Basic Laboratory Principles and
Techniques 9th Edition by Bishop Fody
Questions & Answers with rationales
CHEMICALS - ANS■is a substance that occurs naturally or is obtained through a chemical
process that is used to produce a chemical effect or reaction.
American Chemical Society (ACS) - ANSestablished the specifications for grading of chemicals
1.Analytical Reagent (AR) Grade
2.Ultrapure Grade
3.Chemically Pure (CP) Grade
4.USP and NF Grade
5.Technical or Commercial Grade - ANSFive Grades of Chemicals (based on purity or quality):
ANALYTICAL REAGENT GRADE - ANS■are of very high purity.
■are used in the preparation of reagent in the clinical laboratory.
ULTRAPURE GRADE CAIM - ANS■Have been put through several purification steps.
■Extremely pure chemicals.
■Used for chromatography, atomic absorption, immunoassays, molecular diagnostics and etc.
■Carry designations of HPLC or chromatography.
CHEMICALLY PURE GRADE - ANS■do not state the impurity limitations and the preparations
of those chemicals are not uniform.
■Given that parameter, they are not recommended for reagent preparations in the clinical
laboratory.
■Commonly used in laboratory experiments in schools.
USP AND NF PURE GRADE - ANS■chemicals are not injurious to humans and are used to
produce drugs.
■Although they are adequate for human consumption, they maybe pure enough for specific
chemical application.
■The purity standards are not based on the needs of the laboratory.
USP - ANSUnited States Pharmacopoeia