1|Page | © copyright 2024/2025 | Grade A+
NWHSU Biochem 1 - Exam 2
(Enzymes and Carbohydrates)
Questions and Answers (100% Pass)
Enzymes
✓ -Increase reaction rate (Vo) - "velocity"; M/min or mol/min
✓ -Are not used up in reaction
Enzyme functions under mild conditions
✓ - temperature
✓ - pH
✓ - pressure
Structure of Enzymes
✓ - vast majority are proteins
✓ - many need a non-amino acid component to be functional
Function of Enzymes
✓ Enzymes increase velocity by which product is formed over time
Enzyme kinetics
Master01 | October, 2024/2025 | Latest update
, 1|Page | © copyright 2024/2025 | Grade A+
✓ - Helps understand and predict enzymatic reactions and factors
affecting them
✓ - Studying reaction rate vs [S] relationships
ADH
✓ Alcohol Dehydrogenase
Reaction catalyzed by ADH
✓ Ethanol (substrate) - (remove 2 C-) -> Acetaldehyde (Product)
Hyperbola
✓ What is the ADH (Vo vs [S]) plot form?
at the active site instead of the substrate
✓ A competitive inhibitor binds...
reversible non-covalent
✓ Feedback inhibition is an example of ______ inhibition
vitamin K dependent
Master01 | October, 2024/2025 | Latest update
, 1|Page | © copyright 2024/2025 | Grade A+
✓ Glutamate carboxylase involved in blood clotting is _____
Noncovalent, reversible, enzyme activation
✓ allosteric
Proteases in the small intestine get activated by...
✓ proteolytic cleavage of C- and/or N- terminus
3 types of reversible inhibition
✓ competitive
✓ uncompetitive
✓ mixed
Competitive Inhibition
✓ Inhibitor has to have similar structure to Substrate (e.g. methanol
(CH3OH) is seminal to ethanol (CH3CH2OH)
When the lines (with or without) intersect on y-axis on the LB graph, which
inhibition is it?
✓ competitive
Master01 | October, 2024/2025 | Latest update
NWHSU Biochem 1 - Exam 2
(Enzymes and Carbohydrates)
Questions and Answers (100% Pass)
Enzymes
✓ -Increase reaction rate (Vo) - "velocity"; M/min or mol/min
✓ -Are not used up in reaction
Enzyme functions under mild conditions
✓ - temperature
✓ - pH
✓ - pressure
Structure of Enzymes
✓ - vast majority are proteins
✓ - many need a non-amino acid component to be functional
Function of Enzymes
✓ Enzymes increase velocity by which product is formed over time
Enzyme kinetics
Master01 | October, 2024/2025 | Latest update
, 1|Page | © copyright 2024/2025 | Grade A+
✓ - Helps understand and predict enzymatic reactions and factors
affecting them
✓ - Studying reaction rate vs [S] relationships
ADH
✓ Alcohol Dehydrogenase
Reaction catalyzed by ADH
✓ Ethanol (substrate) - (remove 2 C-) -> Acetaldehyde (Product)
Hyperbola
✓ What is the ADH (Vo vs [S]) plot form?
at the active site instead of the substrate
✓ A competitive inhibitor binds...
reversible non-covalent
✓ Feedback inhibition is an example of ______ inhibition
vitamin K dependent
Master01 | October, 2024/2025 | Latest update
, 1|Page | © copyright 2024/2025 | Grade A+
✓ Glutamate carboxylase involved in blood clotting is _____
Noncovalent, reversible, enzyme activation
✓ allosteric
Proteases in the small intestine get activated by...
✓ proteolytic cleavage of C- and/or N- terminus
3 types of reversible inhibition
✓ competitive
✓ uncompetitive
✓ mixed
Competitive Inhibition
✓ Inhibitor has to have similar structure to Substrate (e.g. methanol
(CH3OH) is seminal to ethanol (CH3CH2OH)
When the lines (with or without) intersect on y-axis on the LB graph, which
inhibition is it?
✓ competitive
Master01 | October, 2024/2025 | Latest update
