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ASCP Clinical Chemistry Study Guide 2025/2026 – 300+ Key Concepts, Formulas & Practice Q&A | Electrolytes, Enzymes, Acid-Base, Toxicology, QA/QC & More

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This complete study guide is designed for candidates preparing for the ASCP Board of Certification (BOC) exams in 2025/2026, focusing exclusively on Clinical Chemistry. It contains 300+ essential points, including concise definitions, key lab values, diagnostic formulas, and exam-style practice questions with correct answers. Whether you're reviewing for the MLS, MLT, or ASCPi certification, this guide offers high-yield content organized by topic to boost exam readiness. Topics covered include: Electrolyte balance & calculations (anion gap, osmolality, sodium, potassium, chloride, bicarbonate) Carbohydrates, lipids, proteins & enzymes (glucose metabolism, cardiac markers, liver function tests) Acid-base disturbances (metabolic/respiratory acidosis & alkalosis) Toxicology & therapeutic drug monitoring (digoxin, lithium, acetaminophen, salicylates, drug interferences) Hormones & endocrine function (thyroid panel, adrenal tests, reproductive hormones) Quality assurance, quality control (QA/QC), method validation, reference ranges, and troubleshooting Each section is aligned with ASCP content outlines and clinical laboratory standards to ensure you’re studying what matters most. Tables, memory aids, and simplified concepts make it ideal for both structured review and last-minute revision. Relevant for: Medical Laboratory Scientist (MLS) students Medical Laboratory Technician (MLT) candidates Clinical Chemistry-focused Biomedical Science students ASCPi international exam-takers Healthcare professionals seeking certification or CE in clinical chemistry This guide is especially useful for those who need a solid grasp of analytical techniques, diagnostic interpretation, and clinical correlations in the chemistry section of the exam. Keywords: clinical chemistry, ASCP chemistry study guide, electrolytes, acid-base balance, enzymes, cardiac markers, liver function, lipids, endocrine testing, toxicology, therapeutic drugs, QA QC, reference ranges, analytes, ASCP MLT, ASCP MLS, ASCPi chemistry, lab values, BOC review

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ASCP Complete Chemistry Study Guide
2025/2026 Exam Questions and Answers
| 100% Pass



CARBOHYDRATES




1. Following overnight fasting, hypoglycemia in adults is defined as a

glucose of: - 🧠ANSWER ✔✔<45 mg/dL (<2.5 mmol/L)


2. The following results are from a 21 year-old patient with a back injury

who appears otherwise healthy:




Whole Blood Glucose: 77 mg/dL (4.2 mmol/L)

Serum Glucose: 88 mg/dL (4.8 mmol/L)

CSF Glucose: 56 mg/dL (3.1 mmol/L

,The best interpretation of these results is that: - 🧠ANSWER ✔✔All values

are consistent with a normal healthy individual

3. The preparation of a patient for standard glucose tolerance testing

should include: - 🧠ANSWER ✔✔A high carbohydrate diet for 3 days


4. If a fasting glucose was 90 mg/dL, which of the following 2-hour

postprandial glucose results would most closely represent normal glucose

metabolism? - 🧠ANSWER ✔✔100 mg/dL (5.5 mmol/L)


5. A healthy person with a blood glucose of 80 mg/dL (4.4 mmol/L) would

have a simultaneously determined cerebrospinal fluid glucose value of: -

🧠ANSWER ✔✔50 mg/dL (2.3 mmol/L)


6. A 25-year -old man became nauseated and vomited 90 minutes after

receiving a standard 75 g carbohydrate dose for an oral glucose tolerance

test. The best course of action is to: - 🧠ANSWER ✔✔Draw blood for

glucose and discontinue test

7. Cerebrospinal fluid for glucose assay should be: - 🧠ANSWER

✔✔Analyzed immediately

,8. Which of the following 2-hour postprandial glucose values demonstrates

unequivocal hyperglycemia diagnostic for diabetes mellitus? - 🧠ANSWER

✔✔200 mg/dL (11.0 mmol/L)


9. Serum levels that define hypoglycemia in pre-term or low birth weight

infants are: - 🧠ANSWER ✔✔Lower than adults


10. A 45-year-old woman has a fasting serum glucose concentration of 95

mg/dL (5.2 mmol/L) and a 2-hour postprandial glucose concentration of 105

mg/dL (5.8 mmol/L). The statement which best describes this patient's

fasting serum glucose concentration is: - 🧠ANSWER ✔✔Normal; reflecting

glycogen breakdown by the liver

11. Pregnant women with symptoms of thirst, frequent urination or

unexplained weight loss would have which of the following tests

performed? - 🧠ANSWER ✔✔Glucose tolerance test


12. In the fasting state, the arterial and capillary blood glucose

concentration varies from the venous glucose concentration by

approximately how many mg/dL (mmol/L)? - 🧠ANSWER ✔✔5 mg/dL (0 .27

mmol/L) higher




3
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, 13. The conversion of glucose or other hexoses into lactate or pyruvate is

called : - 🧠ANSWER ✔✔Glycolysis


14. Which one of the following values obtained during a glucose tolerance

test are diagnostic of diabetes mellitus? - 🧠ANSWER ✔✔Fasting plasma

glucose = 126 mg/dL (6.9 mmol/L)

15. The glycated hemoglobin value represents the integrated values of

glucose concentration during the preceding : - 🧠ANSWER ✔✔6-8 weeks


16. Monitoring long-term glucose control in patient s with adult onset

diabetes mellitus can best be accomplished by measuring: - 🧠ANSWER

✔✔Hgb A1C


17. A patient with Type I, insulin-dependent diabetes mellitus has the

following results:




Test Patient Referenced range

FBS: 150 mg/dL (8.3 mmol/dL) 70-110 mg/dL (mmol/dL)

Hgb A1C: 8.5% 4.0 % - 6.0%

Fructosamine: 2.5 mmol/dL 2.0 - 2.9 mmol/dL

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