BIOCHEM C785 Final Exam[with complete solutions]

BIOCHEM C785 Final Exam[with complete solutions] Which mRNA sequence results in a polypeptide that contains three amino acids? Correct answer- 5' - CUU GAU ACU UAG - 3' Which RNA sequence would end in the following polypeptide? Phe-Thr-Val-Stop Correct answer- 5' - UUC ACA GUA UAA - 3' What is the complementary sequence for the following DNA sequence? 5' - GCC ATC TCG AAT - 3' Correct answer- 5' - ATT CGA GAT GGC - 3' Assuming 100% reaction efficiency, how many DNA copies are created after the completion of four complete PCR cycles? Correct answer- 16 What is the function of DNA polymerase in the process of PCR? Correct answer- It recognizes the primers and uses the available dNTPs to replicate the template DNA sequence. 5' - [...] ATT CGC TAG GGC [...] - 3' − Normal DNA 5' - [...] ATT CGC GAG GGC [...] - 3' − Mutant DNA Which type of mutation and outcome characterizes the Mutant DNA? Correct answer- A missense mutation leading to a longer protein In sickle-cell disease, the glutamic acid (Glu) at position number seven is mutated to valine (Val). What change in codons corresponds to this mutation? Correct answer- GAA to GTA What are possible blood types of the parents of a patient with blood type AB? Correct answer- A and B Which pair of chromosomes accurately depicts the genotype of a person with an autosomal recessive mutant HBB allele and sickle cell anemia? Correct answer- option 3- 2 mutant genes on chromosome 11