MIP 300 Final exam questions with 100% correct
answers | Verified
/who provides the lipids in a viral envelope - Answer-the cell
/.all viruses have a protein coat - Answer-true
/.a virulent phage can inadvertently package host cell chromosome into phage particles
- Answer-true
/.you have a bacterium that picks up a new gene by Hfr conjugation. what is an
explanation of why progeny of this bacterium express this trait? - Answer-the gene is
integrated into the genome
/.after successful transformation of a fragment of chromosomal DNA, the recipient
(female) will become a donor (male) capable of creating a sex pili to transfer DNA -
Answer-false
/.you isolate a virulent page. It can perform specialized transduction - Answer-false
/.you want to know if a baby still has vaccine neutralizing antibody that it received from
its mom in utero. Which of the following types of ELISAs would you run? one that tests
for - Answer-IgG
/.in differential centrifugation, virus is first centrifuged at a slow speed, the supernatant
is removed and re-centrifuged at a very fast speed - Answer-true
/.Virus stocks prepared by differential centrifugation and gradient centrifugation are
equally clean - Answer-false
/.hemagglutination assay - Answer-determines highest dilution of virus that causes red
blood cells to clump together
/.cytopathic effect - Answer-a visible effect on a host cell, caused by a virus, that may
result in host cell damage or death
/.immuno fluorescence assay - Answer-primarily on biological samples and is classically
defined as a procedure to detect antigens in cellular contexts using antibodies.
/.plaque assay - Answer-method used to measure the number of viral particles present
in a sample
, /.a temperate phage can inadvertently package host cell chromosome into phage
particles - Answer-true
/.you isolate a bacterium that can transfer genes to other bacteria only when it is in
direct contact with these bacteria. it is most likely transferring the genes by - Answer-
conjugation
/.a piece of host cell dna bound to a piece of phage dna after excising from the
chromosome. when this phage that contains host cell dna infects a new cell, will the
new cell be killed by this infection? - Answer-no
/.after successful generalized transduction, the recipient (female) will become a donor
(male) capable of creating a sex pili to transfer dna - Answer-false
/.can a temperate phage perform generalized and specialized transduction? - Answer-
yes
/.what kind of organism can carry out the conversion of O2 --> H2O? - Answer-aerobic
respiration and aerobic chemolithotrophy
/.an error in dna replication causes a mutation changing AGG to CGG, what change do
you expect? - Answer-no change to protein function
/.the organism with the more positive reduction potential will produce more energy -
Answer-true
/.dna ligase forms - Answer-the last phosphodiester bond between dna single strands
/.dna ligase forms hydrogen bonds - Answer-false
/.for each acetyl-coA that enters the TCA cycle, how many ATP are generayed by
substrate level phosphorylation - Answer-1
/.you correctly perform a gran stain on bacteria that contain LPS, what color will you see
on the slide? - Answer-pink
/.a bacterium contains 2 carbohydrates and 5 different amino acids in its cell wall. What
color will it gram stain? - Answer-purple
/.an antibiotic inhibits ribosome function and prevents bacteria from sporulating. which
step in sporulation is affected - Answer-formation of spore coats
/.a bacterium is known to be a psychophile. which of the following would be true for this
organism? - Answer-generation time shortest at 0-20 degrees celcsius and growth rate
the fastest
answers | Verified
/who provides the lipids in a viral envelope - Answer-the cell
/.all viruses have a protein coat - Answer-true
/.a virulent phage can inadvertently package host cell chromosome into phage particles
- Answer-true
/.you have a bacterium that picks up a new gene by Hfr conjugation. what is an
explanation of why progeny of this bacterium express this trait? - Answer-the gene is
integrated into the genome
/.after successful transformation of a fragment of chromosomal DNA, the recipient
(female) will become a donor (male) capable of creating a sex pili to transfer DNA -
Answer-false
/.you isolate a virulent page. It can perform specialized transduction - Answer-false
/.you want to know if a baby still has vaccine neutralizing antibody that it received from
its mom in utero. Which of the following types of ELISAs would you run? one that tests
for - Answer-IgG
/.in differential centrifugation, virus is first centrifuged at a slow speed, the supernatant
is removed and re-centrifuged at a very fast speed - Answer-true
/.Virus stocks prepared by differential centrifugation and gradient centrifugation are
equally clean - Answer-false
/.hemagglutination assay - Answer-determines highest dilution of virus that causes red
blood cells to clump together
/.cytopathic effect - Answer-a visible effect on a host cell, caused by a virus, that may
result in host cell damage or death
/.immuno fluorescence assay - Answer-primarily on biological samples and is classically
defined as a procedure to detect antigens in cellular contexts using antibodies.
/.plaque assay - Answer-method used to measure the number of viral particles present
in a sample
, /.a temperate phage can inadvertently package host cell chromosome into phage
particles - Answer-true
/.you isolate a bacterium that can transfer genes to other bacteria only when it is in
direct contact with these bacteria. it is most likely transferring the genes by - Answer-
conjugation
/.a piece of host cell dna bound to a piece of phage dna after excising from the
chromosome. when this phage that contains host cell dna infects a new cell, will the
new cell be killed by this infection? - Answer-no
/.after successful generalized transduction, the recipient (female) will become a donor
(male) capable of creating a sex pili to transfer dna - Answer-false
/.can a temperate phage perform generalized and specialized transduction? - Answer-
yes
/.what kind of organism can carry out the conversion of O2 --> H2O? - Answer-aerobic
respiration and aerobic chemolithotrophy
/.an error in dna replication causes a mutation changing AGG to CGG, what change do
you expect? - Answer-no change to protein function
/.the organism with the more positive reduction potential will produce more energy -
Answer-true
/.dna ligase forms - Answer-the last phosphodiester bond between dna single strands
/.dna ligase forms hydrogen bonds - Answer-false
/.for each acetyl-coA that enters the TCA cycle, how many ATP are generayed by
substrate level phosphorylation - Answer-1
/.you correctly perform a gran stain on bacteria that contain LPS, what color will you see
on the slide? - Answer-pink
/.a bacterium contains 2 carbohydrates and 5 different amino acids in its cell wall. What
color will it gram stain? - Answer-purple
/.an antibiotic inhibits ribosome function and prevents bacteria from sporulating. which
step in sporulation is affected - Answer-formation of spore coats
/.a bacterium is known to be a psychophile. which of the following would be true for this
organism? - Answer-generation time shortest at 0-20 degrees celcsius and growth rate
the fastest
