Biochemistry
Applied Molecular Biology
Compiled By Simon Mwangi
Edition: 2024/25
, Science | Biochemistry I of IV pages
1. Mutations resulting in a change of restriction sites in a gene can be used to perform pedigree analysis of
In other words, the same gene of two individuals may be cleaved differently by a given restriction enzyme and
Restriction Fragment Length Polymorphism (RFLP)
2. If RFLP occurs as a result of mutation in DNA coding for an essential protein, the RFLP can be used to do
diagnose the disease; identify the individual (forensic pathology)
3. What is the molecular basis for Sickle Cell Anemia?
substitution of T and A in the template strand of the sixth codon for beta-globulin changes the codon from
4. What physical properties of hemoglobin are effected in SCA?
at reduced oxygen tension, the hemoglobin precipitates
5. What is the basis of detection for SCA?
loss of restriction enzyme site (MstII) - when this gene is amplified (PCR) and subjected to treatment with
6. If the mutation is known, one can rapidly determine genotype of patient by hybridization of ____ ____
alley specific oligonucleotides (ASOs)
7. The basis of DNA finger printing is RFLP. Because some mutations some segments of DNAs from different
different sized fragments when digested with enzymes
8. Polymorphism in angiotensinogen is being used to predict ____ in cardiac patients
hypertension
9. Certain sections of DNA are known to be hypervariable. What does this mean?
no two individuals (excluding identical twins) possess the exact same restriction sites in this region - can be
10. Gene chips or ____ are revolutionary new technology for analyzing ____ _____
microarrays; gene expression
11. In regards to gene chips, hundreds of unique gene fragments (DNA) are fixed to glass slides by robotic
stations used in computer chip assembly. RNA isolated form cells or tissues can be converted to ____ with
cDNA
note: synthesis occurs with incorporation of fluorescently labeled nucleotides
12. In regards to gene chips, _____ of fluorescently labeled cDNA to DNA spots on the chip - each of which
hybridization
13. In regards to gene chips, if two different cell populations are carried through the procedure and each
pool is labeled with a different florescent nucleotide, then a differential expression level will result as the
true
14. What are the major problems of gene therapy?
efficiency of integration and expression of therapeutic genes
15. In regards to gene therapy, in many cases only about 5-10% of normal expression may be enough to bring
Biochemistry 2024/25 Edition
Applied Molecular Biology
Compiled By Simon Mwangi
Edition: 2024/25
, Science | Biochemistry I of IV pages
1. Mutations resulting in a change of restriction sites in a gene can be used to perform pedigree analysis of
In other words, the same gene of two individuals may be cleaved differently by a given restriction enzyme and
Restriction Fragment Length Polymorphism (RFLP)
2. If RFLP occurs as a result of mutation in DNA coding for an essential protein, the RFLP can be used to do
diagnose the disease; identify the individual (forensic pathology)
3. What is the molecular basis for Sickle Cell Anemia?
substitution of T and A in the template strand of the sixth codon for beta-globulin changes the codon from
4. What physical properties of hemoglobin are effected in SCA?
at reduced oxygen tension, the hemoglobin precipitates
5. What is the basis of detection for SCA?
loss of restriction enzyme site (MstII) - when this gene is amplified (PCR) and subjected to treatment with
6. If the mutation is known, one can rapidly determine genotype of patient by hybridization of ____ ____
alley specific oligonucleotides (ASOs)
7. The basis of DNA finger printing is RFLP. Because some mutations some segments of DNAs from different
different sized fragments when digested with enzymes
8. Polymorphism in angiotensinogen is being used to predict ____ in cardiac patients
hypertension
9. Certain sections of DNA are known to be hypervariable. What does this mean?
no two individuals (excluding identical twins) possess the exact same restriction sites in this region - can be
10. Gene chips or ____ are revolutionary new technology for analyzing ____ _____
microarrays; gene expression
11. In regards to gene chips, hundreds of unique gene fragments (DNA) are fixed to glass slides by robotic
stations used in computer chip assembly. RNA isolated form cells or tissues can be converted to ____ with
cDNA
note: synthesis occurs with incorporation of fluorescently labeled nucleotides
12. In regards to gene chips, _____ of fluorescently labeled cDNA to DNA spots on the chip - each of which
hybridization
13. In regards to gene chips, if two different cell populations are carried through the procedure and each
pool is labeled with a different florescent nucleotide, then a differential expression level will result as the
true
14. What are the major problems of gene therapy?
efficiency of integration and expression of therapeutic genes
15. In regards to gene therapy, in many cases only about 5-10% of normal expression may be enough to bring
Biochemistry 2024/25 Edition
