AND ANSWERS 2026
Which of the four elements in proteins differentiated this class of substances from
carbohydrates and lipids? - ANSWERSNitrogen
Which of the proteins is soluble in water? - ANSWERSAlbumin
How are amino acids joined together to form a chainlike structure? - ANSWERSPeptide
bonding
Proteins may become denatured when subjected to mechanical agitation, heat, or
chemical treatment. What does denaturation of proteins refer to? - ANSWERSAlteration
of tertiary structure
Which aminoacidopathy is NOT caused by an enzyme deficiency? -
ANSWERSCystinuria
These aminoacidopathies are caused by an enzyme deficiency: - ANSWERSMaple
syrup disease, phenylketonuria, and alkaptonuria
How many immunoglobulin classes are currently recognized? - ANSWERSFive
Which reagent is employed in the serum protein determination? - ANSWERSBiuret
Why should hemolyzed serum not be used for total protein analysis? -
ANSWERSHemoglobin is absorbed at the same wavelength as protein in the reagent
Which of the following is NOT true of the biuret reaction? - ANSWERSIt is relatively free
from interference from lipids and hemoglobin
Which of the following is true of the biuret reaction? - ANSWERSFollows Beer's law for
a reasonable range of protein, depends on the presence of at least two peptide
linkages, and is insensitive to the low protein levels of urine.
Why is bromcresol purple the preferred indicator for albumin dye binding techniques? -
ANSWERSThere is less interference from pigment(s)
What is the most anodic protein on electrophoresis at pH 8.6? - ANSWERSAlbumin
The total protein is 7.1 g/dL. The serum protein results are:
Albumin 53.0% 3.8 g/dL
Alpha1 3.6% 0.3 g/dL
, Alpha2 11.0% 0.8 g/dL
Beta 17.6% 1.2 g/dL
Gamma 14.8% 1.1 g/dL
What is the A/G ratio? - ANSWERS3.8/(.3+.8+1.2+1.1) = 1.1
A serum protein electrophoretic pattern displaying a beta gamma bridge or broad
gamma is seen in which disease state? - ANSWERSActive cirrhosis
If an electrophoretic pattern shows 30% albumin, 4%-10% alpha-globulin, 4%-10%
beta-globulin, and 45% gamma globulin, then what condition may exist in the patient? -
ANSWERSMonoclonal gammopathy
Glycoproteins and mucoproteins are usually bound to which substance? -
ANSWERSCarbohydrates
All of the following are glycoproteins: - ANSWERSCeruloplasmin, follicle-stimulating
hormone (FSH), and fibrinogen
All of the following are glycoproteins EXCEPT: - ANSWERSCryoglobulin
Which one of the following applies to cryoglobulins? - ANSWERSThey are temperature-
sensitive proteins
Which of the following statements is true of albumin? - ANSWERSIt is produced in the
liver and is water-soluble
Decreased alpha-fetoprotein values are associated with which condition? -
ANSWERSDown syndrome
Which one of the following proteins transports iron? - ANSWERSTransferrin
In acute renal failure, which nonprotein nitrogen rises the fastest? - ANSWERSBlood
urea nitrogen (BUN)
By the urease method, urea is enzymatically converted to which end product? -
ANSWERSAmmonia
Why can untreated urine be used for the determination of urea by the diacetyl
monoxomine method? - ANSWERSThe method is not measuring ammonia
An elevated creatinine value is most likely to be accompanied by which of these values?
- ANSWERSElevated BUN, 10X creatinine value
What is the classical method for creatinine reaction? - ANSWERSJaffe