UConn MCB 2610 Final Lab Practical (Questions & Answers) Rated 100%

Chemicals used to decrease the number of microbes on living tissues are called _____ - Antiseptics A Durham tube is included in the LT broth tubes to detect _____, a byproduct of lactose fermentation. - Gas _____ (lytic/lysogenic) phages can integrate its DNA into the host genome. - Lytic _____ will occur around the colonies, indicating amylase production and starch utilization. - Red dying from iodine _______ is an opportunistic pathogen that commonly causes staph infections. - Staphylococcus aureus A _____ agent does not kill a microorganism, but rather just inhibits its growth. - Bacteriostatic A clear area due to lysis by phage in a lawn of host cells is called a - Plaque A confluent growth of cells in the soft agar is called a _____ - Lawn An antibiotic that is effective against a wide varity of pathogenic microorganisms is called a _____ antibiotic. - Broad spectrum Antiseptic chemicals are used on _____. - Living tissue Bacteria can prevent phage attachment and escape infection by acquiring mutations in genes which alter their _____ - Cell surface receptorsChemicals used to decrease the number of microbes on inanimate objects are called _____ - Disinfectants Coliforms are a subgroup of enteric bacteria classified by their ability to ferment which sugar? - Lactose Define generation time. - Time for population to double During the lytic infection process, what does the phage particle inject into the host cells? - DNA/genome Given an example of a fomite. - Anything that people touch/use regularly Growth concentrated at the bottom of a thioglycolate broth tube indicates the organism is an aerobe/anaerobe? - Anaerobe Growth only along the path of the inoculating needle indicates that the bacteria is motile/non-motile. - Non-motile How is casein hydrolysis detected? - Clearing around growth How many times do you get culture from the tube while performing a t-streak? - Once If a nitrate broth tube does not change color after adding nitrate reagents A&B and zinc, did nitrate reduction occur? - Yes If a nitrate broth tube does not change color after the addition of nitrate reagents A&B, but turns red with the addition of zinc, did nitrate reduction occur? - NoIf a nitrate broth tube turns red after addition of nitrate reagents A&B, did nitrate reduction occur? - Yes In a LT broth tube, in order to detect the fermentation of lactose, a ____ is used to capture gas production. - Durham tube In a plaque assay, ______ and ______ are mixed together with soft agar, poured onto a plate, and incubated.