MB (ASCP) 2024 - TRANSLOCATIONS, DISEASES, PHARMA, POLYMERASES, & MORE 2025 BRAND NEW ACTUAL EXAM WITH ANSWERS.

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MB (ASCP) 2024 - TRANSLOCATIONS,
DISEASES, PHARMA, POLYMERASES, & MORE
2025 BRAND NEW ACTUAL EXAM WITH
ANSWERS.




How does capillary electrophoresis/primed PCR look for full
mutation? - correct answer -Continuous expanded peaks with
even gaps (?)




Southern Blot steps - correct answer -DGDBP
Digest/fragment
Gel electrophoresis
Denature
Blotting
Probe labeling




HLA Typing
Class I - correct answer -HLA-A
HLA-B

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HLA-C


exon 2 and 3




HLA Typing
Class II - correct answer -HLA-D


exon 2




What kind of bonds does denaturation destroy during southern
blot process? - correct answer -INTER-strand (means between
strands)


*also note: INTRA-strand means within strands




HCV genotype 1 - correct answer -Responds poorly to ribavirin
therapy




HCV genotype 2 - correct answer -Responds well to ribavirin
therapy

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Molecular Beacon design - correct answer -5' (Reporter) -
Sequence - 3' (Quencher)




In NGS, what is coverage referring to? - correct answer -#/times a
base is read




NGS steps - correct answer -Fragmentation
End-Repair
Adapter Ligation
QC Libraries




Number of SNPs in humans? - correct answer -10^7 (however
book says 5 million)




What cancer is FLT-3 involved with? - correct answer -AML




Reporter Probe Interference? - correct answer -5'G