Nester's Microbiology Ch 1-4 Multiple
choice
A method that counts only live microbes is the
A)pure culture method.
B)plate count method.
C)cell counting method.
D)turbidity method.
E)None of these choices are correct. - ANSB)plate count method.
Isolating a pure microbial culture from a mixed culture can be best accomplished by the
A)broth culture method.
B)streak plate method.
C)membrane filtration method.
D)multi-tube method.
E)None of these choices are correct. - ANSB)streak plate method.
The problem with using gelatin as a solidifying agent for microbiological media is that
A)many organisms can degrade gelatin.
B)gelatin may not remain solid at temperatures required for microbial growth.
C)gelatin is not stable during sterilization procedures.
D)gelatin liquefies at warm temperatures.
E)All of these choices are correct - ANSE)All of these choices are correct
A microorganism isolated from the Arctic region would most likely be
A)a mesophile.
B)facultative.
C)a psychrophile.
D)extremophilic.
E)a psychrotroph. - ANSC)a psychrophile.
A microorganism that grows in the presence of oxygen when it is available but can also grow
without oxygen is
A)microaerophilic.
B)aerobic.
C)an aerotolerant anaerobe.
D)a facultative anaerobe.
E)None of these choices are correct. - ANSD)a facultative anaerobe.
When organisms use oxygen in aerobic respiration, helpful derivatives called reactive oxygen
species form as by-products.
,A)True
B)False - ANSB)False
Organisms that require organic compounds for a carbon and energy source are called
A)chemolithoautotrophs.
B)chemoorganoheterotrophs.
C)mesotrophs.
D)psychrotrophs.
E)aerotrophs. - ANSB)chemoorganoheterotrophs.
Cobalt, zinc, copper and manganese are examples of _____________________________.
A)major elements
B)trace elements
C)vital proteins to a cell
D)growth factors
E)None of the above. - ANSB)trace elements
An organism is cultured in media containing hydrogen sulfide and carbon dioxide as the only
sources for energy and carbon. This would be
A)an enrichment media for chemoorganoheterotrophs.
B)a differential media for chemoorganoheterotrophs.
C)an enrichment media for chemolithoautotrophs.
D)a differential medium for photoheterotrophs.
E)None of these choices are correct - ANSC)an enrichment media for chemolithoautotrophs.
Which of the following statements is FALSE?
A)Bacteria growing in log phase are least affected by antibiotics.
B)The generation time of bacteria may vary from species to species.
C)Bacteria divide by binary fission, producing two daughter cells.
D)Secondary metabolites are normally produced during late log phase.
E)In the lag phase, cells increase the number of ribosomes they contain. - ANSA)Bacteria
growing in log phase are least affected by antibiotics.
Differential media contain an ingredient that certain microbes can change in a recognizable way.
A)True
B)False - ANSA)True
When the number of cells in a culture increases exponentially, the phase of the culture cycle is
termed
A)lag.
B)log.
C)stationary.
D)death.
E)prolonged decline. - ANSB)log.
, The growth phase in which cells are adapting to new growth conditions is termed
A)lag.
B)log.
C)stationary.
D)death.
E)prolonged decline. - ANSA)lag.
Selective media is used to isolate a specific organism or group of organisms.
A)True
B)False - ANSA)True
A chemostat is used to
A)select for certain species of bacteria.
B)enrich the culture environment for certain species of bacteria.
C)maintain a bacterial culture in continuous exponential growth.
D)maintain bacterial cultures for future generations.
E)None of these choices are correct. - ANSC)maintain a bacterial culture in continuous
exponential growth.
What are extracellular polymeric substances?
A)A method used for estimating the concentration of cells in a specimen.
B)A meshlike accumulation of polymers that give biofilms a slimy appearance.
C)A substance added to stock cultures to help maintain storage.
D)An ingredient used for complex media preparation.
E)A substance produced by halophiles when adapting to cold environments. - ANSB)A meshlike
accumulation of polymers that give biofilms a slimy appearance.
The Most Probable Number (MPN) method of determining cell numbers is based on
A)serial dilutions.
B)turbidity.
C)membrane filtration.
D)direct microscopic count.
E)cell-counting chamber. - ANSA)serial dilutions.
The majority of bacterial infections are linked to biofilms.
A)True
B)False - ANSA)True
During which phase(s) of culture growth are the number of newly formed cells approximately
equal to the number of dying cells?
A)stationary
B)lag
C)log
choice
A method that counts only live microbes is the
A)pure culture method.
B)plate count method.
C)cell counting method.
D)turbidity method.
E)None of these choices are correct. - ANSB)plate count method.
Isolating a pure microbial culture from a mixed culture can be best accomplished by the
A)broth culture method.
B)streak plate method.
C)membrane filtration method.
D)multi-tube method.
E)None of these choices are correct. - ANSB)streak plate method.
The problem with using gelatin as a solidifying agent for microbiological media is that
A)many organisms can degrade gelatin.
B)gelatin may not remain solid at temperatures required for microbial growth.
C)gelatin is not stable during sterilization procedures.
D)gelatin liquefies at warm temperatures.
E)All of these choices are correct - ANSE)All of these choices are correct
A microorganism isolated from the Arctic region would most likely be
A)a mesophile.
B)facultative.
C)a psychrophile.
D)extremophilic.
E)a psychrotroph. - ANSC)a psychrophile.
A microorganism that grows in the presence of oxygen when it is available but can also grow
without oxygen is
A)microaerophilic.
B)aerobic.
C)an aerotolerant anaerobe.
D)a facultative anaerobe.
E)None of these choices are correct. - ANSD)a facultative anaerobe.
When organisms use oxygen in aerobic respiration, helpful derivatives called reactive oxygen
species form as by-products.
,A)True
B)False - ANSB)False
Organisms that require organic compounds for a carbon and energy source are called
A)chemolithoautotrophs.
B)chemoorganoheterotrophs.
C)mesotrophs.
D)psychrotrophs.
E)aerotrophs. - ANSB)chemoorganoheterotrophs.
Cobalt, zinc, copper and manganese are examples of _____________________________.
A)major elements
B)trace elements
C)vital proteins to a cell
D)growth factors
E)None of the above. - ANSB)trace elements
An organism is cultured in media containing hydrogen sulfide and carbon dioxide as the only
sources for energy and carbon. This would be
A)an enrichment media for chemoorganoheterotrophs.
B)a differential media for chemoorganoheterotrophs.
C)an enrichment media for chemolithoautotrophs.
D)a differential medium for photoheterotrophs.
E)None of these choices are correct - ANSC)an enrichment media for chemolithoautotrophs.
Which of the following statements is FALSE?
A)Bacteria growing in log phase are least affected by antibiotics.
B)The generation time of bacteria may vary from species to species.
C)Bacteria divide by binary fission, producing two daughter cells.
D)Secondary metabolites are normally produced during late log phase.
E)In the lag phase, cells increase the number of ribosomes they contain. - ANSA)Bacteria
growing in log phase are least affected by antibiotics.
Differential media contain an ingredient that certain microbes can change in a recognizable way.
A)True
B)False - ANSA)True
When the number of cells in a culture increases exponentially, the phase of the culture cycle is
termed
A)lag.
B)log.
C)stationary.
D)death.
E)prolonged decline. - ANSB)log.
, The growth phase in which cells are adapting to new growth conditions is termed
A)lag.
B)log.
C)stationary.
D)death.
E)prolonged decline. - ANSA)lag.
Selective media is used to isolate a specific organism or group of organisms.
A)True
B)False - ANSA)True
A chemostat is used to
A)select for certain species of bacteria.
B)enrich the culture environment for certain species of bacteria.
C)maintain a bacterial culture in continuous exponential growth.
D)maintain bacterial cultures for future generations.
E)None of these choices are correct. - ANSC)maintain a bacterial culture in continuous
exponential growth.
What are extracellular polymeric substances?
A)A method used for estimating the concentration of cells in a specimen.
B)A meshlike accumulation of polymers that give biofilms a slimy appearance.
C)A substance added to stock cultures to help maintain storage.
D)An ingredient used for complex media preparation.
E)A substance produced by halophiles when adapting to cold environments. - ANSB)A meshlike
accumulation of polymers that give biofilms a slimy appearance.
The Most Probable Number (MPN) method of determining cell numbers is based on
A)serial dilutions.
B)turbidity.
C)membrane filtration.
D)direct microscopic count.
E)cell-counting chamber. - ANSA)serial dilutions.
The majority of bacterial infections are linked to biofilms.
A)True
B)False - ANSA)True
During which phase(s) of culture growth are the number of newly formed cells approximately
equal to the number of dying cells?
A)stationary
B)lag
C)log
