ASCP MLS CERTIFICATION EXAM
LATEST ACTUAL EXAM 2025
STUDYGUIDE QUESTIONS AND
DETAILED CORRECT ANSWERS | A+
GRADE GUARANTEED PASS
Estrogen and progesterone markers are most commonly
used to provide prognostic information about: Correct
Answer Breast cancer
The prozone effect ( when performing a screening titer) is
most likely to result in: Correct Answer Prozone effect
(due to antibody excess) will result in an initial false
negative in spite of the large amount of antibody in the
serum, followed by a positive result as the specimen is
diluted.
Which of the following Rh antigens is found the highest
frequency in the Caucasian population:
A. C
B. E
C. c
D. e Correct Answer D. e
The accuracy of an immunoassay is its ability to
discriminate between results that are true positives and
results that are true negatives. Two parameters of test
accuracy are specificity and sensitivity. Which of these
,statements apply to an immunoassay with high specificity,
but low sensitivity? Correct Answer Accurately identifies
the absence of disease and has few false-positives
A test with high specificity accurately detects the absence
of disease. The more specific a test is, the fewer false-
positive results will occur. A test with high sensitivity
accurately identifies the presence of disease. The more
sensitive a test, the fewer false-negative results it
produces. In the case stated in this question, the
immunoassay has high specificity, so it has few false-
positives and will accurately detect those individuals who
do not have the disease or condition that is being tested
for. However, the test has low sensitivity, so it may not
identify all individuals who actually have the disease; it
may produce many false-negative results.
Distinguishing characteristics of Micrococcus luteus:
Correct Answer Resistance to furazolidone is one of the
key characteristics by which members of the genus
Micrococcus (resistant) can be separated from members
of the genus Staphylococcus (susceptible). Micrococcus
species also are susceptible to novobiocin and to
bacitracin, although the zone of inhibition to the "A" disk is
often small (as shown in this photograph).
The sickling phenomenon observed in erythrocytes
containing hemoglobin S may be induced by which of the
following? Correct Answer Reducing agents actually
induce the sickling phenomenon in RBCs when they
contain hemoglobin S.
,If an autologous blood donor weighs 35 kg, how much
blood can be collected for later transfusion? Correct
Answer 315 mL
Volume to draw = (Donor's weight in kilograms / 50 kg
(minimum weight requirement) x 450 mL (volume of unit to
be donated)
In this case, Volume to draw = (35 kg / 50 kg) x 450 mL =
315 mL
Which adipokine is decreased in obesity, is anti-
inflammatory, and increases insulin sensitivity? Correct
Answer Adiponectin is a protective cytokine. This cytokine
is anti-inflammatory and increases insulin sensitivity.
Adiponectin is usually decreased in obesity.
Distinguishing characteristics of Mycobacterium fortuitum:
Correct Answer Mycobacterium fortuitum is positive for
iron uptake, nitrate, arysulfatase, and growth on
MacConkey agar without crystal violet in five days.
CD5 antigen is normally found on which of the following
lymphocyte populations? Correct Answer Mature T cells
Define: Congestive heart failure Correct Answer A left
ventricular dysfunction resulting from aging, hypertension,
atherosclerosis or muscle damage from an AMI or
repeated AMIs.
, Define infarction: Correct Answer An area of tissue death
that occurs due to lack of oxygen.
Define: Ischemia Correct Answer An inadequate blood
supply that decreases availability of oxygen.
Define: Angina Correct Answer Chest pain caused by
inadequate supply of oxygen to heart myocardium.
Which of the following antigen groups is closely related to
the ABO antigens on the red cell membrane? Correct
Answer I and i antigens are related biochemically and
spatially to the ABH and Lewis antigens on the red cell
membrane. Anti-I is usually an autoantibody. It is common
as a benign cold autoagglutinin. Strong anti-I is associated
with Mycoplasma pneumonia infection, where it may be
associated with cold autoimmune hemolytic anemia. Anti-I
is characterized by its failure to react with cord red cells.
Anti-i generally occurs as a cold reactive IgM antibody in
the setting of infectious mononucleosis.
Distinguishing between T lymphs and B lymphs: Correct
Answer T lymphocytes are larger and have more vacuoles
than B lymphocytes.
Define Galactosemia: Correct Answer Galactosemia is an
inherited metabolic disorder that affects an individual's
ability to metabolize the sugar galactose effectively.
Infants affected by galactosemia typically present with
symptoms of lethargy, vomiting, diarrhea, failure to thrive,
and jaundice. The presence of galactose in the urine
LATEST ACTUAL EXAM 2025
STUDYGUIDE QUESTIONS AND
DETAILED CORRECT ANSWERS | A+
GRADE GUARANTEED PASS
Estrogen and progesterone markers are most commonly
used to provide prognostic information about: Correct
Answer Breast cancer
The prozone effect ( when performing a screening titer) is
most likely to result in: Correct Answer Prozone effect
(due to antibody excess) will result in an initial false
negative in spite of the large amount of antibody in the
serum, followed by a positive result as the specimen is
diluted.
Which of the following Rh antigens is found the highest
frequency in the Caucasian population:
A. C
B. E
C. c
D. e Correct Answer D. e
The accuracy of an immunoassay is its ability to
discriminate between results that are true positives and
results that are true negatives. Two parameters of test
accuracy are specificity and sensitivity. Which of these
,statements apply to an immunoassay with high specificity,
but low sensitivity? Correct Answer Accurately identifies
the absence of disease and has few false-positives
A test with high specificity accurately detects the absence
of disease. The more specific a test is, the fewer false-
positive results will occur. A test with high sensitivity
accurately identifies the presence of disease. The more
sensitive a test, the fewer false-negative results it
produces. In the case stated in this question, the
immunoassay has high specificity, so it has few false-
positives and will accurately detect those individuals who
do not have the disease or condition that is being tested
for. However, the test has low sensitivity, so it may not
identify all individuals who actually have the disease; it
may produce many false-negative results.
Distinguishing characteristics of Micrococcus luteus:
Correct Answer Resistance to furazolidone is one of the
key characteristics by which members of the genus
Micrococcus (resistant) can be separated from members
of the genus Staphylococcus (susceptible). Micrococcus
species also are susceptible to novobiocin and to
bacitracin, although the zone of inhibition to the "A" disk is
often small (as shown in this photograph).
The sickling phenomenon observed in erythrocytes
containing hemoglobin S may be induced by which of the
following? Correct Answer Reducing agents actually
induce the sickling phenomenon in RBCs when they
contain hemoglobin S.
,If an autologous blood donor weighs 35 kg, how much
blood can be collected for later transfusion? Correct
Answer 315 mL
Volume to draw = (Donor's weight in kilograms / 50 kg
(minimum weight requirement) x 450 mL (volume of unit to
be donated)
In this case, Volume to draw = (35 kg / 50 kg) x 450 mL =
315 mL
Which adipokine is decreased in obesity, is anti-
inflammatory, and increases insulin sensitivity? Correct
Answer Adiponectin is a protective cytokine. This cytokine
is anti-inflammatory and increases insulin sensitivity.
Adiponectin is usually decreased in obesity.
Distinguishing characteristics of Mycobacterium fortuitum:
Correct Answer Mycobacterium fortuitum is positive for
iron uptake, nitrate, arysulfatase, and growth on
MacConkey agar without crystal violet in five days.
CD5 antigen is normally found on which of the following
lymphocyte populations? Correct Answer Mature T cells
Define: Congestive heart failure Correct Answer A left
ventricular dysfunction resulting from aging, hypertension,
atherosclerosis or muscle damage from an AMI or
repeated AMIs.
, Define infarction: Correct Answer An area of tissue death
that occurs due to lack of oxygen.
Define: Ischemia Correct Answer An inadequate blood
supply that decreases availability of oxygen.
Define: Angina Correct Answer Chest pain caused by
inadequate supply of oxygen to heart myocardium.
Which of the following antigen groups is closely related to
the ABO antigens on the red cell membrane? Correct
Answer I and i antigens are related biochemically and
spatially to the ABH and Lewis antigens on the red cell
membrane. Anti-I is usually an autoantibody. It is common
as a benign cold autoagglutinin. Strong anti-I is associated
with Mycoplasma pneumonia infection, where it may be
associated with cold autoimmune hemolytic anemia. Anti-I
is characterized by its failure to react with cord red cells.
Anti-i generally occurs as a cold reactive IgM antibody in
the setting of infectious mononucleosis.
Distinguishing between T lymphs and B lymphs: Correct
Answer T lymphocytes are larger and have more vacuoles
than B lymphocytes.
Define Galactosemia: Correct Answer Galactosemia is an
inherited metabolic disorder that affects an individual's
ability to metabolize the sugar galactose effectively.
Infants affected by galactosemia typically present with
symptoms of lethargy, vomiting, diarrhea, failure to thrive,
and jaundice. The presence of galactose in the urine
