BIOCHEM C785 FINAL EXAM QUESTIONS AND CORRECT
ANSWERS, WITH COMPLETE SOLUTION.
Which mRNA sequence results in a polypeptide that contains three amino acids?
5' - CUU GAU ACU UAG - 3'
Which RNA sequence would end in the following polypeptide?
Phe-Thr-Val-Stop
5' - UUC ACA GUA UAA - 3'
What is the complementary sequence for the following DNA sequence?
5' - GCC ATC TCG AAT - 3'
5' - ATT CGA GAT GGC - 3'
Assuming 100% reaction efficiency, how many DNA copies are created after the
completion of four complete PCR cycles?
16
What is the function of DNA polymerase in the process of PCR?
It recognizes the primers and uses the available dNTPs to replicate the template DNA
sequence.
5' - [...] ATT CGC TAG GGC [...] - 3' − Normal DNA
5' - [...] ATT CGC GAG GGC [...] - 3' − Mutant DNA
Which type of mutation and outcome characterizes the Mutant DNA?
A missense mutation leading to a longer protein
In sickle-cell disease, the glutamic acid (Glu) at position number seven is mutated
to valine (Val).
What change in codons corresponds to this mutation?
GAA to GTA
What are possible blood types of the parents of a patient with blood type AB?
A and B
Which pair of chromosomes accurately depicts the genotype of a person with an
autosomal recessive mutant HBB allele and sickle cell anemia?
option 3- 2 mutant genes on chromosome 11
Which pair of chromosomes accurately depicts the genotype of a person with an
X-linked recessive mutant ALAS2 allele and sideroblastic anemia?
option 3, mutated allele
Studies have shown that increased expression of two genes called SIRT1 and
SIRT2 is associated with chronic exposure to cocaine and contributes to drug
addiction.
What difference in the regions of the SIRT1 and SIRT2 genes in people addicted
to cocaine increases their expression?
The nucleosomes become more widely spaced.
ANSWERS, WITH COMPLETE SOLUTION.
Which mRNA sequence results in a polypeptide that contains three amino acids?
5' - CUU GAU ACU UAG - 3'
Which RNA sequence would end in the following polypeptide?
Phe-Thr-Val-Stop
5' - UUC ACA GUA UAA - 3'
What is the complementary sequence for the following DNA sequence?
5' - GCC ATC TCG AAT - 3'
5' - ATT CGA GAT GGC - 3'
Assuming 100% reaction efficiency, how many DNA copies are created after the
completion of four complete PCR cycles?
16
What is the function of DNA polymerase in the process of PCR?
It recognizes the primers and uses the available dNTPs to replicate the template DNA
sequence.
5' - [...] ATT CGC TAG GGC [...] - 3' − Normal DNA
5' - [...] ATT CGC GAG GGC [...] - 3' − Mutant DNA
Which type of mutation and outcome characterizes the Mutant DNA?
A missense mutation leading to a longer protein
In sickle-cell disease, the glutamic acid (Glu) at position number seven is mutated
to valine (Val).
What change in codons corresponds to this mutation?
GAA to GTA
What are possible blood types of the parents of a patient with blood type AB?
A and B
Which pair of chromosomes accurately depicts the genotype of a person with an
autosomal recessive mutant HBB allele and sickle cell anemia?
option 3- 2 mutant genes on chromosome 11
Which pair of chromosomes accurately depicts the genotype of a person with an
X-linked recessive mutant ALAS2 allele and sideroblastic anemia?
option 3, mutated allele
Studies have shown that increased expression of two genes called SIRT1 and
SIRT2 is associated with chronic exposure to cocaine and contributes to drug
addiction.
What difference in the regions of the SIRT1 and SIRT2 genes in people addicted
to cocaine increases their expression?
The nucleosomes become more widely spaced.
