Bio 181 Lab Final Questions With Complete
Solutions
How are enzymes regulated in the body? Correct
Answers Optimal temp: 35-40
Optimal pH: 6-8
Feedback inhibition regulates what enzymes are in
use and if they are needed
How are fragments separated in gel
electrophoresis? Correct Answers By their size,
small molecules will move through the gel quicker
than big ones
How do enzymes catalyze reactions? Correct
Answers By forming a substrate-enzyme complex.
The enzyme brings the substrates together and put
stress to break the bonds. Enzymes create a micro-
environment or break bonds
How does an inhibitor work? Correct Answers
Prevents binding of the target molecule of the
enzyme by binding to the active site or another
site
How does DNA move through gel electrophoresis?
Correct Answers Since they are negatively
charged, they move towards the anodes
,How does pH affect enzymatic activity? Correct
Answers Enzymes function in a certain pH, if the
pH changes more acidic or basic the enzyme's
functional groups change. This changes the
structure. Extreme pH can denature enzymes by
breaking the hydrogen bonds between them
How does substrate specificity affect enzymatic
activity? Correct Answers Substrate specificity is a
term for the fact that each enzyme generally
converts only one specific substrate for one
specific reaction. The more specific a substrate is,
the enzyme will want to react with that substrate
How does substrate/enzyme concentration affect
enzymatic activity? Correct Answers If there is too
much substrate compared to the enzyme,
enzymatic activity will plateau
Increasing the amount of enzymes will increase the
rate of enzymatic activity
How does temperature affect enzymatic activity?
Correct Answers Enzymes function in an optimal
temperature, if the temperature changes their
function changes. Extreme high temperatures or
cold temperatures denature an enzymes. Some
cold temperatures the enzyme can renature
How many diploid combinations is there? Correct
Answers 70 billion
, Meiosis 1 is known for? What about Meiosis 2
Correct Answers Separation of homologous
chromosomes; separation of sister chromatids
Review DNA Replication Quizlet Correct Answers
Review DNA transcription/translation Quizlet
Correct Answers
What 3 things contribute to genetic variation?
Correct Answers Independent assortment, crossing
over, random fertilization
What affects enzymatic activity? Correct Answers
Substrate specificity, substrate/enzyme
concentration, pH and temperature
What affects gel electrophoresis? Correct Answers
SIZE AND SHAPE OF THE MOLECULE
INTENSITY OF THE ELECTRIC CURRENT
AGAROSE GEL CONCENTRATION
CHARGE OF THE MOLECULES
CONDITION OF THE BUFFER (PH, TEMP)
Solutions
How are enzymes regulated in the body? Correct
Answers Optimal temp: 35-40
Optimal pH: 6-8
Feedback inhibition regulates what enzymes are in
use and if they are needed
How are fragments separated in gel
electrophoresis? Correct Answers By their size,
small molecules will move through the gel quicker
than big ones
How do enzymes catalyze reactions? Correct
Answers By forming a substrate-enzyme complex.
The enzyme brings the substrates together and put
stress to break the bonds. Enzymes create a micro-
environment or break bonds
How does an inhibitor work? Correct Answers
Prevents binding of the target molecule of the
enzyme by binding to the active site or another
site
How does DNA move through gel electrophoresis?
Correct Answers Since they are negatively
charged, they move towards the anodes
,How does pH affect enzymatic activity? Correct
Answers Enzymes function in a certain pH, if the
pH changes more acidic or basic the enzyme's
functional groups change. This changes the
structure. Extreme pH can denature enzymes by
breaking the hydrogen bonds between them
How does substrate specificity affect enzymatic
activity? Correct Answers Substrate specificity is a
term for the fact that each enzyme generally
converts only one specific substrate for one
specific reaction. The more specific a substrate is,
the enzyme will want to react with that substrate
How does substrate/enzyme concentration affect
enzymatic activity? Correct Answers If there is too
much substrate compared to the enzyme,
enzymatic activity will plateau
Increasing the amount of enzymes will increase the
rate of enzymatic activity
How does temperature affect enzymatic activity?
Correct Answers Enzymes function in an optimal
temperature, if the temperature changes their
function changes. Extreme high temperatures or
cold temperatures denature an enzymes. Some
cold temperatures the enzyme can renature
How many diploid combinations is there? Correct
Answers 70 billion
, Meiosis 1 is known for? What about Meiosis 2
Correct Answers Separation of homologous
chromosomes; separation of sister chromatids
Review DNA Replication Quizlet Correct Answers
Review DNA transcription/translation Quizlet
Correct Answers
What 3 things contribute to genetic variation?
Correct Answers Independent assortment, crossing
over, random fertilization
What affects enzymatic activity? Correct Answers
Substrate specificity, substrate/enzyme
concentration, pH and temperature
What affects gel electrophoresis? Correct Answers
SIZE AND SHAPE OF THE MOLECULE
INTENSITY OF THE ELECTRIC CURRENT
AGAROSE GEL CONCENTRATION
CHARGE OF THE MOLECULES
CONDITION OF THE BUFFER (PH, TEMP)
