ASCP MB III: Laboratory Operations Practice Test Questions And Answers

ASCP MB III: Laboratory Operations Practice Test Questions And Answers blood and bone marrow specimens - ANSWER - ship overnight at room temperature or with ice packs isolated RNA - ANSWER - storage at room or fridge temp no recommended without stabilization - can be stored in ethanol at -20C for several months - long term storage is best in ethanol at -70C clinical sensitivity of an assay - ANSWER TP/(TP+FN) - lowest level detected at least 95% of the time clinical specificity of an assay - ANSWER TN/(TN+FP) - negative samples are always negative and positive results are true positives (TP) accuracy of an assay - ANSWER (TN+TP)/(TN+TP+FN+FP) X 100 TN = true negative discontinued SOPs are kept for at least _____ - ANSWER two years sensitivity control - ANSWER defines the lower limit of detection for more meaningful interpretation of negative results amplification control - ANSWER a target that should always amplify - used to distinguish true negative amplification results from false negative resulting from amplification failure high positive, low positive and negative controls - ANSWER are included in each run for quantitative methods internal controls - ANSWER - run in the same reaction mix as the test specimen - used in methods requiring detection of a target-specific product, or relative amounts of target - in PCR distinguishes false-negative results from failed amplifications quantitative results - ANSWER - should be within the linear range of the assay standard thermal cyclers - ANSWER decline in temperature control over time automatic pipetters - ANSWER - checked for accuracy every 6 mo using gravimetric methods (weighing specific volume of water) capillary systems - ANSWER clean buffer and polymer delivery channels and replace polymer at least twice per month annually - ANSWER - SOPs are reviewed - test fume hoods and laminar flow hoods (or if hood is moved or installed) - test spectrophotometers used to measure DNA, RNA, protein concentrations, and colorimetric assays and turbidity - test electrophoresis power supplies - test centrifuges and microcentrifuges (using a tachometer) types of probes - ANSWER genomic, cDNA, oligonucleotide, plasmid, or riboprobe analyte specific reagents (ASR) - ANSWER probes, primers, antibodies, and other test components that detect a specific target, such as a cell surface protein or DNA mutation NFPA hazard diamond - ANSWER red - flammability blue - health hazard yellow - stability white - special hazards O (oxidizer) , W (dangerous reactivity to water) proficiency testing - ANSWER analysis of external specimens from a reference source supplied to independent laboratories - used to asses skills of personnel acrylic shielding - ANSWER required for working with gamma emitters, such as 32^P and 33^P in situ results (eg FISH) - ANSWER documentation should include images of at least one normal cell with at least two abnormal cell results resequencing - ANSWER repeated sequencing across the same area for known sequence changes - s.t. performed on only one template strand; both strands is best test results are not released to the following parties without the patient's expressed consent: - ANSWER employers, insurers, family members an unlabled collection tube with a requisition for a factor V Leiden test is received in the laboratory - ANSWER notify the supervisor and reject the specimen after PCR, the amplification control has failed to yield a product - ANSWER check the original DNA or RNA preparation. if it is inadequate, repeat the amplification. if not, reisolate the nucleic acid An isolated DNA sample is to be stored for at least 6 months. - ANSWER store at -70C in a tightly sealed tube a bone marrow specimen arrives at the end of a shift and will not be processed for the Bc12 translocation until the next day - ANSWER place the specimen in the refrigerator the temperature of a refrigerator set at 8°C (±2°C) reads 14°C. - ANSWER recheck the temperature after a few hours. If it does not return to range, notify the supervisor. A PCR test for the BCR/ABL translocation was negative for the patient sample and for the sensitivity control. - ANSWER repeat the PCR with the addition of a new sensitivity control. A fragile X test result has been properly reviewed and reported - ANSWER file the test results, documents, and associated autoradiographs together in the laboratory archives A bottle of reagent alcohol with a 3 in the red diamond on its label is to be stored. - ANSWER in a safety storage cabinet for flammable liquids expiration date on a reagent is passed. - ANSWER Discard the reagent. If it can be used for research or other nonclinical purposes, label and store it in a separate area away from patient testing reagents. test results are to be faxed to the ordering physician - ANSWER fax the results with a cover sheet containing the proper disclaimer limit of detection - ANSWER the lowest viral concentration that can be detected 95% of the time in the test assay research use only (RUO) - ANSWER - a given product is for research such as basic laboratory research, performance investigation, design investigation, etc.