BIO250L Lab 2 Culturing & Aseptic Technique SPRING 2024 Straighterline

Lab Report Format Expectations Utilize college level grammar and professional formatting when completing this worksheet. Submissions without proper formatting, all required photos or sufficient responses will be rejected. Pre-lab Questions 1. This lab includes two experiments that each look at different aspects of culturing microorganisms onto growth media. What concepts does each experiment focus on? Why do you think these concepts are important to the field of microbiology? The first experiment is about preparing agar plates and putting bacteria on them. It talks about why it's important to be really careful to keep things clean and make sure the bacteria grow without anything else mixing in. This is super important in microbiology so we can accurately figure out what types of bacteria we're dealing with without any extra stuff getting in the way. The second experiment is about moving bacteria to stab tubes and agar plates. It shows how bacteria grow differently in various conditions. This helps us learn how to grow and look at bacteria in different types of media. These ideas are also really crucial in microbiology for figuring out infections and studying how bacteria act. 2. In this lab, the experiments will allow or call for the use of four inoculation tools. List these tools and provide a scenario in which you would use each tool. ○ The loop is utilized for streaking microorganisms on a plate, whereas the needle is employed for picking and transferring colonies onto agar plates. ○ Pipettes are used to take liquid samples precisely. ○ Microbial colonies from the body's tissues are picked using swabs.. ○ he spreader evenly distributes bacterial colonies on a plate. 3. Describe a piece of equipment used in biotech labs to extend the growth pattern of microbes. Focus particularly on those used with E. coli used to make human insulin. At Biotech, bioreactors are utilized to create the perfect environment for E. coli to grow. 4. You’re a physician trying to isolate bacterial colonies from the human gut in an attempt to diagnose a gastrointestinal infection. You streak your sample on a growth media containing glucose, amino acids, and salts containing both sulfur and phosphorus with a pH of 7. You incubate the plates in aerobic conditions at 37 ˚C for three days, at which point you can see