HESI MICROBIOLOGY TEST BANK 2024-2025
UPDATE MIDTERM EXAM ALL 58
QUESTIONS WITH CORRECT ANSWERS
ALREADY GRADED A+|REAL EXAM!!
Place the steps of the gram stain in the correct order: 1-alcohol-acetone; 2-crystal violet 3-safranin; 4-
iodine. - ANSWERS -2, 4, 1, 3
Which of the following organelles most closely resembles a prokaryotic cell? - ANSWERS -
mitochondrion
Which of the following is NOT a product of transcription? - ANSWERS -a new strand of DNA
E. coli makes insulin because - ANSWERS -the insulin gene was inserted into it
This microscope produces an image of a light cell against a dark background; internal structures are NOT
visible. - ANSWERS -darkfield microscope
The appearance of gram-negative bacteria after addition of the mordant in the Gram stain. - ANSWERS -
Colorless
PCR can be used to amplify DNA in a clinical sample. The following steps are used in PCR. What is the
fourth step? - ANSWERS -Incubate at 60 degrees Celsius
The appearance of gram-positive bacteria after addition of the first dye in the Gram stain - ANSWERS -
purple
Who proved that microorganisms cause disease? - ANSWERS -Pasteur
, In Figure 6.1 ,which line best depicts an obligate anaerobe in the presence of O2? - ANSWERS -Line C
Which microscope is used to observe a specimen that emits light when illuminated with an ultraviolet
light? - ANSWERS -fluorescence microscope
A gene is best defined as - ANSWERS -a sequence of nucleotides in DNA that codes for a functional
product
You want to determine whether a person has a certain mutant gene. The process involves using a primer
and TAQ. This process is - ANSWERS -PCR
Which of the following structures is NOT found in prokaryotic cells? - ANSWERS -Cillium
Which of the following does NOT achieve sterilization? - ANSWERS -Pasteurization
A strictly fermentative bacterium produces energy - ANSWERS -Only in the presence of oxygen
In figure 6.1, which line best depicts a mesophile with an optimum temperature of 35C incubated at 40C
- ANSWERS -B
Regarding Pasteur's experiments with the S-neck flask, which of the following statements is true? -
ANSWERS -all of the above
Which organism is NOT correctly matched to its energy source? - ANSWERS -photoautotroph - CO2
In figure 6.1, which line shoes the growth of an obligate aero be incubated anaerobically? - ANSWERS -C
Table 8.2
UPDATE MIDTERM EXAM ALL 58
QUESTIONS WITH CORRECT ANSWERS
ALREADY GRADED A+|REAL EXAM!!
Place the steps of the gram stain in the correct order: 1-alcohol-acetone; 2-crystal violet 3-safranin; 4-
iodine. - ANSWERS -2, 4, 1, 3
Which of the following organelles most closely resembles a prokaryotic cell? - ANSWERS -
mitochondrion
Which of the following is NOT a product of transcription? - ANSWERS -a new strand of DNA
E. coli makes insulin because - ANSWERS -the insulin gene was inserted into it
This microscope produces an image of a light cell against a dark background; internal structures are NOT
visible. - ANSWERS -darkfield microscope
The appearance of gram-negative bacteria after addition of the mordant in the Gram stain. - ANSWERS -
Colorless
PCR can be used to amplify DNA in a clinical sample. The following steps are used in PCR. What is the
fourth step? - ANSWERS -Incubate at 60 degrees Celsius
The appearance of gram-positive bacteria after addition of the first dye in the Gram stain - ANSWERS -
purple
Who proved that microorganisms cause disease? - ANSWERS -Pasteur
, In Figure 6.1 ,which line best depicts an obligate anaerobe in the presence of O2? - ANSWERS -Line C
Which microscope is used to observe a specimen that emits light when illuminated with an ultraviolet
light? - ANSWERS -fluorescence microscope
A gene is best defined as - ANSWERS -a sequence of nucleotides in DNA that codes for a functional
product
You want to determine whether a person has a certain mutant gene. The process involves using a primer
and TAQ. This process is - ANSWERS -PCR
Which of the following structures is NOT found in prokaryotic cells? - ANSWERS -Cillium
Which of the following does NOT achieve sterilization? - ANSWERS -Pasteurization
A strictly fermentative bacterium produces energy - ANSWERS -Only in the presence of oxygen
In figure 6.1, which line best depicts a mesophile with an optimum temperature of 35C incubated at 40C
- ANSWERS -B
Regarding Pasteur's experiments with the S-neck flask, which of the following statements is true? -
ANSWERS -all of the above
Which organism is NOT correctly matched to its energy source? - ANSWERS -photoautotroph - CO2
In figure 6.1, which line shoes the growth of an obligate aero be incubated anaerobically? - ANSWERS -C
Table 8.2
