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  • Biol 200
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  • Biol 200

biol 200 Test - questions and Answers all Verified 100% Correct

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  • January 10, 2025
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  • Exam (elaborations)
  • Questions & answers
  • Biol 200
  • Biol 200
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biol 200 Test - questions and Answers all
Verified 100% Correct

what are two important properties of ligand-binding - ✔✔specificity: the ability of a
protein to bind only one particular ligand even in the presence of a vast excess of
irrelevant molecules.
affinity: the tightness or strength of binding expressed as dissociation constant (kd). the
stronger the interaction the lower the kd.



what is binding? - ✔✔an interaction bewtween comementary molecular surfaces



what kind of reactions do protein binding specificity arise from? - ✔✔interaction
which are individually weak but collectively strong. COMPLIMENTARY



What is the main function of antibodies? - ✔✔bind to antigens with high specificty
and high affinity


What is the structure of antibodies in terms of heavy and light chains? -
✔✔anitbodies contain two identical heavy chains and two identical light chains.



Which part of the antibody binds to the antigen? - ✔✔he antigen bidning surface or
CDR (complementarity determining region) involves multiple protein loops from both
the heavy and the light chains


What is the significance of the Complementarity Determining Region (CDR) in
antibodies? How is the variation in the CDR regions of antibodies described?
Why is the repertoire of possible CDRs in the antibody population considered
astronomical? - ✔✔these loops are highly variable in amino acid sequence among

, antibody encoding genes so the total repretoire of possible CDRs in the antibody
population is astronomical


where on the enzyme does substrate specificity arise from - ✔✔substrate specifcity
arises from the substrate binding site



two sites on the active site - ✔✔The two main sites at the enzyme's active site are
the substrate binding site, where the substrate binds, and the catalytic site, where the
chemical reaction is facilitated.



what occurs at the active site - ✔✔substrate binding and reaction catalysis occur at
the enzymes active site.



what determine the properties of the binding site on enzymes - ✔✔a.a side chains
around the catalytic site. some amino acids in the enzyme polypeptide contribute to the
substrate binding site and others contribute to the catalytic site.



vmax? what does it depend on - ✔✔the max rate of catalysis given saturating
amounts of substrate. depends on the amount of enzyme and how fast it can work



km? - ✔✔km: the substrate concentration that supports a rate of catalysis equal to
one half of the vmax


What happens when you increase the concentration of enzyme in a reaction, and how
does it affect the Vmax and Km? - ✔✔4 times as much enzyme in the reaction gives a
vmax 4-fold higherbut the measured km is the same because in general the binding
affinity is independent of concetrations but depends only on the chemical porperties of
the enzyme and the substrate.

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