PROBIOTICS MCB6424 EXAM 3- QUESTIONS
WITH CORRECT ANSWERS 2025
microbiotavvsvmicrobiomev-vCORRECTvANSWERv-microbiotav-vorganisms
microbiomev-vorganismsv+vgenesv+vgenome
Thevgutvmicrobiotavcontainsvmorevthanv__vcells
morevthanv__vspeciesv-vCORRECTvANSWERv-200vtrillionvcells
>1000vspecies
Thevmicrobiomevcontainsv__xvthevcellsvinvthevhumanvbody
Thevgutvmicrobiomevisv__xvlargervthanvthevhumanvgenomev-vCORRECTvANSWERv-10xvcells
150xvgenome
Whovcamevupvwithvthevofficialvdefinitionvofvmicrobiota?v-vCORRECTvANSWERv-
LederbergvandvMcCray,v2001
Microbiotavdefinitionv-vCORRECTvANSWERv-
"Ecologicalvcommunityvofvcommensal,vsymbiotic,vandvpathogenicvmicroorganismsvwithinvavbodyvspacevorv
othervenvironment"
True/Falsevthevmicrobiomevisvgenerallyvnon-pathogenicvandvbeneficialvtovthevhostv-vCORRECTvANSWERv-
true
Transientvmicrobiotav-vCORRECTvANSWERv-
microbesvthatvarevpresentvforvvariousvperiodsvandvthenvdisappear
Althoughvthesevtypesvofvorganismsvmayvcolonizevavhostvandvreproducevbriefly,vthevdefensesvofvthevbodyvd
ovnotvallowvthemvtovbecomevpermanent.
3vtypesvofvSymbiosisv-vCORRECTvANSWERv-mutualism,vcommensalism,vparasitism
, Commensalismv-vCORRECTvANSWERv-
onevorganismvbenefitsvthevothervandvthevothervisvunaffectedv(ex:vmycobacteriavinvthevear)
mutualismv-vCORRECTvANSWERv-bothvorganismsvbenefitv(ex:vE.vcolivinvthevcolon,vsynthesizesvvitvK)
parasitismv-vCORRECTvANSWERv-onevbenefitsvatvthevexpensevofvthevotherv(ex:vdiseasevcausingvbacteria)
___vplaysvavfundamentalvrolevinvimmunevmaturationv-
vcompletevabsencevofvbacteriavinvthevgutvresultsvinv___vandvlowvlevelsvofv____v-vCORRECTvANSWERv-
mutualism
resultsvinvgut-associatedvmesentericvlymphvtissuev
lowvlevelsvIgA
Specificvfunctionsvofvthevmicrobiotav-vCORRECTvANSWERv-
sealvbodyvspaces,vmitigatevintestinalvpathogens,vmaintainvtissuevhomeostasis,vfacilitatevfermentationvofvdi
etaryvfiberv(pectin,vetc),vcriticalvenergyvyield,vmetabolicvendvproductsvandvtherapeuticvdrugvprocessing,vsig
nalingvamongvcellsvandvorganvsystemsv(butyratev-venergyvforvgutvepithelialvcells;vacetatevandvpropionatev-
vlipogenesisvand
methodsvtovstudyvthevmicrobiotav-vCORRECTvANSWERv-16SvrRNAvgenevsequencing-
basedvsurveysvandvshotgunvmetagenomicvapproach
16SvrRNAvgenevsequencing-basedvsurveysv-vCORRECTvANSWERv-
Genevcontainsvhighlyvconservedvregionsvandvvariablevregions
amplifyv16SvrRNAvgenesvusingvprimersvdirectedvatvconservedvregionsvbutvflankingvvariablevregions
alignvgenevsequences,vresolvevphylogeneticvrelationshipsvatvdifferentvdepths
Mostv16SvrRNAvsequencesvcomevfromvpreviouslyv____v-vCORRECTvANSWERv-undescribedvmicrobes
WITH CORRECT ANSWERS 2025
microbiotavvsvmicrobiomev-vCORRECTvANSWERv-microbiotav-vorganisms
microbiomev-vorganismsv+vgenesv+vgenome
Thevgutvmicrobiotavcontainsvmorevthanv__vcells
morevthanv__vspeciesv-vCORRECTvANSWERv-200vtrillionvcells
>1000vspecies
Thevmicrobiomevcontainsv__xvthevcellsvinvthevhumanvbody
Thevgutvmicrobiomevisv__xvlargervthanvthevhumanvgenomev-vCORRECTvANSWERv-10xvcells
150xvgenome
Whovcamevupvwithvthevofficialvdefinitionvofvmicrobiota?v-vCORRECTvANSWERv-
LederbergvandvMcCray,v2001
Microbiotavdefinitionv-vCORRECTvANSWERv-
"Ecologicalvcommunityvofvcommensal,vsymbiotic,vandvpathogenicvmicroorganismsvwithinvavbodyvspacevorv
othervenvironment"
True/Falsevthevmicrobiomevisvgenerallyvnon-pathogenicvandvbeneficialvtovthevhostv-vCORRECTvANSWERv-
true
Transientvmicrobiotav-vCORRECTvANSWERv-
microbesvthatvarevpresentvforvvariousvperiodsvandvthenvdisappear
Althoughvthesevtypesvofvorganismsvmayvcolonizevavhostvandvreproducevbriefly,vthevdefensesvofvthevbodyvd
ovnotvallowvthemvtovbecomevpermanent.
3vtypesvofvSymbiosisv-vCORRECTvANSWERv-mutualism,vcommensalism,vparasitism
, Commensalismv-vCORRECTvANSWERv-
onevorganismvbenefitsvthevothervandvthevothervisvunaffectedv(ex:vmycobacteriavinvthevear)
mutualismv-vCORRECTvANSWERv-bothvorganismsvbenefitv(ex:vE.vcolivinvthevcolon,vsynthesizesvvitvK)
parasitismv-vCORRECTvANSWERv-onevbenefitsvatvthevexpensevofvthevotherv(ex:vdiseasevcausingvbacteria)
___vplaysvavfundamentalvrolevinvimmunevmaturationv-
vcompletevabsencevofvbacteriavinvthevgutvresultsvinv___vandvlowvlevelsvofv____v-vCORRECTvANSWERv-
mutualism
resultsvinvgut-associatedvmesentericvlymphvtissuev
lowvlevelsvIgA
Specificvfunctionsvofvthevmicrobiotav-vCORRECTvANSWERv-
sealvbodyvspaces,vmitigatevintestinalvpathogens,vmaintainvtissuevhomeostasis,vfacilitatevfermentationvofvdi
etaryvfiberv(pectin,vetc),vcriticalvenergyvyield,vmetabolicvendvproductsvandvtherapeuticvdrugvprocessing,vsig
nalingvamongvcellsvandvorganvsystemsv(butyratev-venergyvforvgutvepithelialvcells;vacetatevandvpropionatev-
vlipogenesisvand
methodsvtovstudyvthevmicrobiotav-vCORRECTvANSWERv-16SvrRNAvgenevsequencing-
basedvsurveysvandvshotgunvmetagenomicvapproach
16SvrRNAvgenevsequencing-basedvsurveysv-vCORRECTvANSWERv-
Genevcontainsvhighlyvconservedvregionsvandvvariablevregions
amplifyv16SvrRNAvgenesvusingvprimersvdirectedvatvconservedvregionsvbutvflankingvvariablevregions
alignvgenevsequences,vresolvevphylogeneticvrelationshipsvatvdifferentvdepths
Mostv16SvrRNAvsequencesvcomevfromvpreviouslyv____v-vCORRECTvANSWERv-undescribedvmicrobes