ASCP CG Certification Practice
2025/2026 Exam Questions and Correct
Answers | New Update
What is the best agent to use against mycoplasma? - 🧠ANSWER ✔✔BM
cyclin - a combination of two antibiotics (tiamulin and minocycline) that
effecticely targets mycoplasma growth
For successful harvest, each new batch of hypotonic solution should be
evaluated by: - 🧠ANSWER ✔✔comparing the new lot to a lot already in use
Colagenase, pronase, and trypsin are: - 🧠ANSWER ✔✔Proteolytic
enzymes - break down tissues into single-cell suspensions
When harvesting peripheral blood cultures, asceptic technique no longer
matters following incubation with: - 🧠ANSWER ✔✔Colcemid - mitotic
spindle poison that halts cells at metaphase
,Samples that arrive at the cytogenetics lab should first be: - 🧠ANSWER
✔✔Logged in to the system
Phytohemagglutinin (PHA) stimulates which type of cells into division? -
🧠ANSWER ✔✔T-cell lymphocytes
To avoid culture failure, monitoring reagents is crucial. When logging a
culture failure, what is the MOST important to document? - 🧠ANSWER
✔✔Expiration dates of reagents used
Which type of microscope is used when cleaning a CVS (chorionic villus)
sample? - 🧠ANSWER ✔✔Dissection
Which cell type is most problematic if found in a CVS (chorionic villus)
sample? - 🧠ANSWER ✔✔Decidua (maternal tissue)
When looking at a slide under a phase contrast scope and a lot of
cytoplasm is still seen around the mets, what is the most likely cause? -
🧠ANSWER ✔✔innappropriate slide drying time
What will help prevent microbial contamination and cross contamination of
cultures? - 🧠ANSWER ✔✔Asceptic technique
,When there are not enough mets to complete the study of a patient/case,
what needs to be done next? - 🧠ANSWER ✔✔prepare additional slides
from any remaining sample
When looking at a dropped slide under a phase scope and mets are very
spread out and sparse, what could help fix the issue? - 🧠ANSWER
✔✔Increading the drying time by using warm slides
What is true of an inverted microscope? - 🧠ANSWER ✔✔the objectives are
beneath the stage
High-resolution banding is indicated for a possible diagnosis of: -
🧠ANSWER ✔✔DiGenorge syndrome -- microdeletion of 22q11, also called
velocardiofacial syndrome
"velo" - soft palette,
"cardio" - congenital heart defects,
"facial" - unique abnormal facial features, short philtrum (upper lip indent),
tubular nose, wide spread eyes, etc
3
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STATEMENT. ALL RIGHTS RESERVED
, When verifying if a specimen is appropriate for cytogenetic analysis, what
is the MOST important data to accompany the specimen? - 🧠ANSWER
✔✔Reason for referral
Which type of specimen should be requested for a cytogenetic analysis for
turner syndrome? - 🧠ANSWER ✔✔Heparinized peripheral blood stored at
room temperature
(NOT FROZEN)
A blood sample can be used to study the cytogenetics of a malignancy only
if: - 🧠ANSWER ✔✔Dividing leukemic cells are present in circulation
All of the cultures in a CO2 incubator failed, what aspects of the incubator
are important to monitor to assess the source of the problem? - 🧠ANSWER
✔✔Temperature,
CO2 level, and
Humidity level
2025/2026 Exam Questions and Correct
Answers | New Update
What is the best agent to use against mycoplasma? - 🧠ANSWER ✔✔BM
cyclin - a combination of two antibiotics (tiamulin and minocycline) that
effecticely targets mycoplasma growth
For successful harvest, each new batch of hypotonic solution should be
evaluated by: - 🧠ANSWER ✔✔comparing the new lot to a lot already in use
Colagenase, pronase, and trypsin are: - 🧠ANSWER ✔✔Proteolytic
enzymes - break down tissues into single-cell suspensions
When harvesting peripheral blood cultures, asceptic technique no longer
matters following incubation with: - 🧠ANSWER ✔✔Colcemid - mitotic
spindle poison that halts cells at metaphase
,Samples that arrive at the cytogenetics lab should first be: - 🧠ANSWER
✔✔Logged in to the system
Phytohemagglutinin (PHA) stimulates which type of cells into division? -
🧠ANSWER ✔✔T-cell lymphocytes
To avoid culture failure, monitoring reagents is crucial. When logging a
culture failure, what is the MOST important to document? - 🧠ANSWER
✔✔Expiration dates of reagents used
Which type of microscope is used when cleaning a CVS (chorionic villus)
sample? - 🧠ANSWER ✔✔Dissection
Which cell type is most problematic if found in a CVS (chorionic villus)
sample? - 🧠ANSWER ✔✔Decidua (maternal tissue)
When looking at a slide under a phase contrast scope and a lot of
cytoplasm is still seen around the mets, what is the most likely cause? -
🧠ANSWER ✔✔innappropriate slide drying time
What will help prevent microbial contamination and cross contamination of
cultures? - 🧠ANSWER ✔✔Asceptic technique
,When there are not enough mets to complete the study of a patient/case,
what needs to be done next? - 🧠ANSWER ✔✔prepare additional slides
from any remaining sample
When looking at a dropped slide under a phase scope and mets are very
spread out and sparse, what could help fix the issue? - 🧠ANSWER
✔✔Increading the drying time by using warm slides
What is true of an inverted microscope? - 🧠ANSWER ✔✔the objectives are
beneath the stage
High-resolution banding is indicated for a possible diagnosis of: -
🧠ANSWER ✔✔DiGenorge syndrome -- microdeletion of 22q11, also called
velocardiofacial syndrome
"velo" - soft palette,
"cardio" - congenital heart defects,
"facial" - unique abnormal facial features, short philtrum (upper lip indent),
tubular nose, wide spread eyes, etc
3
COPYRIGHT©JOSHCLAY 2025/2026. YEAR PUBLISHED 2025. COMPANY REGISTRATION NUMBER: 619652435. TERMS OF USE. PRIVACY
STATEMENT. ALL RIGHTS RESERVED
, When verifying if a specimen is appropriate for cytogenetic analysis, what
is the MOST important data to accompany the specimen? - 🧠ANSWER
✔✔Reason for referral
Which type of specimen should be requested for a cytogenetic analysis for
turner syndrome? - 🧠ANSWER ✔✔Heparinized peripheral blood stored at
room temperature
(NOT FROZEN)
A blood sample can be used to study the cytogenetics of a malignancy only
if: - 🧠ANSWER ✔✔Dividing leukemic cells are present in circulation
All of the cultures in a CO2 incubator failed, what aspects of the incubator
are important to monitor to assess the source of the problem? - 🧠ANSWER
✔✔Temperature,
CO2 level, and
Humidity level
