AQA GCSE COMBINED SCIENCE: SYNERGY 8465/2H Higher Tier Paper 2 Life and Environmental Sciences Version: 1.0 Final G/KL/Jun23/E6 8465/2H (JUN2384652H01) GCSE COMBINED SCIENCE: SYNERGY Higher Tier Paper 2 Life and Environmental SciencesQUESTION PAPER & MARK

GCSE COMBINED SCIENCE: SYNERGY 8465/2H Higher Tier Paper 2 Life and Environmental Sciences Version: 1.0 Final G/KL/Jun23/E6 8465/2H (JUN2384652H01) GCSE COMBINED SCIENCE: SYNERGY Higher Tier Paper 2 Life and Environmental Sciences Thursday 25 May 2023 Morning Time allowed: 1 hour 45 minutes Materials For this paper you must have:  a ruler  a protractor  a scientific calculator  the periodic table (enclosed)  the Physics Equations Sheet (enclosed). Instructions  Use black ink or black ball-point pen. Pencil should only be used for drawing.  Fill in the boxes at the top of this page.  Answer all questions in the spaces provided. Do not write outside the box around each page or on blank pages.  If you need extra space for your answer(s), use the lined pages at the end of this book. Write the question number against your answer(s).  Do all rough work in this book. Cross through any work you do not want to be marked.  In all calculations, show clearly how you work out your answer. Information  The maximum mark for this paper is 100.  The marks for questions are shown in brackets.  You are expected to use a calculator where appropriate.  You are reminded of the need for good English and clear presentation in your answers. For Examiner’s Use Question Mark 1 2 3 4 5 6 7 8 TOTAL Please write clearly in block capitals. Centre number Candidate number Surname ________________________________________________________________________ Forename(s) ________________________________________________________________________ Candidate signature ________________________________________________________________________ I declare this is my own work. H 2 (02) G/Jun23/8465/2H Do not write outside the box 0 1 All living organisms are made of cells. Figure 1 shows two types of cell. Figure 1 28.6 micrometres Not to scale Cell B Liver cell 4.4 micrometres Bacterial cell Cell A 0 1 . 1 Calculate how many times longer the liver cell is than the bacterial cell. [2 marks] ___________________________________________________________________________________ ___________________________________________________________________________________ Number of times longer = ____________________________________ 3 (03) G/Jun23/8465/2H Do not write outside the box Turn over 8 0 1 . 2 Compare the structure of cell A with the structure of cell B. You should include similarities and differences in your answer. Do not refer to cell size. [4 marks] ___________________________________________________________________________________ ___________________________________________________________________________________ ___________________________________________________________________________________ ___________________________________________________________________________________ ___________________________________________________________________________________ ___________________________________________________________________________________ ___________________________________________________________________________________ ___________________________________________________________________________________ 0 1 . 3 In multicellular organisms, cells are organised into tissues. What is meant by a ‘tissue’? [1 mark] ___________________________________________________________________________________ ___________________________________________________________________________________ Question 1 continues on the next page 4 (04) G/Jun23/8465/2H Do not write outside the box A scientist investigated the effect of different concentrations of sugar solution on red blood cells. Figure 2 shows the effect of placing a red blood cell into a sugar solution. Figure 2 Red blood cell before being placed in sugar solution Red blood cell after being placed in sugar solution 0 1 . 4 What conclusion can be made from the result in Figure 2? [1 mark] Tick () one box. The sugar solution was less concentrated than inside the cell. The sugar solution was the same concentration as inside the cell. The sugar solution was more concentrated than inside the cell. 5 (05) G/Jun23/8465/2H Do not write outside the box Turn over 8 A student investigated the effect of different concentrations of sugar solution on the change in mass of plant tissue. The student used pieces of potato. 0 1 . 5 Describe a method the student could use to produce valid results. [6 marks] ___________________________________________________________________________________ ___________________________________________________________________________________ ___________________________________________________________________________________ ___________________________________________________________________________________ ___________________________________________________________________________________ ___________________________________________________________________________________ ___________________________________________________________________________________ ___________________________________________________________________________________ ___________________________________________________________________________________ ___________________________________________________________________________________ ___________________________________________________________________________________ ___________________________________________________________________________________ ___________________________________________________________________________________ ___________________________________________________________________________________ ___________________________________________________________________________________ Question 1 continues on the next page 6 (06) G/Jun23/8465/2H Do not write outside the box The student used a valid method. The student calculated the percentage change in mass of the pieces of potato. Table 1 shows the results. Table 1 Concentration of sugar solution in mol/dm3 Percentage (%) change in mass 0.0 28 0.1 15 0.2 3 0.3 –5 0.4 –10 0.5 –12 0 1 . 6 Complete Figure 3. You should:  plot the data from Table 1  draw a line of best fit. Some of the results have been plotted for you. [2 marks] 7 (07) G/Jun23/8465/2H Do not write outside the box Turn over 8 Figure 3 –10 –15 –5 10 20 30 5 15 25 Concentration of sugar solution in mol/dm3 Percentage (%) change in mass 0.0 0.1 0.2 0.3 0.4 0.5 0 0 1 . 7 Determine the concentration of sugar solution that would cause no change in the mass of a piece of the potato. Use Figure 3. [1 mark] Concentration of sugar solution = _________________________ mol/dm3 ____ 17 Turn over for the next question 8 (08) G/Jun23/8465/2H Do not write outside the box 0 2 A student investigated how the density of a liquid affects the position of a wooden block floating in the liquid. Figure 4 shows the apparatus. Figure 4 Wooden block Ruler 15 14 13 12 11 10 9 8 7 6 5 4 3 2 1 0 Liquid surface Liquid Beaker Bottom of block This is the method used. 1. Put the wooden block in the beaker of liquid. 2. Allow the wooden block to come to rest so that it is floating in the liquid. 3. Measure the distance between the liquid surface and the bottom of the block. 4. Repeat steps 1 to 3 with liquids of different densities. 0 2 . 1 Give the independent variable in the investigation. [1 mark] ___________________________________________________________________________________ ___________________________________________________________________________________ 9 (09) G/Jun23/8465/2H Do not write outside the box Turn over 8 0 2 . 2 Give one control variable for the investigation. [1 mark] ___________________________________________________________________________________ ___________________________________________________________________________________ 0 2 . 3 Give one possible source of error when the student measured the distance between the liquid surface and the bottom of the block. [1 mark] ___________________________________________________________________________________ ___________________________________________________________________________________ 0 2 . 4 Table 2 shows the results. Table 2 Liquid Density of liquid in g/cm3 Distance between liquid surface and bottom of the block in cm A 1.4 5.5 B 1.2 6.4 C 1.0 7.7 D 0.9 8.5 Give one conclusion from the results. [1 mark] ___________________________________________________________________________________ ___________________________________________________________________________________ Question 2 continues on the next page 10 G/Jun23/8465/2H Do not write outside the box (10) Use the Physics Equations Sheet to answer questions 02.5 and 02.6. 0 2 . 5 Which equation links density (ρ), mass (m) and volume (V)? [1 mark] Tick () one box. ρ = m × V ρ = m V ρ = m × V 3 ρ = V m 0 2 . 6 The density of the wooden block was 0.85 g/cm3. The mass of the wooden block was 30.6 g. Calculate the volume of the wooden block in cm3. [3 marks] ___________________________________________________________________________________ ___________________________________________________________________________________ ___________________________________________________________________________________ ___________________________________________________________________________________ ___________________________________________________________________________________ Volume of wooden block = _________________________ cm3 11 G/Jun23/8465/2H Do not write outside the box Turn over 8 (11) 0 2 . 7 Liquid C is water. When liquid water is heated to its boiling point the water changes state. What happens to the density of the liquid water as it changes state? [2 marks] Tick () one box. The density decreases The density stays the same The density increases Give a reason for your answer. ___________________________________________________________________________________ ___________________________________________________________________________________ ____ 10 Turn over for the next question 12 G/Jun23/8465/2H Do not write outside the box (12) 0 3 The small intestine (gut) is adapted to digest and absorb food molecules. Digested food molecules pass across the wall of the small intestine into the blood. Figure 5 shows part of the wall of the small intestine. Figure