan).
WBC presence indicates contamination
s. Aureus - gram pos
e. Coli - gram neg
How do you are making a smear from a Broth? How about from a plate? - ANS-From a broth,
droop contents from the tube, retrieve contents and vicinity onto slide
From a plate, placed water at the loop and scrape surface of agar to attain culture and region
onto slide
How does a dark subject microscope work? Which organisms are exceptional regarded with the
darkish subject microscope? - ANS-In darkish discipline microscopes, best mild rays are
scattered by means of specimen enter the goal lens so the specimen seems mild against a
darkish heritage—it will increase contrast and permits remark of greater info
Best for observing light objects
How does section evaluation microscope increase resolution? - ANS-Cause mild rays that
bypass thru a specimen to be out of segment with light rays that bypass thru the sector
producing comparison
How would S. Aureus look at the Manital Salt Agar Plates? - ANS-Would look yellow because of
the pH
If you are attempting to develop E. Coli (technology time every 20 minutes), how long would it
not take you to develop five,000 organisms? - ANS-4 hrs
(due to the fact 2>four>8>sixteen>32>sixty four>128>300>six hundred>1200>2400>5000
Count how many 20 minutes)
List 3 terrible stains. - ANS-Capsule staining, flagella staining, endospore staining
List the Biochemical Techniques? - ANS-PCR, DNA Microarrays, Gel Electrophoresis
List the traits of Algae. Compare & assessment the sexual duplicate in Algae and vegetation? -
ANS-Unicellular or multicellular
Photosynthetic
Simple reproductive structures
Categorized on pigmentation and composition of mobile wall
Blood agar plates also are used inside the microbiology labs as a diagnostic tool, which
organisms produce β hemolysis? Which organism produces α hemolysis? - ANS-Beta
hemolysis is full breakdown (flesh eating micro organism) - strep, pneumonia
Alpha is partial breakdown -
Gamma is not any breakage -
Compare and comparison Temperate Phages verses Virulent Phages? - ANS-Temperate
phages do not kill micro organism straight away after infection. Virulent phages smash bacterial
cells rapidly, following the lytic cycle. Temperate phages comply with both lytic and lysogenic
cycles
Describe aseptic approach and why is it crucial inside the scientific putting? Why is it important
within the microbiology lab? - ANS-Aseptic method is sterilization to limit contamination
Essential to hold unwanted microorganisms from contaminating cultures
Essential in lab to isolate natural tradition
Describe how Gel Electrophoresis is used to perceive micro organism. (Hint: how are the one of
a kind genes reduce or loaded and separated) - ANS-Separates molecules in DNA by means of
attracting negatively charged DNA closer to high-quality electrode, separates primarily based on
length, shape, acknowledged genes
Allows scientist to differentiate DNA of gene of hobby, ex: father of child, crime scene
Describe how Quadrant streak plating is performed to isolate person colonies of bacteria in the
lab. - ANS-Want to diagnose what patient has by using separating bacteria through diluting
organisms faraway from each other
Describe how Serial Dilution turned into used on this lab to rely the wide variety of plaques.
Compare and comparison Pour Plating with Serial Dilution. - ANS-The phages are grown in
liquid cultures or agar plates, lysis of the bacteria produces plaques which permits for estimation
of phage numbers by way of plaque assay
It is much like pour plating because they're each used to isolate bacterium, however serial
dilution may quantify the numbers whilst pour plating cannot.
Describe Pour Plating. Compare and contrast Pour Plating with Streak Plating. - ANS-Both used
to dilute/isolate
Pour plating: pour into take a look at tube, then pour into plate; can quantify from original
samples; can grow anaerobes
Streak plating: only use floor, can only see colonies, can not quantify; handiest aerobic
organisms
, Describe the Heat Fix Smear Technique. - ANS-Spread tradition in thin movie on the slide, air
dry, skip slide thru flame to fix it
How do Gram bad organisms look like with the Gram staining? What about the Gram effective?
Give an example of every micro organism. - ANS-Gram neg organisms have a red stain
(50-90% of cell wall is peptidoglycan). The gram pos organisms continue to be pink (10% of
cellular wall is peptidoglycan).
WBC presence indicates contamination
s. Aureus - gram pos
e. Coli - gram neg
How do you are making a smear from a Broth? How about from a plate? - ANS-From a broth,
droop contents from the tube, retrieve contents and vicinity onto slide
From a plate, placed water at the loop and scrape surface of agar to attain culture and region
onto slide
How does a dark subject microscope work? Which organisms are exceptional regarded with the
darkish subject microscope? - ANS-In darkish discipline microscopes, best mild rays are
scattered by means of specimen enter the goal lens so the specimen seems mild against a
darkish heritage—it will increase contrast and permits remark of greater info
Best for observing light objects
How does section evaluation microscope increase resolution? - ANS-Cause mild rays that
bypass thru a specimen to be out of segment with light rays that bypass thru the sector
producing comparison
How would S. Aureus look at the Manital Salt Agar Plates? - ANS-Would look yellow because of
the pH
If you are attempting to develop E. Coli (technology time every 20 minutes), how long would it
not take you to develop five,000 organisms? - ANS-4 hrs
(due to the fact 2>four>8>sixteen>32>sixty four>128>300>six hundred>1200>2400>5000
Count how many 20 minutes)
List 3 terrible stains. - ANS-Capsule staining, flagella staining, endospore staining
List the Biochemical Techniques? - ANS-PCR, DNA Microarrays, Gel Electrophoresis
List the traits of Algae. Compare & assessment the sexual duplicate in Algae and vegetation? -
ANS-Unicellular or multicellular
Photosynthetic
Simple reproductive structures
Categorized on pigmentation and composition of mobile wall
