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Samenvatting medische bacteriologie 1 (2MLT): voedingsbodems, multitestmedia, biochemische testen, besproken bacteriën, antibiotica

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Samenvatting medische bacteriologie 1 (2MLT): voedingsbodems, multitestmedia, biochemische testen, besproken bacteriën, antibiotica - slides - cursus - notities les

Institution
Hogeschool Gent (HoGent)
Course
Medische Bacteriologie 1








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Institution
Hogeschool Gent (HoGent)
Study
Biomedische Laboratoriumtechnologie
Course
Medische Bacteriologie 1
All documents for this subject (21)

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Uploaded on
June 1, 2022
Number of pages
15
Written in
2021/2022
Type
Summary

Subjects

  • medische bacteriologie 1
  • mlt
  • hogent

Content preview

Overzicht belangrijke onderdelen medische
bacteriologie 1
Universele voedingsbodems

Rijke voedingsbodem door
Ongeënt: rood
toevoegen paarden – of
α-hemolyse: groene kleur
Bloedagar schapenbloed: omzetting
β-hemolyse: heldere zone
Hb tot metHb m.b.v.
ϓ- hemolyse: geen hemolyse
hemolysines
Sterk aangereikt medium
door vrijkomen nutriënten
Chocolade-
uit gelyseerde RBC: X- Ongeënt: bruin
agar
factor (hemin) en V-factor
(NAD+)
Thioglycollaat: groei Enkel bovenaan: strikt aeroob
TSB anaeroben door lage Overal: factultatief anaeroob
redoxpotentiaal Enkel onderaan: strikt anaeroob
Extract van hersen – en
BHI Ongeënt: geel
hartweefsel van varkens




Selectieve voedingsbodems

Galzouten en kristalviolet:
Ongeënt: rood
enkel groei gram –
MC Lactose +: rood
Lactose
Lactose -: beige
pH-indicator
Hoge zoutconcentratie:
Ongeënt: roze/rood
MSA enkel gram +
Mannitol +: geel
Mannitol
Galzouten: enkel gram –
Lactose
Ongeënt: groen
Sucrose
HEA Lactose +: oranje/roze
Ijzerammoniumcitraat
H2S: zwart centrum (FeS)
Cysteïne en thiosulfaat
pH-indicator
Eosinemethyleenblauw: Ongeënt: donker bruin
EMB enkel gram – Lactose +: donker roze/paars
Lactose Lactose -: kleurloos
Lactose
pH-indicator Ongeënt: blauw/groen
CLED Cystine Lactose +: geel
Laag elektrolytgehalte: Lactose -: blauw/kleurloos
geen uitzwerming
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