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MLS (Medical Laboratory Scientist) ASCP Exam
Newest 2025 with accurate and verified questions
covering clinical chemistry, hematology, microbiology,
immunohematology, molecular diagnostics, laboratory
operations, and quality control. Designed to prepare
candidates for ASCP certification with up-to-date
clinical and procedural content.
Which of the following Rh antigens is found the highest
frequency in the Caucasian population:
A. C
B. E
C. c
D. e - ANSWER-D. e
The accuracy of an immunoassay is its ability to discriminate
between results that are true positives and results that are true
negatives. Two parameters of test accuracy are specificity and
sensitivity. Which of these statements apply to an immunoassay
with high specificity, but low sensitivity? - ANSWER-
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Accurately identifies the absence of disease and has few false-
positives
A test with high specificity accurately detects the absence of
disease. The more specific a test is, the fewer false-positive
results will occur. A test with high sensitivity accurately
identifies the presence of disease. The more sensitive a test, the
fewer false-negative results it produces. In the case stated in this
question, the immunoassay has high specificity, so it has few
false-positives and will accurately detect those individuals who
do not have the disease or condition that is being tested for.
However, the test has low sensitivity, so it may not identify all
individuals who actually have the disease; it may produce many
false-negative results.
Distinguishing characteristics of Micrococcus luteus: -
ANSWER-Resistance to furazolidone is one of the key
characteristics by which members of the genus Micrococcus
(resistant) can be separated from members of the genus
Staphylococcus (susceptible). Micrococcus species also are
susceptible to novobiocin and to bacitracin, although the zone of
inhibition to the "A" disk is often small (as shown in this
photograph).
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The sickling phenomenon observed in erythrocytes containing
hemoglobin S may be induced by which of the following? -
ANSWER-Reducing agents actually induce the sickling
phenomenon in RBCs when they contain hemoglobin S.
If an autologous blood donor weighs 35 kg, how much blood
can be collected for later transfusion? - ANSWER-315 mL
Volume to draw = (Donor's weight in kilograms / 50 kg
(minimum weight requirement) x 450 mL (volume of unit to be
donated)
In this case, Volume to draw = (35 kg / 50 kg) x 450 mL = 315
mL
Which adipokine is decreased in obesity, is anti-inflammatory,
and increases insulin sensitivity? - ANSWER-Adiponectin is a
protective cytokine. This cytokine is anti-inflammatory and
increases insulin sensitivity. Adiponectin is usually decreased in
obesity.
Distinguishing characteristics of Mycobacterium fortuitum: -
ANSWER-Mycobacterium fortuitum is positive for iron uptake,
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nitrate, arysulfatase, and growth on MacConkey agar without
crystal violet in five days.
CD5 antigen is normally found on which of the following
lymphocyte populations? - ANSWER-Mature T cells
Define: Congestive heart failure - ANSWER-A left ventricular
dysfunction resulting from aging, hypertension, atherosclerosis
or muscle damage from an AMI or repeated AMIs.
Define infarction: - ANSWER-An area of tissue death that
occurs due to lack of oxygen.
Define: Ischemia - ANSWER-An inadequate blood supply that
decreases availability of oxygen.
Define: Angina - ANSWER-Chest pain caused by inadequate
supply of oxygen to heart myocardium.
Which of the following antigen groups is closely related to the
ABO antigens on the red cell membrane? - ANSWER-I and i
antigens are related biochemically and spatially to the ABH and
Lewis antigens on the red cell membrane. Anti-I is usually an
MLS (Medical Laboratory Scientist) ASCP Exam
Newest 2025 with accurate and verified questions
covering clinical chemistry, hematology, microbiology,
immunohematology, molecular diagnostics, laboratory
operations, and quality control. Designed to prepare
candidates for ASCP certification with up-to-date
clinical and procedural content.
Which of the following Rh antigens is found the highest
frequency in the Caucasian population:
A. C
B. E
C. c
D. e - ANSWER-D. e
The accuracy of an immunoassay is its ability to discriminate
between results that are true positives and results that are true
negatives. Two parameters of test accuracy are specificity and
sensitivity. Which of these statements apply to an immunoassay
with high specificity, but low sensitivity? - ANSWER-
,2|Page
Accurately identifies the absence of disease and has few false-
positives
A test with high specificity accurately detects the absence of
disease. The more specific a test is, the fewer false-positive
results will occur. A test with high sensitivity accurately
identifies the presence of disease. The more sensitive a test, the
fewer false-negative results it produces. In the case stated in this
question, the immunoassay has high specificity, so it has few
false-positives and will accurately detect those individuals who
do not have the disease or condition that is being tested for.
However, the test has low sensitivity, so it may not identify all
individuals who actually have the disease; it may produce many
false-negative results.
Distinguishing characteristics of Micrococcus luteus: -
ANSWER-Resistance to furazolidone is one of the key
characteristics by which members of the genus Micrococcus
(resistant) can be separated from members of the genus
Staphylococcus (susceptible). Micrococcus species also are
susceptible to novobiocin and to bacitracin, although the zone of
inhibition to the "A" disk is often small (as shown in this
photograph).
,3|Page
The sickling phenomenon observed in erythrocytes containing
hemoglobin S may be induced by which of the following? -
ANSWER-Reducing agents actually induce the sickling
phenomenon in RBCs when they contain hemoglobin S.
If an autologous blood donor weighs 35 kg, how much blood
can be collected for later transfusion? - ANSWER-315 mL
Volume to draw = (Donor's weight in kilograms / 50 kg
(minimum weight requirement) x 450 mL (volume of unit to be
donated)
In this case, Volume to draw = (35 kg / 50 kg) x 450 mL = 315
mL
Which adipokine is decreased in obesity, is anti-inflammatory,
and increases insulin sensitivity? - ANSWER-Adiponectin is a
protective cytokine. This cytokine is anti-inflammatory and
increases insulin sensitivity. Adiponectin is usually decreased in
obesity.
Distinguishing characteristics of Mycobacterium fortuitum: -
ANSWER-Mycobacterium fortuitum is positive for iron uptake,
, 4|Page
nitrate, arysulfatase, and growth on MacConkey agar without
crystal violet in five days.
CD5 antigen is normally found on which of the following
lymphocyte populations? - ANSWER-Mature T cells
Define: Congestive heart failure - ANSWER-A left ventricular
dysfunction resulting from aging, hypertension, atherosclerosis
or muscle damage from an AMI or repeated AMIs.
Define infarction: - ANSWER-An area of tissue death that
occurs due to lack of oxygen.
Define: Ischemia - ANSWER-An inadequate blood supply that
decreases availability of oxygen.
Define: Angina - ANSWER-Chest pain caused by inadequate
supply of oxygen to heart myocardium.
Which of the following antigen groups is closely related to the
ABO antigens on the red cell membrane? - ANSWER-I and i
antigens are related biochemically and spatially to the ABH and
Lewis antigens on the red cell membrane. Anti-I is usually an
