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Revision Examination Tests
“Come all for this Greatness”
... 100% Correct Ans ...
BOC MICROBIOLOGY 6TH EDITION EXAM 1 2024-2025
QUESTIONS AND CORRECT DETAILED ANSWERS
1. The proper blood-to-broth ratio for blood cultures to reduce the antibacterial effect
of serum in adults is:
a. 1:2
b. 1:3
c. 1:10
d. 1:30
Ans: 1:10
Quiz 2. The most appropriate method for collecting a urine specimen from a patient
with an indwelling catheter is:
a. remove the catheter, cut the tip, and submit it for culture
b. disconnect the catheter from the bag, and aseptically collect urine from the
terminal end of the catheter
c. aseptically collect urine directly from the drainage bag
d. aspirate urine aseptically from the catheter tubing
Ans: aspirate urine aseptically from the catheter tubing
Quiz 3. Which of the following groups of specimens would be acceptable for
anaerobic culture?
a. vaginal swab, eye swab
b. intraoral surface swab, leg tissue
c. pleural fluid, brain abscess fluid
d. urine, sputum
Ans: pleural fluid, brain abscess fluid
Quiz 4. Sodium polyanetholsulfonate (SPS) is used as an anticoagulant for blood
cultures because it:
a. inactivates penicillin and cephalosporins
b. prevents clumping of red cells
c. inactivates neutrophils and components of serum complement
d. facilitates growth of anaerobes
,Ans: inactivates neutrophils and components of serum complement
Quiz 5. The optimal collection of a wound specimen for culture of anaerobic
organisms is a:
a. swab of lesion obtained before administration of antibiotics
b. swab of lesion obtained after administration of antibiotics
c. syringe filled with pus, obtained before administration of antibiotics
d. syringe filled with pus, obtained after administration of antibiotics
Ans: syringe filled with pus, obtained before administration of antibiotics
Quiz 6. The most important variable in the recovery of organisms in adult patients
with bacteremia (bacterial sepsis) is:
a. subculture of all bottles at day 5 of incubation
b. the recommended volume of blood cultured
c. collection of daily blood culture sets for 3 consecutive days
d. collection of multiple blood culture sets from a single venipuncture
Ans: the recommended volume of blood cultured
Quiz 7. Virus transport medium should contain agents that:
a. enable rapid viral growth during the transport time
b. inhibit bacterial and fungal growth
c. destroy nonpathogenic viruses
d. inhibit complement-fixing antibodies
Ans: inhibit bacterial and fungal growth
Quiz 8. A bronchoscopy sample with the request for culture of Legionella is sent to
the laboratory. The correct plating protocol is:
a. culture on thiosulfate citrate bile salt media
b. incubate the culture media anaerobically
c. reject the specimen and request a sputum sample
d. culture on buffered charcoal yeast extract agar with antibiotics
Ans: culture on buffered charcoal yeast extract agar with antibiotics
Quiz 9. A community hospital microbiology laboratory is processing significant
numbers of stool cultures because of an outbreak of diarrhea following heavy rains
and flooding in the county. A media that should be incorporated in the plating
protocol is:
a. colistin nalidixic acid for Listeria
b. MacConkey agar with sorbitol for Campylobacter
c. mannitol salt agar for Enterococcus species
d. thiosulfate citrate bile salts sucrose for Vibrio species
Ans: thiosulfate citrate bile salts sucrose for Vibrio species
,Quiz 10. A male urethral discharge specimen submitted for culture should be
inoculated to:
a. sheep blood and phenylethyl alcohol agars
b. eosin-methylene blue and sheep blood agars
c. thioglycollate broth and chocolate agar
d. chocolate and modified Thayer-Martin agars
Ans: chocolate and modified Thayer-Martin agars
Quiz 11. The steam autoclave method of sterilization requires:
a. 15 Ib of pressure for 15 minutes
b. dry heat for 20 minutes
c. a maximum temperature of 100C
d. a source of ethylene oxide
Ans: 15 Ib of pressure for 15 minutes
Quiz 12. Which selective medium is used for the isolation of gram-positive
microorganisms?
a Columbia CNA with 5% sheep blood
b trypticase soy agar with 5% sheep blood
c eosin methylene blue
d modified Thayer-Martin
Ans: Columbia CNA with 5% sheep blood
Quiz 13. Campylobacter jejuni isolation requires the fecal specimen be:
a. inoculated onto selective plating media and incubated in reduced oxygen with
added CO2 at 42C
b. stored in tryptic soy broth before plating to ensure growth of the organism
c. inoculated onto selective plating media and incubated at both 35C and at room
temperature
d. incubated at 35C for 2 hours in Cary-Blair media before inoculating onto selective
plating media
Ans: inoculated onto selective plating media and incubated in reduced oxygen with
added CO2 at 42C
Quiz 14. Which of the following specimen requests is acceptable?
a. feces submitted for anaerobic culture
b. Foley catheter tip submitted for aerobic culture
c. rectal swab submitted for direct smear for gonococci
d. urine for culture of acid-fast bacilli
Ans: urine for culture of acid-fast bacilli
Quiz 15. A cerebrospinal fluid specimen containing only 2 drops CSF was collected
by a lumbar puncture from a febrile 25-year-old male and was submitted for a stat
Gram stain and culture. The direct specimen Gram stain was reported as many
, neutrophils and no microorganisms seen. The remaining drop of CSF should be
inoculated to:
a. blood agar
b. CNA agar
c. chocolate agar
d. Thayer-Martin agar
Ans: chocolate agar
Quiz 16. A diabetic foot swab from a 82-year-old woman with recurrent infections is
submitted for culture. The Gram stain reveals:
many neutrophils, no squamous epithelial cells
many gram-negative bacilli
many gram-positive cocci in chains
The physician requests that all pathogens be worked up. In addition to the sheep
blood, chocolate and MacConkey agar plates routinely used for wound cultures, the
technologist might also process a(n):
a anaerobic blood agar plate
b BCYE agar plate
c CNA agar plat
Ans: CNA agar plate
Quiz 17. Which of the following is the most appropriate specimen source and primary
media selection?
a. CSF Columbia CNA, MacConkey
b. endocervical chocolate, Martin Lewis
c. sputum sheep blood, Thayer-Martin, KV-laked blood
d. urine sheep blood, chocolate, Columbia CNA
Ans: endocervical chocolate, Martin Lewis
Quiz 18. Which of the following is the most appropriate organism and media
combination?
a. Vibrio species - Skirrow
b. Enterohemorrhagic E. coli - phenylethyl alcohol (PEA)
c. Campylobacter species - charcoal yeast extract
d. Yersinia enterocolitica - cefsulodin-irgasan-novobiocin (CIN)
Ans: Yersinia enterocolitica - cefsulodin-irgasan-novobiocin (CIN)
Quiz 19. A Gram stain from a swab of a hand would reveals: moderate neutrophils,
no squamous epithelial cells, moderate gram-positive cocci in clusters, moderate
large gram-negative bacilli. Select the appropriate media that will selectively isolate
each organism.
a. KV-laked agar, Thayer-Martin
b. sheep blood, MacConkey
c. Columbia CNA, chocolate
d. Columbia CNA, MacConkey
Revision Examination Tests
“Come all for this Greatness”
... 100% Correct Ans ...
BOC MICROBIOLOGY 6TH EDITION EXAM 1 2024-2025
QUESTIONS AND CORRECT DETAILED ANSWERS
1. The proper blood-to-broth ratio for blood cultures to reduce the antibacterial effect
of serum in adults is:
a. 1:2
b. 1:3
c. 1:10
d. 1:30
Ans: 1:10
Quiz 2. The most appropriate method for collecting a urine specimen from a patient
with an indwelling catheter is:
a. remove the catheter, cut the tip, and submit it for culture
b. disconnect the catheter from the bag, and aseptically collect urine from the
terminal end of the catheter
c. aseptically collect urine directly from the drainage bag
d. aspirate urine aseptically from the catheter tubing
Ans: aspirate urine aseptically from the catheter tubing
Quiz 3. Which of the following groups of specimens would be acceptable for
anaerobic culture?
a. vaginal swab, eye swab
b. intraoral surface swab, leg tissue
c. pleural fluid, brain abscess fluid
d. urine, sputum
Ans: pleural fluid, brain abscess fluid
Quiz 4. Sodium polyanetholsulfonate (SPS) is used as an anticoagulant for blood
cultures because it:
a. inactivates penicillin and cephalosporins
b. prevents clumping of red cells
c. inactivates neutrophils and components of serum complement
d. facilitates growth of anaerobes
,Ans: inactivates neutrophils and components of serum complement
Quiz 5. The optimal collection of a wound specimen for culture of anaerobic
organisms is a:
a. swab of lesion obtained before administration of antibiotics
b. swab of lesion obtained after administration of antibiotics
c. syringe filled with pus, obtained before administration of antibiotics
d. syringe filled with pus, obtained after administration of antibiotics
Ans: syringe filled with pus, obtained before administration of antibiotics
Quiz 6. The most important variable in the recovery of organisms in adult patients
with bacteremia (bacterial sepsis) is:
a. subculture of all bottles at day 5 of incubation
b. the recommended volume of blood cultured
c. collection of daily blood culture sets for 3 consecutive days
d. collection of multiple blood culture sets from a single venipuncture
Ans: the recommended volume of blood cultured
Quiz 7. Virus transport medium should contain agents that:
a. enable rapid viral growth during the transport time
b. inhibit bacterial and fungal growth
c. destroy nonpathogenic viruses
d. inhibit complement-fixing antibodies
Ans: inhibit bacterial and fungal growth
Quiz 8. A bronchoscopy sample with the request for culture of Legionella is sent to
the laboratory. The correct plating protocol is:
a. culture on thiosulfate citrate bile salt media
b. incubate the culture media anaerobically
c. reject the specimen and request a sputum sample
d. culture on buffered charcoal yeast extract agar with antibiotics
Ans: culture on buffered charcoal yeast extract agar with antibiotics
Quiz 9. A community hospital microbiology laboratory is processing significant
numbers of stool cultures because of an outbreak of diarrhea following heavy rains
and flooding in the county. A media that should be incorporated in the plating
protocol is:
a. colistin nalidixic acid for Listeria
b. MacConkey agar with sorbitol for Campylobacter
c. mannitol salt agar for Enterococcus species
d. thiosulfate citrate bile salts sucrose for Vibrio species
Ans: thiosulfate citrate bile salts sucrose for Vibrio species
,Quiz 10. A male urethral discharge specimen submitted for culture should be
inoculated to:
a. sheep blood and phenylethyl alcohol agars
b. eosin-methylene blue and sheep blood agars
c. thioglycollate broth and chocolate agar
d. chocolate and modified Thayer-Martin agars
Ans: chocolate and modified Thayer-Martin agars
Quiz 11. The steam autoclave method of sterilization requires:
a. 15 Ib of pressure for 15 minutes
b. dry heat for 20 minutes
c. a maximum temperature of 100C
d. a source of ethylene oxide
Ans: 15 Ib of pressure for 15 minutes
Quiz 12. Which selective medium is used for the isolation of gram-positive
microorganisms?
a Columbia CNA with 5% sheep blood
b trypticase soy agar with 5% sheep blood
c eosin methylene blue
d modified Thayer-Martin
Ans: Columbia CNA with 5% sheep blood
Quiz 13. Campylobacter jejuni isolation requires the fecal specimen be:
a. inoculated onto selective plating media and incubated in reduced oxygen with
added CO2 at 42C
b. stored in tryptic soy broth before plating to ensure growth of the organism
c. inoculated onto selective plating media and incubated at both 35C and at room
temperature
d. incubated at 35C for 2 hours in Cary-Blair media before inoculating onto selective
plating media
Ans: inoculated onto selective plating media and incubated in reduced oxygen with
added CO2 at 42C
Quiz 14. Which of the following specimen requests is acceptable?
a. feces submitted for anaerobic culture
b. Foley catheter tip submitted for aerobic culture
c. rectal swab submitted for direct smear for gonococci
d. urine for culture of acid-fast bacilli
Ans: urine for culture of acid-fast bacilli
Quiz 15. A cerebrospinal fluid specimen containing only 2 drops CSF was collected
by a lumbar puncture from a febrile 25-year-old male and was submitted for a stat
Gram stain and culture. The direct specimen Gram stain was reported as many
, neutrophils and no microorganisms seen. The remaining drop of CSF should be
inoculated to:
a. blood agar
b. CNA agar
c. chocolate agar
d. Thayer-Martin agar
Ans: chocolate agar
Quiz 16. A diabetic foot swab from a 82-year-old woman with recurrent infections is
submitted for culture. The Gram stain reveals:
many neutrophils, no squamous epithelial cells
many gram-negative bacilli
many gram-positive cocci in chains
The physician requests that all pathogens be worked up. In addition to the sheep
blood, chocolate and MacConkey agar plates routinely used for wound cultures, the
technologist might also process a(n):
a anaerobic blood agar plate
b BCYE agar plate
c CNA agar plat
Ans: CNA agar plate
Quiz 17. Which of the following is the most appropriate specimen source and primary
media selection?
a. CSF Columbia CNA, MacConkey
b. endocervical chocolate, Martin Lewis
c. sputum sheep blood, Thayer-Martin, KV-laked blood
d. urine sheep blood, chocolate, Columbia CNA
Ans: endocervical chocolate, Martin Lewis
Quiz 18. Which of the following is the most appropriate organism and media
combination?
a. Vibrio species - Skirrow
b. Enterohemorrhagic E. coli - phenylethyl alcohol (PEA)
c. Campylobacter species - charcoal yeast extract
d. Yersinia enterocolitica - cefsulodin-irgasan-novobiocin (CIN)
Ans: Yersinia enterocolitica - cefsulodin-irgasan-novobiocin (CIN)
Quiz 19. A Gram stain from a swab of a hand would reveals: moderate neutrophils,
no squamous epithelial cells, moderate gram-positive cocci in clusters, moderate
large gram-negative bacilli. Select the appropriate media that will selectively isolate
each organism.
a. KV-laked agar, Thayer-Martin
b. sheep blood, MacConkey
c. Columbia CNA, chocolate
d. Columbia CNA, MacConkey
