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ia.com/
,Chapter-1-RBCs-and-Platelet-Preservation:-Historical-Perspectives-and-Current-Trends
Multiple-Choice
Identify-the-choice-that-best-completes-the-statement-or-answers-the-question.
1. Which-metabolic-pathway-is-responsible-for-generating-90%-of-the-ATP-for-the-RBC?
a. Pentose-phosphate-shunt c. Glycolysis
b. Luebering-Rapoport-shunt d.- Methemoglobin-reductase
2. A-unit-of-blood-was-returned-to-the-blood-bank-before-it-was-spiked.-Apparently-the-patient’s-IV-failed.-The-
unit-of-blood-was-outside-the-blood-bank-for-35-minutes.-Which-of-the-statements-below-is-most-accurate?
a. The-unit-of-blood-should-be-discarded-immediately.
b. The-unit-of-blood-can-be-returned-to-inventory.
c. The-unit-of-blood-must-be-transfused-within-4-hours-or-be-discarded-at-the-end-of-that-time.
d. The-unit-of-blood-must-be-transfused-with-24-hours.
3. What-is-the-average-diameter-of-a-platelet?
a. 5-to-10-µm c. 1-to-2-µm
b. 2-to-4-µm d.- - 4-to-6-µm
4. In-the-normal-hemoglobin-oxygen-dissociation-curve,-what-percentage-of-oxygen-is-released-to-the-tissues-
when-PO2- averages-40-mm-Hg?
a. 75% c. 100%
b. 25% d.- 50%
5. What-factors-are-known-to-influence-platelet-metabolism-and-function?
a. Storage-temperature c. Platelet-count
b. Initial-pH d.- All-of-the-above
6. Which-of-the-following-red-blood-cell-morphologies-may-be-present-on-the-peripheral-blood-smear-as-a-result-
of-loss-of-RBC-membrane?
a. Spherocytes c. Burr-cells
b. Target-cells d.- Schistocytes
7. What-does-the-term-autologous-transfusion-refer-to?
a. A-parent-donating-blood-for-his-or-her-child
b. An-individual-donating-blood-for-a-friend
c. An-individual-donating-blood-for-a-relative
d. An-individual-donating-blood-for-his-or-her-own-transfusion
8. What-is-the-primary-function-of-hemoglobin?
a. Iron-metabolism c. Oxygen-transport
b. Porphyrin-synthesis d.- Signal-transduction
9. All-of-the-following-areas-of-red-blood-cell-biology-are-crucial-for-normal-erythrocyte-survival-except:
a. Cellular-metabolism. c. Site-of-the-ABO-antigen-attachment.
b. RBC-membrane. d.- Hemoglobin-structure.
,- 10.- What-is-the-correct-biochemical-composition-of-the-RBC-membrane?
a. 52%-protein,-40%-lipid,-8%-carbohydrate
b. 40%-protein,-8%-lipid,-52%-carbohydrate
c. 8%-protein,-52%-lipid,-40%-carbohydrate
d. 8%-lipid,-40%-carbohydrate,-52%-protein
- 11.- All-of-the-following-biochemical-changes-are-associated-with-loss-of-red-blood-cell-viability-upon-storage
except:
a. Decreased-pH. c. Increased-ATP-level.
b. Loss-of-red-blood-cell-function. d.- Decreased-glucose-consumption.
- 12.- Which-red-blood-cell-preservative-has-a-storage-time-of-35-days?
a. ACD c. AS-1
b. CPDA-1 d.- - CPD
- 13.- The-RBC-membrane-is-relatively-permeable-to-all-of-the-following-except:
a. Chloride. c. Bicarbonate.
b. Sodium. d.- Water.
- 14.-What-percentage-of-platelets-is-sequestered-in-the-spleen-as-functional-reserve-after-being-released-from-the-
bone-marrow?
a. 30% c. 10%
b. 50% d.- - 25%
- 15.- What-is-the-major-biochemical-consideration-in-platelet-storage?
a. Glucose-metabolism c. Production-of-carbon-dioxide
b. Oxygen-supply d.- Regulation-of-pH
- 16.-What-would-the-hemoglobin-oxygen-dissociation-curve-depict-in-a-patient-exhibiting-clinical-signs-of-
alkalosis?
a. Normal c. Shift-to-the-right
b. Shift-to-the-left d.- None-of-the-above
- 17.- Name-the-main-lipid-components-of-a-red-blood-cell-membrane.
a. Phospholipid c. Glycolipid
b. Sphingomyelin d.- Glycophorin-A
- 18.- The-ABO-blood-groups-were-discovered-in-1901-by-whom?
a. Charles-Drew c. Loutit-and-Mollison
b. Karl-Landsteiner d.- Edward-Lindeman
- 19.-A-standing-order-of-platelets-was-shipped-to-your-facility-by-your-supplier.-It-was-inadvertently-left-in-the-
corner-of-the-department-until-discovered-36-hours-later.-What-would-the-appropriate-action-be-for-the-blood-
banker?
a. If-the-temperature-in-the-box-was-22-+/--2°C-and-the-platelet-swirl-seemed-OK,-it-would-be-
OK-to-accept-the-unit-into-inventory.
b. The-platelets-have-fallen-outside-the-supplier’s-quality-assurance.-The-unit-should-be-
discarded-because-the-pH-has-probably-dropped-too-low-and-platelet-activation-has-been-
compromised.
c. If-the-temperature-was-1°C-to-6°C-and-the-platelet-swirl-seemed-OK,-it-would-be-OK-to
, accept-the-unit-into-inventory.
d. If-the-platelets-appeared-OK-and-passed-the-platelet-swirl-test-after-being-placed-on-the-
agitator,-they-could-be-accepted-into-the-inventory.
- 20.- Which-metabolic-pathway-permits-the-accumulation-of-2,3-diphosphoglycerate-(2,3-DPG)?
a. Glycolysis c. Pentose-phosphate-shunt
b. Luebering-Rapoport-shunt d.- Methemoglobin-reductase
- 21.- All-of-the-following-are-consistent-with-a-"shift-to-the-right"-of-the-hemoglobin-oxygen-dissociation-curve
except:
a. Increased-2,3-DPG.
b. 50%-O2- saturation-to-tissues.
c. Decreased-2,3-DPG.
d. Decreased-hemoglobin-affinity-for-O2.
- 22.- What-is-the-normal-platelet-count-range-per-cubic-millimeter?
a. 100-to-350 c. 50,000-to-200,000
b. 150,000-to-350,000 d.- 350,000-to-500,000
- 23.- What-cryoprotective-agent-is-added-to-red-blood-cells-upon-freezing?
a. Dextrose c. Glycerol
b. Adsol d.- All-of-the-above
- 24.- If-platelets-are-to-be-stored-for-5-days-on-a-rotator,-what-is-the-optimal-storage-temperature?
a. 1°C-to-6°C c. 35°C-to-37°C
b. 20°C-to-24°C d.- 1°C-to-10°C
- 25.- What-role-do-platelets-play-in-hemostasis?
a. Maintenance-of-vascular-integrity
b. Initial-arrest-of-bleeding-by-platelet-plug-formation
c. Stabilization-of-the-hemostatic-plug
d. All-of-the-above
- 26.- Which-of-the-following-best-describes-"integral"-membrane-proteins?
a. Reside-at-the-cytoplasmic-surface-of-membrane
b. Span-the-entire-membrane-surface
c. Form-the-red-blood-cell-cytoskeleton
d. None-of-the-above
- 27.- How-is-stroma-free-hemoglobin-solution-prepared?
a. Outdated-red-blood-cells-are-concentrated,-and-stroma-is-removed.
b. Outdated-red-blood-cells-are-diluted-with-saline,-and-stroma-is-removed.
c. Outdated-red-blood-cells-are-lysed,-and-stroma-is-removed.
d. None-of-the-above
- 28.- What-is-the-normal-life-span-of-an-RBC?
a. 100-days c. 120-hours
b. 120-days d.- 2-days
- 29.- Regarding-loss-of-RBC-membrane-deformability,-all-of-the-following-are-true-except:
a. Increase-in-ATP-level.
b. Decrease-in-ATP-level.
ia.com/
,Chapter-1-RBCs-and-Platelet-Preservation:-Historical-Perspectives-and-Current-Trends
Multiple-Choice
Identify-the-choice-that-best-completes-the-statement-or-answers-the-question.
1. Which-metabolic-pathway-is-responsible-for-generating-90%-of-the-ATP-for-the-RBC?
a. Pentose-phosphate-shunt c. Glycolysis
b. Luebering-Rapoport-shunt d.- Methemoglobin-reductase
2. A-unit-of-blood-was-returned-to-the-blood-bank-before-it-was-spiked.-Apparently-the-patient’s-IV-failed.-The-
unit-of-blood-was-outside-the-blood-bank-for-35-minutes.-Which-of-the-statements-below-is-most-accurate?
a. The-unit-of-blood-should-be-discarded-immediately.
b. The-unit-of-blood-can-be-returned-to-inventory.
c. The-unit-of-blood-must-be-transfused-within-4-hours-or-be-discarded-at-the-end-of-that-time.
d. The-unit-of-blood-must-be-transfused-with-24-hours.
3. What-is-the-average-diameter-of-a-platelet?
a. 5-to-10-µm c. 1-to-2-µm
b. 2-to-4-µm d.- - 4-to-6-µm
4. In-the-normal-hemoglobin-oxygen-dissociation-curve,-what-percentage-of-oxygen-is-released-to-the-tissues-
when-PO2- averages-40-mm-Hg?
a. 75% c. 100%
b. 25% d.- 50%
5. What-factors-are-known-to-influence-platelet-metabolism-and-function?
a. Storage-temperature c. Platelet-count
b. Initial-pH d.- All-of-the-above
6. Which-of-the-following-red-blood-cell-morphologies-may-be-present-on-the-peripheral-blood-smear-as-a-result-
of-loss-of-RBC-membrane?
a. Spherocytes c. Burr-cells
b. Target-cells d.- Schistocytes
7. What-does-the-term-autologous-transfusion-refer-to?
a. A-parent-donating-blood-for-his-or-her-child
b. An-individual-donating-blood-for-a-friend
c. An-individual-donating-blood-for-a-relative
d. An-individual-donating-blood-for-his-or-her-own-transfusion
8. What-is-the-primary-function-of-hemoglobin?
a. Iron-metabolism c. Oxygen-transport
b. Porphyrin-synthesis d.- Signal-transduction
9. All-of-the-following-areas-of-red-blood-cell-biology-are-crucial-for-normal-erythrocyte-survival-except:
a. Cellular-metabolism. c. Site-of-the-ABO-antigen-attachment.
b. RBC-membrane. d.- Hemoglobin-structure.
,- 10.- What-is-the-correct-biochemical-composition-of-the-RBC-membrane?
a. 52%-protein,-40%-lipid,-8%-carbohydrate
b. 40%-protein,-8%-lipid,-52%-carbohydrate
c. 8%-protein,-52%-lipid,-40%-carbohydrate
d. 8%-lipid,-40%-carbohydrate,-52%-protein
- 11.- All-of-the-following-biochemical-changes-are-associated-with-loss-of-red-blood-cell-viability-upon-storage
except:
a. Decreased-pH. c. Increased-ATP-level.
b. Loss-of-red-blood-cell-function. d.- Decreased-glucose-consumption.
- 12.- Which-red-blood-cell-preservative-has-a-storage-time-of-35-days?
a. ACD c. AS-1
b. CPDA-1 d.- - CPD
- 13.- The-RBC-membrane-is-relatively-permeable-to-all-of-the-following-except:
a. Chloride. c. Bicarbonate.
b. Sodium. d.- Water.
- 14.-What-percentage-of-platelets-is-sequestered-in-the-spleen-as-functional-reserve-after-being-released-from-the-
bone-marrow?
a. 30% c. 10%
b. 50% d.- - 25%
- 15.- What-is-the-major-biochemical-consideration-in-platelet-storage?
a. Glucose-metabolism c. Production-of-carbon-dioxide
b. Oxygen-supply d.- Regulation-of-pH
- 16.-What-would-the-hemoglobin-oxygen-dissociation-curve-depict-in-a-patient-exhibiting-clinical-signs-of-
alkalosis?
a. Normal c. Shift-to-the-right
b. Shift-to-the-left d.- None-of-the-above
- 17.- Name-the-main-lipid-components-of-a-red-blood-cell-membrane.
a. Phospholipid c. Glycolipid
b. Sphingomyelin d.- Glycophorin-A
- 18.- The-ABO-blood-groups-were-discovered-in-1901-by-whom?
a. Charles-Drew c. Loutit-and-Mollison
b. Karl-Landsteiner d.- Edward-Lindeman
- 19.-A-standing-order-of-platelets-was-shipped-to-your-facility-by-your-supplier.-It-was-inadvertently-left-in-the-
corner-of-the-department-until-discovered-36-hours-later.-What-would-the-appropriate-action-be-for-the-blood-
banker?
a. If-the-temperature-in-the-box-was-22-+/--2°C-and-the-platelet-swirl-seemed-OK,-it-would-be-
OK-to-accept-the-unit-into-inventory.
b. The-platelets-have-fallen-outside-the-supplier’s-quality-assurance.-The-unit-should-be-
discarded-because-the-pH-has-probably-dropped-too-low-and-platelet-activation-has-been-
compromised.
c. If-the-temperature-was-1°C-to-6°C-and-the-platelet-swirl-seemed-OK,-it-would-be-OK-to
, accept-the-unit-into-inventory.
d. If-the-platelets-appeared-OK-and-passed-the-platelet-swirl-test-after-being-placed-on-the-
agitator,-they-could-be-accepted-into-the-inventory.
- 20.- Which-metabolic-pathway-permits-the-accumulation-of-2,3-diphosphoglycerate-(2,3-DPG)?
a. Glycolysis c. Pentose-phosphate-shunt
b. Luebering-Rapoport-shunt d.- Methemoglobin-reductase
- 21.- All-of-the-following-are-consistent-with-a-"shift-to-the-right"-of-the-hemoglobin-oxygen-dissociation-curve
except:
a. Increased-2,3-DPG.
b. 50%-O2- saturation-to-tissues.
c. Decreased-2,3-DPG.
d. Decreased-hemoglobin-affinity-for-O2.
- 22.- What-is-the-normal-platelet-count-range-per-cubic-millimeter?
a. 100-to-350 c. 50,000-to-200,000
b. 150,000-to-350,000 d.- 350,000-to-500,000
- 23.- What-cryoprotective-agent-is-added-to-red-blood-cells-upon-freezing?
a. Dextrose c. Glycerol
b. Adsol d.- All-of-the-above
- 24.- If-platelets-are-to-be-stored-for-5-days-on-a-rotator,-what-is-the-optimal-storage-temperature?
a. 1°C-to-6°C c. 35°C-to-37°C
b. 20°C-to-24°C d.- 1°C-to-10°C
- 25.- What-role-do-platelets-play-in-hemostasis?
a. Maintenance-of-vascular-integrity
b. Initial-arrest-of-bleeding-by-platelet-plug-formation
c. Stabilization-of-the-hemostatic-plug
d. All-of-the-above
- 26.- Which-of-the-following-best-describes-"integral"-membrane-proteins?
a. Reside-at-the-cytoplasmic-surface-of-membrane
b. Span-the-entire-membrane-surface
c. Form-the-red-blood-cell-cytoskeleton
d. None-of-the-above
- 27.- How-is-stroma-free-hemoglobin-solution-prepared?
a. Outdated-red-blood-cells-are-concentrated,-and-stroma-is-removed.
b. Outdated-red-blood-cells-are-diluted-with-saline,-and-stroma-is-removed.
c. Outdated-red-blood-cells-are-lysed,-and-stroma-is-removed.
d. None-of-the-above
- 28.- What-is-the-normal-life-span-of-an-RBC?
a. 100-days c. 120-hours
b. 120-days d.- 2-days
- 29.- Regarding-loss-of-RBC-membrane-deformability,-all-of-the-following-are-true-except:
a. Increase-in-ATP-level.
b. Decrease-in-ATP-level.
