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1. Which of the following statements about transcription factors are false?
Please select all that apply.
Each gene is controlled by multiple transcription factors
Binding sites are not known for all human transcription factors.
Each transcription factor is specific to one gene
A transcription factor binds to all instances of its recognition sequence:
Each transcription factor is specific to one gene
A transcription factor binds to all instances of its recognition sequence
2. True or false? Genes with a long 5' UTR are often subject to more
extensive transcriptional regulation than genes with a short 5'UTR.: False
(Genes with a long 5' untranslated region (UTR) are not regulated differently at the
transcriptional level, but they can be subject to extensive translational regulation.
This is mediated by proteins binding to the primary transcript in the 5' UTR region)
,3. Base changes in which of the following can have evolutionary
consequences? Please select all that apply.
CRM
Protein-coding gene
Promoter sequence
Non-coding RNA
mRNA: CRM
Protein-coding gene
Promoter Sequence
4. What is the role of the Mediator complex?: To interact between
transcription factors and RNA polymerase
5. A gene has acquired a mutation in which the protein product has an
"extra" 50 amino acids at the end. Which region of the gene was likely
mutated? start codon stop codon promoter sequence terminator sequence
coding region: stop codon
6. A eukaryotic DNA sequence that increases transcription at distant
promoters is called a(n): enhancer silencer repressor mediator insulator:
enhancer
, 7. A protein is known to be present in a specific sub-set of neurons in the
brain of the mouse. However, when the gene that codes for this protein is
mutated in such a way that it is rendered non-functional and the protein is
no longer produced in these neurons, the scientists studying this gene are
unable to detect any change in the appearance or function of these neurons.
Provide two possible explanations for this result.: there is a related gene in
the mouse genome capable of carrying out the same function; that is this gene is
one of a gene family the gene has no function in these cells. the gene codes for a
protein that has a function in these neurons but there is another biochemical
pathway in these cells that leads to the same function
8. A 90-residue peptide is found to be encoded by a 600 nucleotide gene
that contains no introns.
a) What are possible explanations for the small size of the peptide, relative
to the gene?
b) The mRNA transcribed from the gene is 680 nucleotides long. The RNA
contains 30% adenines, a much higher proportion than predicted from the
gene. What explains the large size of the mRNA relative to the gene?: a) If
there are no introns, we have to consider explanations that involve other steps in
gene expression. One possibility is that the mRNA has a long 5'UTR or 3'UTR.