Microbiology Lab Exam #1 Study
Guide Questions and Answers 100%
Pass
The total magnification of the oil immersion objective is: - ✔✔1000
Ocular/Eyepiece - ✔✔Binocular microscopes have two. They magnify the image
tenfold.
Body - ✔✔It contains mirrors that bend the light into the ocular.
Nosepiece - ✔✔Where the objectives are attached to.
Objectives - ✔✔Contains lenses that magnify the image. A microscope has 4x, 10x, 40x
and 100x
Arm - ✔✔It supports the nosepiece, holds on/off toggle and focus adjustment knobs.
Stage/Mechanical stage - ✔✔The black, flat platform on which your slide rests. Its the
silver apparatus that actually holds the slide and moves the slide to position it on the
stage.
Stage Adjustment knobs - ✔✔These move the the mechanical stage
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, Light Intensity Control - ✔✔This knob increases or decreases light output from the light
source.
Substage condenser - ✔✔It is made of at least two lenses that focus the light passing
through the specimen and improve image sharpness.
Aperture diaphragm control - ✔✔Controls the amount of light entering the substage
condenser from the light source.
Field diaphragm lever - ✔✔It opens and closes to increase and decrease light from the
light source. It should remain open
Light source - ✔✔The illuminator is a halogen bulb that produces light.
Base - ✔✔It holds the illuminator and supports the rest of the microscope.
Fine focus adjustment knob - ✔✔Brings the image into sharp focus. This knob raises
and lowers the stage in very small increments.
Coarse focus adjustment knob - ✔✔Moves the stage up and down by larger increments
and allows you to bring the image into focus.
Resolution - ✔✔The ability of a lens system to distinguish as separate entities objects
that are very small and very close together. It is determined by the quality of the lens
and the wavelength of light used.
Magnification - ✔✔Make a small object appear larger.
Katelyn Whitman, All Rights Reserved © 2025 2
Guide Questions and Answers 100%
Pass
The total magnification of the oil immersion objective is: - ✔✔1000
Ocular/Eyepiece - ✔✔Binocular microscopes have two. They magnify the image
tenfold.
Body - ✔✔It contains mirrors that bend the light into the ocular.
Nosepiece - ✔✔Where the objectives are attached to.
Objectives - ✔✔Contains lenses that magnify the image. A microscope has 4x, 10x, 40x
and 100x
Arm - ✔✔It supports the nosepiece, holds on/off toggle and focus adjustment knobs.
Stage/Mechanical stage - ✔✔The black, flat platform on which your slide rests. Its the
silver apparatus that actually holds the slide and moves the slide to position it on the
stage.
Stage Adjustment knobs - ✔✔These move the the mechanical stage
Katelyn Whitman, All Rights Reserved © 2025 1
, Light Intensity Control - ✔✔This knob increases or decreases light output from the light
source.
Substage condenser - ✔✔It is made of at least two lenses that focus the light passing
through the specimen and improve image sharpness.
Aperture diaphragm control - ✔✔Controls the amount of light entering the substage
condenser from the light source.
Field diaphragm lever - ✔✔It opens and closes to increase and decrease light from the
light source. It should remain open
Light source - ✔✔The illuminator is a halogen bulb that produces light.
Base - ✔✔It holds the illuminator and supports the rest of the microscope.
Fine focus adjustment knob - ✔✔Brings the image into sharp focus. This knob raises
and lowers the stage in very small increments.
Coarse focus adjustment knob - ✔✔Moves the stage up and down by larger increments
and allows you to bring the image into focus.
Resolution - ✔✔The ability of a lens system to distinguish as separate entities objects
that are very small and very close together. It is determined by the quality of the lens
and the wavelength of light used.
Magnification - ✔✔Make a small object appear larger.
Katelyn Whitman, All Rights Reserved © 2025 2
