1|Page
BCHM 4360 EXAM 2 NEWEST 2025/2026 ACTUAL EXAM WITH
COMPLETE QUESTIONS AND CORRECT DETAILED ANSWERS (100%
VERIFIED ANSWERS) |ALREADY GRADED A+| ||PROFESSOR
VERIFIED||
What are three other elements besides the TBP or TATA box that
are often present in eukaryotic transcription? - ANSWER-TFIIB
recognition element (BRE), initiator element (INR), and
downstream promoter element (DPE)
True or False: A TATA box is required for every single
transcription event. - ANSWER-False, although it is found about
95% of the time
What is the first step in assembling the transcription initiation
complex? - ANSWER-Binding of TFIID to the TATA box
How does TFIID bind to the TATA box? - ANSWER-Via TBP,
which binds to the minor groove of DNA, which induces strong
distortions in the DNA and causes local DNA unwinding
,2|Page
Some other components of TFIID, called TBP-_________ factors
(TAFs) mediate recognition of other promoter elements like ___
and ___. - ANSWER-associated, INR, DPE
What is the next step in transcription initiation after TFIID has
associated with DNA? - ANSWER-TFIIB is recruited. This
recognizes the BRE promoter element and binds asymmetrically,
helping to determine the transcription direction.
What is the next step in transcription initiation after TFIIB has
bound? - ANSWER-TFIIA binds and stabilizes the TBP-DNA
interactions.
What is the next step in transcription initiation after TFIIA binds? -
ANSWER-TFIIE and TFIIH bind, which catalyze ATP-powered
unwinding of DNA
What are the two main components of the pre-inititation complex?
- ANSWER-RNA Polymerase II and general transcription factors
,3|Page
True or False: The pre-initiation complex is good enough to
initiate transcription in vitro and in vivo. - ANSWER-False, in vivo
requires additional protein complexes
Another large complex, ________, is needed to activate many
Polymerase II transcribed genes. - ANSWER-mediator
What are the main similarities between Pol I, II, and III? -
ANSWER-Core subunits are similar, all three use TBP to initiate
transcription
What are the main differences between Pol I, II, and III? -
ANSWER-TBP is part of different complexes in each enzyme,
each use different proteins to initiate transcription, recognize
different promoters, and have different general transcription
factors
Once RNA Polymerase is in position, the complex of polymerase
and promoter is called a ______ complex. - ANSWER-closed
, 4|Page
Approximatel how many bp of duplex DNA (transcription bubble)
does RNA Polymerase open up? - ANSWER-14bp
True or False: ATP is required to open up the transcription bubble
in all three RNA Polymerases - ANSWER-False, only Pol II
requires ATP
The RNA Polymerase bound to an open region of DNA is called
the ____ complex. - ANSWER-open
RNA polymerase frequently fails to make a full length RNA on the
first attempt, which leads to the release of short RNAs of 2-9
nucleotides, and is called ________ initiation. - ANSWER-
abortive
______________ is the key to release the tether attached to RNA
Polymerase so that RNA Polymerase can get going like it wants
to. - ANSWER-phosphorylation
BCHM 4360 EXAM 2 NEWEST 2025/2026 ACTUAL EXAM WITH
COMPLETE QUESTIONS AND CORRECT DETAILED ANSWERS (100%
VERIFIED ANSWERS) |ALREADY GRADED A+| ||PROFESSOR
VERIFIED||
What are three other elements besides the TBP or TATA box that
are often present in eukaryotic transcription? - ANSWER-TFIIB
recognition element (BRE), initiator element (INR), and
downstream promoter element (DPE)
True or False: A TATA box is required for every single
transcription event. - ANSWER-False, although it is found about
95% of the time
What is the first step in assembling the transcription initiation
complex? - ANSWER-Binding of TFIID to the TATA box
How does TFIID bind to the TATA box? - ANSWER-Via TBP,
which binds to the minor groove of DNA, which induces strong
distortions in the DNA and causes local DNA unwinding
,2|Page
Some other components of TFIID, called TBP-_________ factors
(TAFs) mediate recognition of other promoter elements like ___
and ___. - ANSWER-associated, INR, DPE
What is the next step in transcription initiation after TFIID has
associated with DNA? - ANSWER-TFIIB is recruited. This
recognizes the BRE promoter element and binds asymmetrically,
helping to determine the transcription direction.
What is the next step in transcription initiation after TFIIB has
bound? - ANSWER-TFIIA binds and stabilizes the TBP-DNA
interactions.
What is the next step in transcription initiation after TFIIA binds? -
ANSWER-TFIIE and TFIIH bind, which catalyze ATP-powered
unwinding of DNA
What are the two main components of the pre-inititation complex?
- ANSWER-RNA Polymerase II and general transcription factors
,3|Page
True or False: The pre-initiation complex is good enough to
initiate transcription in vitro and in vivo. - ANSWER-False, in vivo
requires additional protein complexes
Another large complex, ________, is needed to activate many
Polymerase II transcribed genes. - ANSWER-mediator
What are the main similarities between Pol I, II, and III? -
ANSWER-Core subunits are similar, all three use TBP to initiate
transcription
What are the main differences between Pol I, II, and III? -
ANSWER-TBP is part of different complexes in each enzyme,
each use different proteins to initiate transcription, recognize
different promoters, and have different general transcription
factors
Once RNA Polymerase is in position, the complex of polymerase
and promoter is called a ______ complex. - ANSWER-closed
, 4|Page
Approximatel how many bp of duplex DNA (transcription bubble)
does RNA Polymerase open up? - ANSWER-14bp
True or False: ATP is required to open up the transcription bubble
in all three RNA Polymerases - ANSWER-False, only Pol II
requires ATP
The RNA Polymerase bound to an open region of DNA is called
the ____ complex. - ANSWER-open
RNA polymerase frequently fails to make a full length RNA on the
first attempt, which leads to the release of short RNAs of 2-9
nucleotides, and is called ________ initiation. - ANSWER-
abortive
______________ is the key to release the tether attached to RNA
Polymerase so that RNA Polymerase can get going like it wants
to. - ANSWER-phosphorylation
