1|Page
BIOTECH FINAL EXAM, MOST RECENT EXAM 2026
ACTUAL COMPLETE REAL EXAM QUESTIONS AND
CORRECT ANSWERS ||VERIFIED EXAM!!(VERIFIED
ANSWERS) ALREADY GRADED A+ / NEWEST EXAM!!!
Polyacrylamide gel electrophoresis - Answers-technique
designed for separating and analyzing proteins according
to their size
Alpha helices and Beta-pleated sheets represent the
_______ structure of a protein - Answers-Secondary
True/False?
Lactose is an enzyme - Answers-False
Adding a carbohydrate group to a protein is known as
_____. - Answers-glycosylation
Adding a phosphate or sugar group to a newly made
protein is an example of _____. - Answers-a
posttranslational modification
,2|Page
An advantage of using E.coli bacterial cells to express
proteins is that ______________. - Answers-they generate
unlimited quantities to proteins
A disadvantage of using bacterial cells to grow proteins is
that _____________. - Answers-they do not correctly fold
all proteins
Cells must be lysed to release the proteins of interest if
__________. - Answers-the protein is intracellular.
HPLC is _____________. - Answers-high-performance
liquid chromatography
When making a complementary DNA (cDNA) library, which
enzyme is used to copy mRNA into DNA? - Answers-
Reverse transcriptase
___________ is a DNA-binding dye that fluoresces when
DNA is an agarose gel illuminated with ultraviolet light. -
Answers-Ethidium bromide
,3|Page
Transformation in a cloning experiment is ___________. -
Answers-inserting DNA into bacteria cells
True/False?
Restriction enzymes create phosphodiester bond between
pieces of DNA in a cloning experiment. - Answers-False
Which of the following techniques is the best choice for
amplifying DNA? - Answers-PCR
Which of the following techniques is most commonly used
to separate and analyze DNA by size? - Answers-Agarose
gel elecrophoresis
A recombinant DNA molecule is produced artificially and
contains sequences __________. - Answers-from
unrelated organisms
During molecular cloning, a gene of interest (insulin) is
inserted into a bacterial structure called a ________ and
enters bacterial cell through a process called ______. -
Answers-plasmid; transformation
, 4|Page
Blocking with _____ increases the possibility of non
specific protein binding by the primary antibody - Answers-
milk
combing DNA from different sources is an example of -
Answers-recombinant DNA technology
______ is immunological assays commonly used to
measure antibodies, antigens, proteins and are generally
carried out in 96 well plates, allowing multiple sample to
be measured in a single experiment - Answers-ELISA
True/False?
In general, the primary antibody that recognizes the target
protein in a western blot is not directly detectable.
Therefore, specific tertiary antibodies are used as the
means of ultimately detecting the target antigen. -
Answers-False
______ involves farm-raising finfish, shellfish, algae, and
other aquatic organisms. - Answers-Aquaculture
BIOTECH FINAL EXAM, MOST RECENT EXAM 2026
ACTUAL COMPLETE REAL EXAM QUESTIONS AND
CORRECT ANSWERS ||VERIFIED EXAM!!(VERIFIED
ANSWERS) ALREADY GRADED A+ / NEWEST EXAM!!!
Polyacrylamide gel electrophoresis - Answers-technique
designed for separating and analyzing proteins according
to their size
Alpha helices and Beta-pleated sheets represent the
_______ structure of a protein - Answers-Secondary
True/False?
Lactose is an enzyme - Answers-False
Adding a carbohydrate group to a protein is known as
_____. - Answers-glycosylation
Adding a phosphate or sugar group to a newly made
protein is an example of _____. - Answers-a
posttranslational modification
,2|Page
An advantage of using E.coli bacterial cells to express
proteins is that ______________. - Answers-they generate
unlimited quantities to proteins
A disadvantage of using bacterial cells to grow proteins is
that _____________. - Answers-they do not correctly fold
all proteins
Cells must be lysed to release the proteins of interest if
__________. - Answers-the protein is intracellular.
HPLC is _____________. - Answers-high-performance
liquid chromatography
When making a complementary DNA (cDNA) library, which
enzyme is used to copy mRNA into DNA? - Answers-
Reverse transcriptase
___________ is a DNA-binding dye that fluoresces when
DNA is an agarose gel illuminated with ultraviolet light. -
Answers-Ethidium bromide
,3|Page
Transformation in a cloning experiment is ___________. -
Answers-inserting DNA into bacteria cells
True/False?
Restriction enzymes create phosphodiester bond between
pieces of DNA in a cloning experiment. - Answers-False
Which of the following techniques is the best choice for
amplifying DNA? - Answers-PCR
Which of the following techniques is most commonly used
to separate and analyze DNA by size? - Answers-Agarose
gel elecrophoresis
A recombinant DNA molecule is produced artificially and
contains sequences __________. - Answers-from
unrelated organisms
During molecular cloning, a gene of interest (insulin) is
inserted into a bacterial structure called a ________ and
enters bacterial cell through a process called ______. -
Answers-plasmid; transformation
, 4|Page
Blocking with _____ increases the possibility of non
specific protein binding by the primary antibody - Answers-
milk
combing DNA from different sources is an example of -
Answers-recombinant DNA technology
______ is immunological assays commonly used to
measure antibodies, antigens, proteins and are generally
carried out in 96 well plates, allowing multiple sample to
be measured in a single experiment - Answers-ELISA
True/False?
In general, the primary antibody that recognizes the target
protein in a western blot is not directly detectable.
Therefore, specific tertiary antibodies are used as the
means of ultimately detecting the target antigen. -
Answers-False
______ involves farm-raising finfish, shellfish, algae, and
other aquatic organisms. - Answers-Aquaculture
