Biochemistry 9th Edition Campbell - Test Bank

1. How do the molecules that play a role in living cells compare to those encountered in organic chemistry? a. They are the same, just operating in a different context. b. Biological molecules are organic molecules, but the similarity ends there. c. Biological molecules aren't similar to organic molecules at all. d. Biology isn't based on molecules at all, but a "vital force". ANSWER: a POINTS: 1 QUESTION TYPE: Multiple Choice HAS VARIABLES: False LEARNING OBJECTIVES: CAFA.BIOC.15.1 - New in 6e TOPICS: Basic Themes DATE CREATED: 12/23/2013 2:14 PM DATE MODIFIED: 12/23/2013 2:14 PM 2. Which of the following shows the correct order from most simple to most complex: a. atom, molecule, organelle, macromolecule b. molecule, atom, macromolecule, organelle c. tissue, cell, organ d. atom, macromolecule, tissue, organ ANSWER: d POINTS: 1 QUESTION TYPE: Multiple Choice HAS VARIABLES: False LEARNING OBJECTIVES: CAFA.BIOC.15.2 - Added in 8e TOPICS: Basic Themes DATE CREATED: 12/23/2013 2:14 PM DATE MODIFIED: 11/16/2016 1:13 AM 3. Which of the following best defines organic chemistry? a. The study of compounds contained in organisms. b. The study of compounds containing organs. c. The study of compounds containing carbon and hydrogen and their derivatives. d. The study of compounds containing elements other than carbon. ANSWER: c POINTS: 1 QUESTION TYPE: Multiple Choice HAS VARIABLES: False LEARNING OBJECTIVES: CAFA.BIOC.15.1 - New in 6e TOPICS: Chemical Foundations of Biochemistry DATE CREATED: 12/23/2013 2:14 PM DATE MODIFIED: 12/23/2013 2:14 PM 4. Which of the following is NOT one of the primary simple molecules that scientists believe must have ultimately led to creating living things? a. ammonia b. carbon dioxide c. hydrogen d. simple carbohydrates ANSWER: d POINTS: 1 QUESTION TYPE: Multiple Choice HAS VARIABLES: False LEARNING OBJECTIVES: CAFA.BIOC.15.3 - New in 8e TOPICS: Basic Themes DATE CREATED: 12/23/2013 2:14 PM DATE MODIFIED: 11/16/2016 12:07 AM 5. Which of the following was part of the vital force theory? a. The compounds found in living things are just like those found in the non-living world. b. The compounds found in living things are interesting, but can easily be produced in the laboratory. c. The compounds found in living things can not be produced in the laboratory. ANSWER: c POINTS: 1 QUESTION TYPE: Multiple Choice HAS VARIABLES: False LEARNING OBJECTIVES: CAFA.BIOC.15.1 - New in 6e TOPICS: Chemical Foundations of Biochemistry DATE CREATED: 12/23/2013 2:14 PM DATE MODIFIED: 12/23/2013 2:14 PM 6. The synthesis of urea from ammonium cyanate. a. was a critical component of the Miller-Urey experiment. b. requires a protein as a catalyst. c. helped dispel the vital force theory. d. supported the vital force theory. ANSWER: c POINTS: 1 QUESTION TYPE: Multiple Choice HAS VARIABLES: False TOPICS: Chemical Foundations of Biochemistry DATE CREATED: 12/23/2013 2:14 PM DATE MODIFIED: 12/23/2013 2:14 PM 7. Which of the following is NOT a Functional Group a. Amino group b. Protein c. Alcohol group d. Carbonyl group ANSWER: b POINTS: 1 QUESTION TYPE: Multiple Choice HAS VARIABLES: False LEARNING OBJECTIVES: CAFA.BIOC.15.4 - New in 7e TOPICS: Chemical Foundations of Biochemistry DATE CREATED: 12/23/2013 2:14 PM DATE MODIFIED: 12/23/2013 2:14 PM 8. Which of the following functional groups is specific to an alcohol? a. -NH b. -OH c. -C=O d. C=C e. O-P ANSWER: b POINTS: 1 QUESTION TYPE: Multiple Choice HAS VARIABLES: False LEARNING OBJECTIVES: CAFA.BIOC.15.3 - New in 8e TOPICS: Chemical Foundations of Biochemistry DATE CREATED: 12/23/2013 2:14 PM DATE MODIFIED: 11/23/2016 12:42 AM 9. Which of the following functional groups are not commonly seen in biomolecules? a. Alkyl halides b. Amides c. Carboxylic acids d. Ethers e. Phosphate esters ANSWER: a POINTS: 1 QUESTION TYPE: Multiple Choice HAS VARIABLES: False LEARNING OBJECTIVES: CAFA.BIOC.15.5 - Modified from 5e TOPICS: Chemical Foundations of Biochemistry DATE CREATED: 12/23/2013 2:14 PM DATE MODIFIED: 12/23/2013 2:14 PM 10. Which of the following statements regarding biomolecules is false? a. They contain predominantly ionic bonds. b. They contain predominantly nonmetallic elements. c. Carbon is the key element. d. Specific stereoisomers are essential in most cases. ANSWER: a POINTS: 1 QUESTION TYPE: Multiple Choice HAS VARIABLES: False LEARNING OBJECTIVES: CAFA.BIOC.15.5 - Modified from 5e TOPICS: Chemical Foundations of Biochemistry DATE CREATED: 12/23/2013 2:14 PM DATE MODIFIED: 12/23/2013 2:14 PM 11. All of the following bonds are important in biomolecules, except: a. C−Cl b. C−H c. C−N d. O−H e. O−P ANSWER: a POINTS: 1 QUESTION TYPE: Multiple Choice HAS VARIABLES: False TOPICS: Chemical Foundations of Biochemistry DATE CREATED: 12/23/2013 2:14 PM DATE MODIFIED: 12/23/2013 2:14 PM 12. Which of the following statements regarding biopolymers is false? a. Different sequences of the monomers can lead to different functions. b. Only soluble polymers can be created from soluble monomers. c. A wide, almost uncountable variety of polymers can be created from just a few monomers. d. Different linkages between the monomers can lead to different functions. e. Biopolymers can fold up into complex shapes. ANSWER: b POINTS: 1 QUESTION TYPE: Multiple Choice HAS VARIABLES: False LEARNING OBJECTIVES: CAFA.BIOC.15.5 - Modified from 5e TOPICS: Chemical Foundations of Biochemistry DATE CREATED: 12/23/2013 2:14 PM DATE MODIFIED: 12/23/2013 2:14 PM 13. Which statement is not correct about peptide nucleic acids, PNA? a. They are combinations of peptides and nucleic acids. b. Scientists create them to study the origins of life c. They were proven to be the first hereditary molecule. d. They may combine the catalytic properties of proteins with the information transfer ability of nucleic acid e. All of these statements apply to PNA. ANSWER: c POINTS: 1 QUESTION TYPE: Multiple Choice HAS VARIABLES: False LEARNING OBJECTIVES: CAFA.BIOC.15.4 - New in 7e TOPICS: Chemical Foundations of Biochemistry DATE CREATED: 12/23/2013 2:14 PM DATE MODIFIED: 12/23/2013 2:14 PM 14. According to the big bang theory of the creation of the universe, a. the universe has been getting cooler since its beginning b. the initial explosion caused the creation of all of the elements of the periodic table c. carbon is the most abundant element in the universe d. the earth could be no older than 1 billion years ANSWER: a POINTS: 1 QUESTION TYPE: Multiple Choice HAS VARIABLES: False LEARNING OBJECTIVES: CAFA.BIOC.15.4 - New in 7e TOPICS: Origin of Life DATE CREATED: 12/23/2013 2:14 PM DATE MODIFIED: 12/23/2013 2:14 PM 15. In its earliest stages, which atoms were present in the universe? a. carbon, hydrogen, and oxygen b. hydrogen, helium, and lithium c. nitrogen, sulfur, and phosphorous d. uranium, polonium, and radium e. helium, neon, and argon ANSWER: b POINTS: 1 QUESTION TYPE: Multiple Choice HAS VARIABLES: False LEARNING OBJECTIVES: CAFA.BIOC.15.1 - New in 6e TOPICS: Origin of Life DATE CREATED: 12/23/2013 2:14 PM DATE MODIFIED: 12/23/2013 2:14 PM 16. How are the majority of elements thought to have been formed? a. By thermonuclear reactions that normally take place in stars. b. In explosions of stars. c. By the action of cosmic rays outside the stars since the formation of the galaxy. d. All of the choices are true e. None of the choices; all the elements were present from the initial Big Bang. ANSWER: d POINTS: 1 QUESTION TYPE: Multiple Choice HAS VARIABLES: False LEARNING OBJECTIVES: CAFA.BIOC.15.1 - New in 6e TOPICS: Origin of Life DATE CREATED: 12/23/2013 2:14 PM DATE MODIFIED: 12/23/2013 2:14 PM 17. What is the chemical formula for ozone? a. O2 b. O3 c. NH3 d. H2S e. CH4 ANSWER: b POINTS: 1 QUESTION TYPE: Multiple Choice HAS VARIABLES: False LEARNING OBJECTIVES: CAFA.BIOC.15.1 - New in 6e TOPICS: Origin of Life DATE CREATED: 12/23/2013 2:14 PM DATE MODIFIED: 12/23/2013 2:14 PM 18. It is generally believed that the following gas was missing in the primordial atmosphere: a. H2 b. CO2 c. CH4 d. NH3 e. O2 ANSWER: e POINTS: 1 QUESTION TYPE: Multiple Choice HAS VARIABLES: False TOPICS: Origin of Life DATE CREATED: 12/23/2013 2:14 PM DATE MODIFIED: 12/23/2013 2:14 PM 19. A catalyst a. increases the rate of a chemical reaction b. increases the amount of product obtained in a chemical reaction c. decreases the amount of product obtained in a chemical reaction d. none of the choices ANSWER: a POINTS: 1 QUESTION TYPE: Multiple Choice HAS VARIABLES: False TOPICS: Origin of Life DATE CREATED: 12/23/2013 2:14 PM DATE MODIFIED: 12/23/2013 2:14 PM 20. The genetic coding material is a. protein b. DNA c. polysaccharide d. lipid ANSWER: b POINTS: 1 QUESTION TYPE: Multiple Choice HAS VARIABLES: False TOPICS: Origin of Life DATE CREATED: 12/23/2013 2:14 PM DATE MODIFIED: 12/23/2013 2:14 PM 21. The presence of two anhydride linkages is an important feature of a. ATP b. proteins c. glucose d. carbon monoxide ANSWER: a POINTS: 1 QUESTION TYPE: Multiple Choice HAS VARIABLES: False LEARNING OBJECTIVES: CAFA.BIOC.15.3 - New in 8e TOPICS: Origins of Life DATE CREATED: 12/23/2013 2:14 PM DATE MODIFIED: 11/16/2016 1:16 AM 22. Which of the following best describes the results of the Miller-Urey experiment? a. It proved that DNA is the genetic material. b. It produced proteins under conditions simulating the early Earth. c. It created living cells from non-living materials. d. It produced some simple organic compounds from a mixture of gases presumed to have existed in the early atmosphere. e. All of these results of the Miller-Urey experiment. ANSWER: d POINTS: 1 QUESTION TYPE: Multiple Choice HAS VARIABLES: False LEARNING OBJECTIVES: CAFA.BIOC.15.1 - New in 6e TOPICS: Origin of Life DATE CREATED: 12/23/2013 2:14 PM DATE MODIFIED: 12/23/2013 2:14 PM 23. The idea that a coding system and a catalysis system came about separately and then combined to form life as we know it is known as a. the origin of life b. the big bang theory c. the double origen theory d. the theory of evolution ANSWER: c POINTS: 1 QUESTION TYPE: Multiple Choice HAS VARIABLES: False LEARNING OBJECTIVES: CAFA.BIOC.15.3 - New in 8e TOPICS: Origens of Life DATE CREATED: 12/23/2013 2:14 PM DATE MODIFIED: 11/16/2016 1:16 AM 24. The genetic code a. determines the order of sugars in a polysaccharide b. has no effect on the sequence of amino acids in proteins c. is the means by which the "blueprint" for living organisms is passed from one generation to the next d. cannot be understood by currently available experimental methods ANSWER: c POINTS: 1 QUESTION TYPE: Multiple Choice HAS VARIABLES: False TOPICS: Origin of Life DATE CREATED: 12/23/2013 2:14 PM DATE MODIFIED: 12/23/2013 2:14 PM 25. Biological catalysts are a. proteins exclusively b. RNA exclusively c. DNA exclusively d. some proteins and some RNA ANSWER: d POINTS: 1 QUESTION TYPE: Multiple Choice HAS VARIABLES: False TOPICS: Origin of Life DATE CREATED: 12/23/2013 2:14 PM DATE MODIFIED: 12/23/2013 2:14 PM 26. The main difference between prokaryotic and eukaryotic cells is the existence of _____ in eukaryotes. a. the nucleus b. ribosomes c. DNA d. RNA e. cell walls ANSWER: a POINTS: 1 QUESTION TYPE: Multiple Choice HAS VARIABLES: False LEARNING OBJECTIVES: CAFA.BIOC.15.5 - Modified from 5e TOPICS: Prokaryotes & Eukaryotes DATE CREATED: 12/23/2013 2:14 PM DATE MODIFIED: 11/29/2016 6:44 AM 27. All of the following features are common to all living organisms, except: a. Biomolecules b. Metabolic pathways c. Cellular structures d. DNA sequences e. RNA molecules ANSWER: c POINTS: 1 QUESTION TYPE: Multiple Choice HAS VARIABLES: False TOPICS: Prokaryotes & Eukaryotes DATE CREATED: 12/23/2013 2:14 PM DATE MODIFIED: 12/23/2013 2:14 PM 28. All eukaryotic organisms a. are multicellular b. have a nucleus c. have chloroplasts d. have a cell wall ANSWER: b POINTS: 1 QUESTION TYPE: Multiple Choice HAS VARIABLES: False LEARNING OBJECTIVES: CAFA.BIOC.15.4 - New in 7e TOPICS: Prokaryotes & Eukaryotes DATE CREATED: 12/23/2013 2:14 PM DATE MODIFIED: 12/23/2013 2:14 PM 29. Cell membranes a. are found in plants, but not in animals b. consist mainly of sugars c. do not allow transport into or out of the cell d. separate the cell from the outside world ANSWER: d POINTS: 1 QUESTION TYPE: Multiple Choice HAS VARIABLES: False TOPICS: Prokaryotes & Eukaryotes DATE CREATED: 12/23/2013 2:14 PM DATE MODIFIED: 12/23/2013 2:14 PM 30. Which of the following is not a subcellular organelle? a. nucleus b. mitochondrion c. endoplasmic reticulum d. cytoskeleton ANSWER: d POINTS: 1 QUESTION TYPE: Multiple Choice HAS VARIABLES: False LEARNING OBJECTIVES: CAFA.BIOC.15.4 - New in 7e TOPICS: Prokaryotes & Eukaryotes DATE CREATED: 12/23/2013 2:14 PM DATE MODIFIED: 12/23/2013 2:14 PM 31. Energy-yielding oxidation reactions take place in eukaryotic a. nuclei. b. ribosomes. c. mitochondria. d. endoplasmic reticula. e. cell walls. ANSWER: c POINTS: 1 QUESTION TYPE: Multiple Choice HAS VARIABLES: False LEARNING OBJECTIVES: CAFA.BIOC.15.1 - New in 6e TOPICS: Prokaryotes & Eukaryotes DATE CREATED: 12/23/2013 2:14 PM DATE MODIFIED: 12/23/2013 2:14 PM 32. Prokaryotic cells a. do not have a well defined nucleus b. are smaller than eukaryotic cells c. do not have internal membranes d. all of the above ANSWER: d POINTS: 1 QUESTION TYPE: Multiple Choice HAS VARIABLES: False TOPICS: Prokaryotic Structure DATE CREATED: 12/23/2013 2:14 PM DATE MODIFIED: 12/23/2013 2:14 PM 33. Prokaryotes a. contain ribosomes b. do not have a cell membrane c. contain mitochondria d. none of the above ANSWER: a POINTS: 1 QUESTION TYPE: Multiple Choice HAS VARIABLES: False TOPICS: Prokaryotic Structure DATE CREATED: 12/23/2013 2:14 PM DATE MODIFIED: 12/23/2013 2:14 PM 34. Ribosomes a. are the site of photosynthesis b. are the site of protein synthesis c. are never bound to membranes d. cannot be seen in the electron microscope ANSWER: b POINTS: 1 QUESTION TYPE: Multiple Choice HAS VARIABLES: False TOPICS: Prokaryotic Structure DATE CREATED: 12/23/2013 2:14 PM DATE MODIFIED: 12/23/2013 2:14 PM 35. 11 Ribosomes are made up of a. RNA and proteins b. DNA and proteins c. RNA and DNA d. proteins and carbohydrates ANSWER: a POINTS: 1 QUESTION TYPE: Multiple Choice HAS VARIABLES: False TOPICS: Prokaryotic Structure DATE CREATED: 12/23/2013 2:14 PM DATE MODIFIED: 12/23/2013 2:14 PM 36. Which of the following cellular components is commonly found in bacteria? a. Nucleus b. Ribosomes c. Chloroplasts d. Mitochondria e. More than one of these is characteristic of bacteria. ANSWER: b POINTS: 1 QUESTION TYPE: Multiple Choice HAS VARIABLES: False LEARNING OBJECTIVES: CAFA.BIOC.15.5 - Modified from 5e TOPICS: Prokaryotic Structure DATE CREATED: 12/23/2013 2:14 PM DATE MODIFIED: 12/23/2013 2:14 PM 37. Which organelle does not contain DNA? a. Nucleus b. Mitochondrion c. Rough Endoplasmic Reticulum d. Chloroplast e. All of these organelles contain DNA ANSWER: c POINTS: 1 QUESTION TYPE: Multiple Choice HAS VARIABLES: False TOPICS: Eukaryotic Structure DATE CREATED: 12/23/2013 2:14 PM DATE MODIFIED: 12/23/2013 2:14 PM 38. Which cell component is composed of RNA and protein? a. Nucleus b. Mitochondrion c. Endoplasmic Reticulum d. Chloroplast e. Ribosome ANSWER: e POINTS: 1 QUESTION TYPE: Multiple Choice HAS VARIABLES: False LEARNING OBJECTIVES: CAFA.BIOC.15.1 - New in 6e TOPICS: Eukaryotic Structure DATE CREATED: 12/23/2013 2:14 PM DATE MODIFIED: 12/23/2013 2:14 PM 39. Which cell component has cristae? a. Nucleus b. Mitochondrion c. Endoplasmic Reticulum d. Chloroplast e. Ribosome ANSWER: b POINTS: 1 QUESTION TYPE: Multiple Choice HAS VARIABLES: False LEARNING OBJECTIVES: CAFA.BIOC.15.1 - New in 6e TOPICS: Eukaryotic Structure DATE CREATED: 12/23/2013 2:14 PM DATE MODIFIED: 12/23/2013 2:14 PM 40. Which organelle is involved in the synthesis of ATP? a. Nucleus b. Mitochondrion c. Chloroplast d. ATP is synthesized in both mitochondria and chloroplasts. e. ATP is synthesized in all three organelles. ANSWER: d POINTS: 1 QUESTION TYPE: Multiple Choice HAS VARIABLES: False TOPICS: Eukaryotic Structure DATE CREATED: 12/23/2013 2:14 PM DATE MODIFIED: 12/23/2013 2:14 PM 41. Eukaryotic DNA a. is found in the nucleus b. is found in the mitochondrion c. is found in the chloroplast d. all of the above ANSWER: d POINTS: 1 QUESTION TYPE: Multiple Choice HAS VARIABLES: False TOPICS: Eukaryotic Structure DATE CREATED: 12/23/2013 2:14 PM DATE MODIFIED: 12/23/2013 2:14 PM 42. Which of the following statements about eukaryotic nuclei is FALSE? a. They are separated from the rest of the cell by a single membrane. b. They contain RNA. c. They contain chromatin. d. They play a role in genetics. ANSWER: a POINTS: 1 QUESTION TYPE: Multiple Choice HAS VARIABLES: False LEARNING OBJECTIVES: CAFA.BIOC.15.5 - Modified from 5e TOPICS: Eukaryotic Structure DATE CREATED: 12/23/2013 2:14 PM DATE MODIFIED: 12/23/2013 2:14 PM 43. Which cell component does not have a double membrane? a. Nucleus b. Lysosome c. Rough Endoplasmic Reticulum d. Chloroplast e. Mitochondrion ANSWER: b POINTS: 1 QUESTION TYPE: Multiple Choice HAS VARIABLES: False LEARNING OBJECTIVES: CAFA.BIOC.15.5 - Modified from 5e TOPICS: Eukaryotic Structure DATE CREATED: 12/23/2013 2:14 PM DATE MODIFIED: 12/23/2013 2:14 PM 44. Which of the following statements about eukaryotic mitochondria is TRUE? a. They play a role in genetics. b. They are the site of photosynthesis in green plants. c. They have an inner and an outer membrane. d. They only occur in animals, not plants. ANSWER: c POINTS: 1 QUESTION TYPE: Multiple Choice HAS VARIABLES: False LEARNING OBJECTIVES: CAFA.BIOC.15.5 - Modified from 5e TOPICS: Eukaryotic Structure DATE CREATED: 12/23/2013 2:14 PM DATE MODIFIED: 12/23/2013 2:14 PM 45. Which is not a property of ribosomes? a. They are an assembly of polypeptides and RNA. b. They are found in both prokaryotic and eukaryotic cells. c. They function as agents in the biosynthesis of proteins. d. They are found in the cytoplasm and smooth endoplasmic reticulum. e. All of these statements are true about ribosomes. ANSWER: d POINTS: 1 QUESTION TYPE: Multiple Choice HAS VARIABLES: False TOPICS: Eukaryotic Structure DATE CREATED: 12/23/2013 2:14 PM DATE MODIFIED: 12/23/2013 2:14 PM 46. Which cell component is able to capture the energy of light? a. Nucleus b. Lysosome c. Rough Endoplasmic Reticulum d. Chloroplast e. Mitochondrion ANSWER: d POINTS: 1 QUESTION TYPE: Multiple Choice HAS VARIABLES: False LEARNING OBJECTIVES: CAFA.BIOC.15.5 - Modified from 5e TOPICS: Eukaryotic Structure DATE CREATED: 12/23/2013 2:14 PM DATE MODIFIED: 12/23/2013 2:14 PM 47. Which cell component contains many hydrolytic enzymes? a. Nucleus b. Lysosome c. Rough Endoplasmic Reticulum d. Chloroplast e. Mitochondrion ANSWER: b POINTS: 1 QUESTION TYPE: Multiple Choice HAS VARIABLES: False LEARNING OBJECTIVES: CAFA.BIOC.15.5 - Modified from 5e TOPICS: Eukaryotic Structure DATE CREATED: 12/23/2013 2:14 PM DATE MODIFIED: 12/23/2013 2:14 PM 48. The following cellular component is characteristic of eukaryotic cells: a. Nucleus b. Ribosomes c. Chloroplasts d. Mitochondria e. More than one of these is characteristic of eukaryotic cells. ANSWER: e POINTS: 1 QUESTION TYPE: Multiple Choice HAS VARIABLES: False TOPICS: Eukaryotic Structure DATE CREATED: 12/23/2013 2:14 PM DATE MODIFIED: 12/23/2013 2:14 PM 49. The following cellular component is the defining component of eukaryotic cells: a. Nucleus b. Ribosomes c. Chloroplasts d. Mitochondria e. Cell membranes ANSWER: a POINTS: 1 QUESTION TYPE: Multiple Choice HAS VARIABLES: False TOPICS: Eukaryotic Structure DATE CREATED: 12/23/2013 2:14 PM DATE MODIFIED: 12/23/2013 2:14 PM 50. The mitochondrial matrix a. is the location of enzymes needed for oxidation reactions b. contains an array of microtubules c. is part of the endoplasmic reticulum d. lies between the inner and outer mitochondrial membrane ANSWER: a POINTS: 1 QUESTION TYPE: Multiple Choice HAS VARIABLES: False TOPICS: Eukaryotic Structure DATE CREATED: 12/23/2013 2:14 PM DATE MODIFIED: 12/23/2013 2:14 PM 51. The following cellular component is the defining component of most plant cells: a. Nucleus b. Ribosomes c. Chloroplasts d. Mitochondria e. Cell walls ANSWER: c POINTS: 1 QUESTION TYPE: Multiple Choice HAS VARIABLES: False TOPICS: Eukaryotic Structure DATE CREATED: 12/23/2013 2:14 PM DATE MODIFIED: 12/23/2013 2:14 PM 52. The endoplasmic reticulum a. is part of a continuous membrane system throughout the cell b. occurs in two forms, rough and smooth c. can have ribosomes bound to it d. all of the above ANSWER: d POINTS: 1 QUESTION TYPE: Multiple Choice HAS VARIABLES: False TOPICS: Eukaryotic Structure DATE CREATED: 12/23/2013 2:14 PM DATE MODIFIED: 12/23/2013 2:14 PM 53. Chloroplasts a. contain no DNA b. are bounded by a single membrane c. are relatively small organelles d. are the site of photosynthesis in green plants ANSWER: d POINTS: 1 QUESTION TYPE: Multiple Choice HAS VARIABLES: False TOPICS: Eukaryotic Structure DATE CREATED: 12/23/2013 2:14 PM DATE MODIFIED: 12/23/2013 2:14 PM 54. The Golgi apparatus a. occurs in prokaryotes b. is involved in secretion of proteins from the cell c. is part of the chloroplast d. is the site of protein synthesis ANSWER: b POINTS: 1 QUESTION TYPE: Multiple Choice HAS VARIABLES: False TOPICS: Eukaryotic Structure DATE CREATED: 12/23/2013 2:14 PM DATE MODIFIED: 12/23/2013 2:14 PM 55. Lysosomes, peroxisomes, and glyoxysomes are a. sites of cell damage b. important in mitosis c. specialized organelles d. a part of the rough endoplasmic reticulum ANSWER: c POINTS: 1 QUESTION TYPE: Multiple Choice HAS VARIABLES: False TOPICS: Eukaryotic Structure DATE CREATED: 12/23/2013 2:14 PM DATE MODIFIED: 12/23/2013 2:14 PM 56. Cell walls a. occur in plants and bacteria b. occur in plants and animals c. occur only in plants d. occur only in bacteria ANSWER: a POINTS: 1 QUESTION TYPE: Multiple Choice HAS VARIABLES: False TOPICS: Eukaryotic Structure DATE CREATED: 12/23/2013 2:14 PM DATE MODIFIED: 12/23/2013 2:14 PM 57. Animal cells do not contain a. a nucleus b. mitochondria c. chloroplasts d. lysosomes ANSWER: c POINTS: 1 QUESTION TYPE: Multiple Choice HAS VARIABLES: False TOPICS: Eukaryotic Structure DATE CREATED: 12/23/2013 2:14 PM DATE MODIFIED: 12/23/2013 2:14 PM 58. A kind of cellular structure present in plant cells but not in human cells is a. the endoplasmic reticulum b. a cell wall c. ribosomes d. a plasma membrane ANSWER: b POINTS: 1 QUESTION TYPE: Multiple Choice HAS VARIABLES: False LEARNING OBJECTIVES: CAFA.BIOC.15.5 - Modified from 5e TOPICS: Eukaryotic Structure DATE CREATED: 12/23/2013 2:14 PM DATE MODIFIED: 12/23/2013 2:14 PM 59. Which of the following organelles does not have a double membrane? a. mitochondrion b. nucleus c. endoplasmic reticulum d. chloroplast ANSWER: c POINTS: 1 QUESTION TYPE: Multiple Choice HAS VARIABLES: False LEARNING OBJECTIVES: CAFA.BIOC.15.5 - Modified from 5e TOPICS: Eukaryotic Structure DATE CREATED: 12/23/2013 2:14 PM DATE MODIFIED: 12/23/2013 2:14 PM 60. Which of these kingdoms includes only prokaryotic organisms? a. Animals b. Fungi c. Monera d. Plants e. Protista ANSWER: c POINTS: 1 QUESTION TYPE: Multiple Choice HAS VARIABLES: False LEARNING OBJECTIVES: CAFA.BIOC.15.5 - Modified from 5e TOPICS: How we classify eukaryotes and prokaryotes DATE CREATED: 12/23/2013 2:14 PM DATE MODIFIED: 12/23/2013 2:14 PM 61. Which of these eukaryotic kingdoms consists primarily of unicellular organisms? a. Animals b. Fungi c. Plants d. Protista e. Both fungi and protista. ANSWER: d POINTS: 1 QUESTION TYPE: Multiple Choice HAS VARIABLES: False LEARNING OBJECTIVES: CAFA.BIOC.15.5 - Modified from 5e TOPICS: How we classify eukaryotes and prokaryotes DATE CREATED: 12/23/2013 2:14 PM DATE MODIFIED: 12/23/2013 2:14 PM 62. In the Five Kingdom classification system, human beings would be considered a. animals. b. protists. c. monera. d. fungi. e. none of the above. ANSWER: a POINTS: 1 QUESTION TYPE: Multiple Choice HAS VARIABLES: False LEARNING OBJECTIVES: CAFA.BIOC.15.5 - Modified from 5e TOPICS: How we classify eukaryotes and prokaryotes DATE CREATED: 12/23/2013 2:14 PM DATE MODIFIED: 12/23/2013 2:14 PM 63. In the Five Kingdom classification system, Escherichia coli would be considered a. animals. b. protists. c. monera. d. none of the above. ANSWER: c POINTS: 1 QUESTION TYPE: Multiple Choice HAS VARIABLES: False LEARNING OBJECTIVES: CAFA.BIOC.15.5 - Modified from 5e TOPICS: How we classify eukaryotes and prokaryotes DATE CREATED: 12/23/2013 2:14 PM DATE MODIFIED: 12/23/2013 2:14 PM 64. The endosymbiotic theory describes the origin of a. the nucleus & ribosomes. b. the Golgi and endoplasmic reticulum. c. lysosomes and the cytoskeleton. d. mitochondria & chloroplasts. ANSWER: d POINTS: 1 QUESTION TYPE: Multiple Choice HAS VARIABLES: False TOPICS: How we classify eukaryotes and prokaryotes DATE CREATED: 12/23/2013 2:14 PM DATE MODIFIED: 12/23/2013 2:14 PM 65. Which of following provides evidence for the endosymbiotic theory describing the origin of mitochondria & chloroplasts? a. These organelles have their own nuclei. b. These organelles have their own endoplasmic reticulum. c. These organelles have their own lysosomes. d. These organelles have their own DNA. ANSWER: d POINTS: 1 QUESTION TYPE: Multiple Choice HAS VARIABLES: False LEARNING OBJECTIVES: CAFA.BIOC.15.1 - New in 6e TOPICS: How we classify eukaryotes and prokaryotes DATE CREATED: 12/23/2013 2:14 PM DATE MODIFIED: 12/23/2013 2:14 PM 66. According to thermodynamics, favored processes are a. ones that require energy. b. ones that release energy. c. oxidations. d. reductions. ANSWER: b POINTS: 1 QUESTION TYPE: Multiple Choice HAS VARIABLES: False TOPICS: Biochemical Energetics DATE CREATED: 12/23/2013 2:14 PM DATE MODIFIED: 12/23/2013 2:14 PM 67. Which of the following is/are true? a. The hydrolysis of ATP releases energy. b. Favorable reactions are always fast. c. The hydrolysis of ATP requires the input of oxygen d. The hydrolysis of ATP yields more energy per molecule than the reaction of any other compound ANSWER: a POINTS: 1 QUESTION TYPE: Multiple Choice HAS VARIABLES: False LEARNING OBJECTIVES: CAFA.BIOC.15.4 - New in 7e TOPICS: Biochemical Energetics DATE CREATED: 12/23/2013 2:14 PM DATE MODIFIED: 12/23/2013 2:14 PM 68. The aerobic combustion of glucose to yield carbon dioxide and water a. is thermodynamically favorable b. requires oxygen c. has a negative Gibb’s free energy d. all of these are true ANSWER: d POINTS: 1 QUESTION TYPE: Multiple Choice HAS VARIABLES: False LEARNING OBJECTIVES: CAFA.BIOC.15.4 - New in 7e TOPICS: Energy and Change DATE CREATED: 12/23/2013 2:14 PM DATE MODIFIED: 12/23/2013 2:14 PM 69. A spontaneous reaction is a. exergonic. b. endergonic. c. at equilibrium. d. none of the above. ANSWER: a POINTS: 1 QUESTION TYPE: Multiple Choice HAS VARIABLES: False LEARNING OBJECTIVES: CAFA.BIOC.15.6 - Modified in 7e TOPICS: Spontaneity DATE CREATED: 12/23/2013 2:14 PM DATE MODIFIED: 12/23/2013 2:14 PM 70. The heat of a reaction at constant pressure is a. its change in entropy. b. its change in enthalpy. c. its change in free energy. d. its spontaneity. ANSWER: b POINTS: 1 QUESTION TYPE: Multiple Choice HAS VARIABLES: False TOPICS: Life and Thermodynamics DATE CREATED: 12/23/2013 2:14 PM DATE MODIFIED: 12/23/2013 2:14 PM Match the macromolecules with the monomeric unit in each. a. nucleic acids b. proteins c. carbohydrate QUESTION TYPE: Matching HAS VARIABLES: False TOPICS: Origin of Life DATE CREATED: 12/23/2013 2:14 PM DATE MODIFIED: 12/23/2013 2:14 PM 71. amino acid ANSWER: b POINTS: 1 72. monosaccharide ANSWER: c POINTS: 1 73. nucleotide ANSWER: a POINTS: 1 74. Identify the class of basic biomolecules represented by the following structure. a. Carbohydrates b. Amino acids c. Nucleotides d. Lipids ANSWER: b POINTS: 1 QUESTION TYPE: Multiple Choice HAS VARIABLES: False TOPICS: Chemical Foundations of Biochemistry DATE CREATED: 11/25/2016 4:56 AM DATE MODIFIED: 11/25/2016 5:00 AM 75. Explain the significance of functional groups in biochemistry. ANSWER: Biomolecules have characteristic functional groups that determine their reactions. Many of these functional groups contain oxygen and nitrogen, which are among the most electronegative elements. As a result, many of these functional groups are polar, and their polar nature plays a crucial role in their reactivity. POINTS: 1 QUESTION TYPE: Essay HAS VARIABLES: False TOPICS: Chemical Foundations of Biochemistry DATE CREATED: 11/25/2016 5:01 AM DATE MODIFIED: 11/25/2016 5:31 AM 76. Which of the following biomolecules forms the molecular currency of the cell, adenosine triphosphate (ATP)? a. Nucleotides b. Esters c. Amino acids d. Lipids ANSWER: a POINTS: 1 QUESTION TYPE: Multiple Choice HAS VARIABLES: False TOPICS: Chemical Foundations of Biochemistry DATE CREATED: 11/25/2016 5:23 AM DATE MODIFIED: 11/25/2016 5:30 AM 77. Carbohydrates can be represented by a general formula of _____. a. R-CHn(NH2)-COOH b. CH3(CH2)nCO2H c. CnH2n-2 d. (CH2O)n ANSWER: d POINTS: 1 QUESTION TYPE: Multiple Choice HAS VARIABLES: False TOPICS: Chemical Foundations of Biochemistry DATE CREATED: 11/25/2016 5:32 AM DATE MODIFIED: 11/25/2016 5:34 AM 78. Lipids are characterized by poor solubility in water most of their structure is composed of _____. a. a central carbon atom bonded to a carboxyl group, a hydrogen group, and a variable group, called the R group b. a five-carbon sugar, a nitrogen-containing ring, and one or more phosphate groups c. long chains of hydrocarbons d. straight sugar chains that forms cyclic structures in a solution ANSWER: c POINTS: 1 QUESTION TYPE: Multiple Choice HAS VARIABLES: False TOPICS: Chemical Foundations of Biochemistry DATE CREATED: 11/25/2016 5:36 AM DATE MODIFIED: 12/2/2016 8:51 AM 1. What tends to happen to the percent recovery during a protein's purification? a. The number usually steadily increases during the purification. b. The number usually steadily decreases during the purification. c. The number usually stays fairly constant during the purification. d. There is no general trend for percent recovery during a protein purification. ANSWER: b POINTS: 1 QUESTION TYPE: Multiple Choice HAS VARIABLES: False LEARNING OBJECTIVES: CAFA.BIOC.15.1 - New in 6e TOPICS: Extracting Pure Proteins from Cells DATE CREATED: 12/23/2013 2:14 PM DATE MODIFIED: 12/23/2013 2:14 PM 2. Differential centrifugation at low speeds (several thousand RPM) is a useful step when a. organelles have been lysed. b. enzymes of interest have different sizes. c. cell membranes must be left intact. d. ribosomes need to be broken down. e. there are either organelles or debris to separate. ANSWER: e POINTS: 1 QUESTION TYPE: Multiple Choice HAS VARIABLES: False LEARNING OBJECTIVES: CAFA.BIOC.15.5 - Modified from 5e TOPICS: Extracting Pure Proteins from Cells DATE CREATED: 12/23/2013 2:14 PM DATE MODIFIED: 12/23/2013 2:14 PM 3. The typical order for the major steps of enzyme isolation would be (from first to last): a. Homogenization, salt fractionation, electrophoresis, column chromatography. b. Homogenization, column chromatography, salt fractionation, electrophoresis. c. Homogenization, salt fractionation, column chromatography, electrophoresis. d. Salt fractionation, homogenization, electrophoresis, column chromatography. e. Homogenization, electrophoresis, salt fractionation, column chromatography. ANSWER: c POINTS: 1 QUESTION TYPE: Multiple Choice HAS VARIABLES: False LEARNING OBJECTIVES: CAFA.BIOC.15.5 - Modified from 5e TOPICS: Extracting Pure Proteins from Cells DATE CREATED: 12/23/2013 2:14 PM DATE MODIFIED: 12/23/2013 2:14 PM 4. The purity of an enzyme at various stages of purification is best measured by a. total protein. b. total enzyme activity. c. specific activity of the enzyme. d. percent recovery of the protein. e. percent recovery of the enzyme. ANSWER: c POINTS: 1 QUESTION TYPE: Multiple Choice HAS VARIABLES: False TOPICS: Extracting Pure Proteins from Cells DATE CREATED: 12/23/2013 2:14 PM DATE MODIFIED: 12/23/2013 2:14 PM 5. Salting out with ammonium sulfate is based upon proteins interacting with other proteins via a. hydrogen bonds. b. ionic bonds. c. hydrophobic interactions. d. disulfide bonds. ANSWER: c POINTS: 1 QUESTION TYPE: Multiple Choice HAS VARIABLES: False TOPICS: Extracting Pure Proteins from Cells DATE CREATED: 12/23/2013 2:14 PM DATE MODIFIED: 12/23/2013 2:14 PM 6. Using differential centrifugation it is possible to separate a. nuclei, mitochondria, and ribosomes into three separate fractions b. organelles from contaminating salts c. proteins that differ in charge d. proteins from membranes ANSWER: a POINTS: 1 QUESTION TYPE: Multiple Choice HAS VARIABLES: False TOPICS: Extracting Pure Proteins from Cells DATE CREATED: 12/23/2013 2:14 PM DATE MODIFIED: 12/23/2013 2:14 PM 7. The following methods are useful for cell homogenization: a. Sonication. b. Freezing and thawing. c. Detergents. d. Enzymes. e. All of these are correct. ANSWER: e POINTS: 1 QUESTION TYPE: Multiple Choice HAS VARIABLES: False TOPICS: Extracting Pure Proteins from Cells DATE CREATED: 12/23/2013 2:14 PM DATE MODIFIED: 12/23/2013 2:14 PM 8. Which of the following happens as a protein is purified? a. the percent recovery and the fold purification both increase b. the percent recovery and the fold purification both decrease c. the percent recovery increases and the fold purification decreases d. the percent recovery decreases and the fold purification increases ANSWER: d POINTS: 1 QUESTION TYPE: Multiple Choice HAS VARIABLES: False TOPICS: Extracting Pure Proteins from Cells DATE CREATED: 12/23/2013 2:14 PM DATE MODIFIED: 12/23/2013 2:14 PM 9. Ammonium sulfate is useful in protein purification because a. it contains nitrogen and sulfur, both of which occur in proteins b. it is sparingly soluble in water, causing proteins to co-precipitate with it c. very pure proteins are obtained when it is used d. it forms ion-dipole interactions with water, making proteins less soluble and more likely to precipitate ANSWER: d POINTS: 1 QUESTION TYPE: Multiple Choice HAS VARIABLES: False TOPICS: Extracting Pure Proteins from Cells DATE CREATED: 12/23/2013 2:14 PM DATE MODIFIED: 12/23/2013 2:14 PM 10. The typical order of differential centrifugation for organelles is (from slowest speed/lowest g to fastest speed/highest g): a. nuclei, microsomes, mitochondria & chloroplasts, cytosol, whole cells b. whole cells, nuclei, mitochondria & chloroplasts, microsomes, cytosol c. cytosol, microsomes, nuclei, mitochondria & chloroplasts, whole cells d. nuclei, mitochondria & chloroplasts, whole cells, cytosol, microsomes e. whole cells, cytosol, microsomes, nuclei, mitochondria & chloroplasts ANSWER: b POINTS: 1 QUESTION TYPE: Multiple Choice HAS VARIABLES: False LEARNING OBJECTIVES: CAFA.BIOC.15.5 - Modified from 5e TOPICS: Extracting Pure Proteins from Cells DATE CREATED: 12/23/2013 2:14 PM DATE MODIFIED: 12/23/2013 2:14 PM 11. Which of the following are principles on which to base column chromatography? a. Molecular size b. Isoionic pH or pI c. Ion exchange d. Both molecular size and ion exchange e. All of these ANSWER: e POINTS: 1 QUESTION TYPE: Multiple Choice HAS VARIABLES: False LEARNING OBJECTIVES: CAFA.BIOC.15.8 - Modified from 6e TOPICS: Column Chromatography DATE CREATED: 12/23/2013 2:14 PM DATE MODIFIED: 12/23/2013 2:14 PM 12. Which separates on the basis of molecular weight? a. Gel filtration b. Affinity chromatography c. Cation exchange d. Anion exchange e. Cation or anion exchange ANSWER: a POINTS: 1 QUESTION TYPE: Multiple Choice HAS VARIABLES: False TOPICS: Column Chromatography DATE CREATED: 12/23/2013 2:14 PM DATE MODIFIED: 12/23/2013 2:14 PM 13. Which separates based on the ionic charge on a protein? a. Gel filtration b. Affinity chromatography c. Cation exchange d. Anion exchange e. Cation or anion exchange ANSWER: e POINTS: 1 QUESTION TYPE: Multiple Choice HAS VARIABLES: False TOPICS: Column Chromatography DATE CREATED: 12/23/2013 2:14 PM DATE MODIFIED: 12/23/2013 2:14 PM 14. Which type of column is most affected by the shape of the protein, for example, comparing spherical and cigar-shaped proteins? a. Gel filtration b. Affinity chromatography c. Cation exchange d. Anion exchange e. Cation or anion exchange ANSWER: a POINTS: 1 QUESTION TYPE: Multiple Choice HAS VARIABLES: False TOPICS: Column Chromatography DATE CREATED: 12/23/2013 2:14 PM DATE MODIFIED: 12/23/2013 2:14 PM 15. Which would be best to separate a protein that binds strongly to its substrate? a. Gel filtration b. Affinity chromatography c. Cation exchange d. Anion exchange e. Cation or anion exchange ANSWER: b POINTS: 1 QUESTION TYPE: Multiple Choice HAS VARIABLES: False TOPICS: Column Chromatography DATE CREATED: 12/23/2013 2:14 PM DATE MODIFIED: 12/23/2013 2:14 PM 16. Elution of proteins by means of a pH gradient would work best with this type of column: a. Gel filtration b. Affinity chromatography c. Cation exchange d. Anion exchange e. Cation or anion exchange ANSWER: e POINTS: 1 QUESTION TYPE: Multiple Choice HAS VARIABLES: False TOPICS: Column Chromatography DATE CREATED: 12/23/2013 2:14 PM DATE MODIFIED: 12/23/2013 2:14 PM 17. Which would be best to separate positively charged proteins? a. Gel filtration b. Affinity chromatography c. Cation exchange d. Anion exchange e. Cation or anion exchange ANSWER: c POINTS: 1 QUESTION TYPE: Multiple Choice HAS VARIABLES: False TOPICS: Column Chromatography DATE CREATED: 12/23/2013 2:14 PM DATE MODIFIED: 12/23/2013 2:14 PM 18. Which would be best to separate proteins of similar size? a. Gel filtration b. Affinity chromatography c. Cation exchange d. Anion exchange e. Cation or anion exchange ANSWER: e POINTS: 1 QUESTION TYPE: Multiple Choice HAS VARIABLES: False LEARNING OBJECTIVES: CAFA.BIOC.15.5 - Modified from 5e TOPICS: Column Chromatography DATE CREATED: 12/23/2013 2:14 PM DATE MODIFIED: 12/23/2013 2:14 PM 19. In affinity chromatography, a protein a. which binds to the ligand will remain on the column. b. which binds to the ligand will elute from the column. c. which is hydrophobic will remain on the column. d. which is hydrophilic will remain on the column. ANSWER: a POINTS: 1 QUESTION TYPE: Multiple Choice HAS VARIABLES: False TOPICS: Column Chromatography DATE CREATED: 12/23/2013 2:14 PM DATE MODIFIED: 12/23/2013 2:14 PM 20. In gel filtration chromatography a. materials are separated based on their size, the smaller ones eluting first. b. materials are separated based on their size, the larger ones eluting first. c. materials are separated based on their hydrophobic nature, the more hydrophobic ones eluting first. d. materials are separated based on their hydrophobic nature, the less hydrophobic ones eluting first. ANSWER: b POINTS: 1 QUESTION TYPE: Multiple Choice HAS VARIABLES: False TOPICS: Column Chromatography DATE CREATED: 12/23/2013 2:14 PM DATE MODIFIED: 12/23/2013 2:14 PM 21. The order of elution of AAs H, E, & K from a cation exchange column by a pH 6 buffer is a. H E K b. E H K c. K H E d. E K H ANSWER: b POINTS: 1 QUESTION TYPE: Multiple Choice HAS VARIABLES: False TOPICS: Column Chromatography DATE CREATED: 12/23/2013 2:14 PM DATE MODIFIED: 12/23/2013 2:14 PM 22. In any form of chromatography, how will a compound which interacts more strongly with the stationary phase elute compared to one that interacts less strongly? a. A compound interacting more strongly will elute earlier than one with weaker interactions. b. A compound interacting more strongly will elute later than one with weaker interactions. c. The order of elution has nothing to do with interactions with the stationary phase, but with interactions with the mobile phase. ANSWER: b POINTS: 1 QUESTION TYPE: Multiple Choice HAS VARIABLES: False LEARNING OBJECTIVES: CAFA.BIOC.15.1 - New in 6e TOPICS: Column Chromatography DATE CREATED: 12/23/2013 2:14 PM DATE MODIFIED: 12/23/2013 2:14 PM 23. A chromatography technique where a solution of nonpolar compounds is put through a column that has a nonpolar liquid immobilized on an inert matrix is which type of chromatography? a. Gel filtration b. Ion exchange c. Affinity d. HPLC e. Reverse Phase HPLC ANSWER: e POINTS: 1 QUESTION TYPE: Multiple Choice HAS VARIABLES: False LEARNING OBJECTIVES: CAFA.BIOC.15.4 - New in 7e TOPICS: Column Chromatography DATE CREATED: 12/23/2013 2:14 PM DATE MODIFIED: 12/23/2013 2:14 PM 24. In a sample consisting of lysine, leucine, and glutamic acid, which will be eluted last from an anion exchange resin at pH 7? a. all three will be eluted at the same time b. lysine c. leucine d. glutamic acid ANSWER: d POINTS: 1 QUESTION TYPE: Multiple Choice HAS VARIABLES: False LEARNING OBJECTIVES: CAFA.BIOC.15.5 - Modified from 5e TOPICS: Column Chromatography DATE CREATED: 12/23/2013 2:14 PM DATE MODIFIED: 12/23/2013 2:14 PM 25. In chromatography the experimental setup always requires a. a stationary phase and a mobile phase b. a spectrophotometric detecting device c. a sample in which components differ in charge d. a sample in which components differ in polarity ANSWER: a POINTS: 1 QUESTION TYPE: Multiple Choice HAS VARIABLES: False TOPICS: Column Chromatography DATE CREATED: 12/23/2013 2:14 PM DATE MODIFIED: 12/23/2013 2:14 PM 26. A separation of a mixture of cations of different charge requires a. another cationic substance b. an anionic substance c. an electrically neutral, but highly polar, substance d. an electrically neutral, nonpolar substance ANSWER: b POINTS: 1 QUESTION TYPE: Multiple Choice HAS VARIABLES: False TOPICS: Column Chromatography DATE CREATED: 12/23/2013 2:14 PM DATE MODIFIED: 12/23/2013 2:14 PM 27. Which of the following is not an example of column chromatography? a. ammonium sulfate fractionation b. ion-exchange separation c. HPLC d. affinity separation ANSWER: a POINTS: 1 QUESTION TYPE: Multiple Choice HAS VARIABLES: False TOPICS: Column Chromatography DATE CREATED: 12/23/2013 2:14 PM DATE MODIFIED: 12/23/2013 2:14 PM 28. In affinity chromatography a. there is nonspecific binding of proteins to column material b. only minor purifications can be obtained c. the mobile phase is always pure water d. the ligand is always specific for one type of protein to be bound e. there can be molecule specific ligands or group specific ligands ANSWER: e POINTS: 1 QUESTION TYPE: Multiple Choice HAS VARIABLES: False LEARNING OBJECTIVES: CAFA.BIOC.15.8 - Modified from 6e TOPICS: Column Chromatography DATE CREATED: 12/23/2013 2:14 PM DATE MODIFIED: 11/16/2016 4:29 AM 29. The degree of separation in molecular sieve chromatography depends on a. the polarity of the mobile phase b. the pKa of the buffer material in the mobile phase c. the chemical nature of the sieve material d. the size of the pores in the sieve material ANSWER: d POINTS: 1 QUESTION TYPE: Multiple Choice HAS VARIABLES: False TOPICS: Column Chromatography DATE CREATED: 12/23/2013 2:14 PM DATE MODIFIED: 12/23/2013 2:14 PM 30. In all forms of chromatography one way of identifying eluted substances is by a. fluorescence spectroscopy b. comparison with standards c. radioactive labeling d. treating fractions with a reagent that will cause a color change ANSWER: b POINTS: 1 QUESTION TYPE: Multiple Choice HAS VARIABLES: False TOPICS: Column Chromatography DATE CREATED: 12/23/2013 2:14 PM DATE MODIFIED: 12/23/2013 2:14 PM 31. Which substance would you expect to be eluted first from a molecular sieve column with a suitable degree of crosslinking? a. hemoglobin b. myoglobin c. 2,3-bisphosphoglycerate d. all would elute at the same rate ANSWER: a POINTS: 1 QUESTION TYPE: Multiple Choice HAS VARIABLES: False TOPICS: Column Chromatography DATE CREATED: 12/23/2013 2:14 PM DATE MODIFIED: 12/23/2013 2:14 PM 32. In the SDS-PAGE (sodium dodecylsulfate - polyacrylamide gel electrophoresis) method, separation takes place on the basis of a. charge only, because all particles have different charges, but the same mass. b. the sieving action of the gel, because all particles have the same charge, but different masses. c. the sieving action of the gel, because all particles have approximately the same charge/mass ratio, but different masses. d. the chemical nature of the buffer used as the electrolyte. ANSWER: c POINTS: 1 QUESTION TYPE: Multiple Choice HAS VARIABLES: False LEARNING OBJECTIVES: CAFA.BIOC.15.5 - Modified from 5e TOPICS: Electrophoresis DATE CREATED: 12/23/2013 2:14 PM DATE MODIFIED: 12/23/2013 2:14 PM 33. How many bands would be produced when hemoglobin is subjected to SDS-PAGE? a. 1 b. 2 c. 3 d. 4 e. 2, but only if the size of the pores in the gel would allow two proteins of slightly different size to be separated ANSWER: e POINTS: 1 QUESTION TYPE: Multiple Choice HAS VARIABLES: False LEARNING OBJECTIVES: CAFA.BIOC.15.6 - Modified in 7e TOPICS: Electrophoresis DATE CREATED: 12/23/2013 2:14 PM DATE MODIFIED: 12/23/2013 2:14 PM 34. The following are all principles on which to base electrophoresis except: a. Molecular size b. Isoionic pH or pI c. Net charge d. Binding to a substrate e. Shape ANSWER: d POINTS: 1 QUESTION TYPE: Multiple Choice HAS VARIABLES: False TOPICS: Electrophoresis DATE CREATED: 12/23/2013 2:14 PM DATE MODIFIED: 12/23/2013 2:14 PM 35. Two-dimensional electrophoresis usually exploits these 2 different properties of proteins: a. Molecular weight and shape b. Molecular weight and net charge c. Molecular weight and isoionic pH d. Isoionic pH and shape e. Isoionic pH and net charge ANSWER: b POINTS: 1 QUESTION TYPE: Multiple Choice HAS VARIABLES: False TOPICS: Electrophoresis DATE CREATED: 12/23/2013 2:14 PM DATE MODIFIED: 12/23/2013 2:14 PM Exhibit 5A The following diagram shows the anode, cathode, and pH gradient of an isoelectric focusing bed: anode+ −cathode pH 1 2 3 4 5 6 7 8 9 10 11 12 13 14 36. Refer to Exhibit 5A. If the amino acid glycine were placed in the bed where the pH is 7, and the current were turned on, it would migrate closest to which of the following positions? a. pH 4 b. pH 6 c. pH 8 d. pH 10 ANSWER: b POINTS: 1 QUESTION TYPE: Multiple Choice HAS VARIABLES: False PREFACE NAME: Exhibit 5A LEARNING OBJECTIVES: CAFA.BIOC.15.5 - Modified from 5e TOPICS: Electrophoresis DATE CREATED: 12/23/2013 2:14 PM DATE MODIFIED: 12/23/2013 2:14 PM 37. Refer to Exhibit 5A. If the amino acid glycine were placed in the bed where the pH is 11, and the current were turned on, it would migrate closest to which of the following positions? a. pH 4 b. pH 6 c. pH 8 d. pH 10 ANSWER: b POINTS: 1 QUESTION TYPE: Multiple Choice HAS VARIABLES: False PREFACE NAME: Exhibit 5A LEARNING OBJECTIVES: CAFA.BIOC.15.5 - Modified from 5e TOPICS: Electrophoresis DATE CREATED: 12/23/2013 2:14 PM DATE MODIFIED: 12/23/2013 2:14 PM 38. Refer to Exhibit 5A. A mixture of asp, asn, and arg is placed in the bed where the pH is 7, and the current is turned on. From left to right, which best represents the final positions of the individual amino acids? a. asp asn arg b. arg asn asp c. asn asp arg d. arg asp asn ANSWER: a POINTS: 1 QUESTION TYPE: Multiple Choice HAS VARIABLES: False PREFACE NAME: Exhibit 5A LEARNING OBJECTIVES: CAFA.BIOC.15.5 - Modified from 5e TOPICS: Electrophoresis DATE CREATED: 12/23/2013 2:14 PM DATE MODIFIED: 12/23/2013 2:14 PM 39. When electrophoretic separations are done based on molecular weight, the distance that a molecule moves can be graphed as a straight line when compared to: a. the MW of the proteins b. the negative of the MW of the proteins c. the log of the MW of the proteins d. none of these ANSWER: c POINTS: 1 QUESTION TYPE: Multiple Choice HAS VARIABLES: False LEARNING OBJECTIVES: CAFA.BIOC.15.4 - New in 7e TOPICS: Electrophoresis DATE CREATED: 12/23/2013 2:14 PM DATE MODIFIED: 12/23/2013 2:14 PM 40. In electrophoresis experiments a. the separation must be carried out in bright light b. the polarity of substances to be separated is more important than their charge or size c. the sample can be badly degraded as a result of the separation d. an electric field must be applied to the mixture to be separated ANSWER: d POINTS: 1 QUESTION TYPE: Multiple Choice HAS VARIABLES: False TOPICS: Electrophoresis DATE CREATED: 12/23/2013 2:14 PM DATE MODIFIED: 12/23/2013 2:14 PM 41. In isoelectric focusing gel electrophoresis a. particular care must be taken to ensure the same pH along the length of the gel b. there is a pH gradient that parallels the electric field gradient c. the electric current is allowed to fluctuate d. the electric circuits of the apparatus must be very well insulated ANSWER: b POINTS: 1 QUESTION TYPE: Multiple Choice HAS VARIABLES: False TOPICS: Electrophoresis DATE CREATED: 12/23/2013 2:14 PM DATE MODIFIED: 12/23/2013 2:14 PM 42. The isoelectric point is a. the pH at which a substance has no net charge b. the pH at which a substance has a net positive charge c. the pH at which a substance has a net negative charge d. the pH at which a substance has no charge groups of any kind ANSWER: a POINTS: 1 QUESTION TYPE: Multiple Choice HAS VARIABLES: False TOPICS: Electrophoresis DATE CREATED: 12/23/2013 2:14 PM DATE MODIFIED: 12/23/2013 2:14 PM 43. Two dimensional separation methods a. lead to unreliable results b. are not widely used because of their complexity c. do not improve separation d. consist of two separation methods applied in sequence ANSWER: d POINTS: 1 QUESTION TYPE: Multiple Choice HAS VARIABLES: False TOPICS: Electrophoresis DATE CREATED: 12/23/2013 2:14 PM DATE MODIFIED: 12/23/2013 2:14 PM 44. Important steps in sequencing pure proteins include all of these except: a. Determining the amino acid composition b. Determining the isoionic pH of the protein c. Breaking the protein into smaller peptides d. Determining the amino acids on the ends of the protein e. Determining the amino acids on the ends of the smaller peptides ANSWER: b POINTS: 1 QUESTION TYPE: Multiple Choice HAS VARIABLES: False TOPICS: Primary Structure Determination DATE CREATED: 12/23/2013 2:14 PM DATE MODIFIED: 12/23/2013 2:14 PM 45. If a protein with the sequence FEWPRQVDMARINE is treated with chymotrypsin, what will the products be? a. F EW PRQVMARINE b. FE WPRQVD MARINE c. FEWPR QVDMAR INE d. FEWPRQVDM ARINE ANSWER: a POINTS: 1 QUESTION TYPE: Multiple Choice HAS VARIABLES: False LEARNING OBJECTIVES: CAFA.BIOC.15.1 - New in 6e TOPICS: Primary Structure Determination DATE CREATED: 12/23/2013 2:14 PM DATE MODIFIED: 12/23/2013 2:14 PM 46. Which of the following treatments results in a polypeptide fragment with a homoserine lactone at the C-terminal end? a. trypsin b. chymotrypsin c. cyanogen bromide d. Edman method ANSWER: c POINTS: 1 QUESTION TYPE: Multiple Choice HAS VARIABLES: False LEARNING OBJECTIVES: CAFA.BIOC.15.1 - New in 6e TOPICS: Primary Structure Determination DATE CREATED: 12/23/2013 2:14 PM DATE MODIFIED: 12/23/2013 2:14 PM 47. If a protein with the sequence PQRKYPIG is treated with trypsin, what will the products be? a. PQR KYPIG b. PQRK YPIG c. PQR K YPIG d. PQ R KPIG0 ANSWER: c POINTS: 1 QUESTION TYPE: Multiple Choice HAS VARIABLES: False TOPICS: Primary Structure Determination DATE CREATED: 12/23/2013 2:14 PM DATE MODIFIED: 12/23/2013 2:14 PM 48. Cyanogen bromide (CNBr) cleaves proteins a. after positively charged residues, such as K & R. b. after negatively charged residues, such as D & E. c. after aromatic residues, such as Y & W. d. after methionine residues. ANSWER: d POINTS: 1 QUESTION TYPE: Multiple Choice HAS VARIABLES: False TOPICS: Primary Structure Determination DATE CREATED: 12/23/2013 2:14 PM DATE MODIFIED: 12/23/2013 2:14 PM 49. It is impossible to sequence a protein if you do not have overlapping sequences to work with. a. True b. False ANSWER: b POINTS: 1 QUESTION TYPE: Multiple Choice HAS VARIABLES: False TOPICS: Primary Structure Determination DATE CREATED: 12/23/2013 2:14 PM DATE MODIFIED: 12/23/2013 2:14 PM 50. The most efficient method for determining the sequence of a short peptide is: a. Edman degradation b. Trypsin digestion c. Chymotrypsin digestion d. Cyanogen bromide digestion ANSWER: a POINTS: 1 QUESTION TYPE: Multiple Choice HAS VARIABLES: False LEARNING OBJECTIVES: CAFA.BIOC.15.6 - Modified in 7e TOPICS: Primary Structure Determination DATE CREATED: 12/23/2013 2:14 PM DATE MODIFIED: 12/23/2013 2:14 PM 51. Methods for breaking proteins into smaller peptides include all of the following except: a. Digestion with chymotrypsin b. Cyanogen Bromide treatment c. Digestion with Trypsin d. Edmann degradation e. All of the above create short peptides suitable for sequencing ANSWER: d POINTS: 1 QUESTION TYPE: Multiple Choice HAS VARIABLES: False TOPICS: Primary Structure Determination DATE CREATED: 12/23/2013 2:14 PM DATE MODIFIED: 12/23/2013 2:14 PM 52. When end-group analysis was done on the protein insulin, the results indicated that both glycine and phenylalanine were N-terminal amino acids and both asparagine and alanine were C-terminal amino acids. These results indicate that a. the experiment was done incorrectly b. no conclusions can be drawn c. there were impurities in the sample d. insulin consists of two polypeptide chains ANSWER: d POINTS: 1 QUESTION TYPE: Multiple Choice HAS VARIABLES: False TOPICS: Primary Structure Determination DATE CREATED: 12/23/2013 2:14 PM DATE MODIFIED: 12/23/2013 2:14 PM 53. Matrix-Assisted Laser Desorption Ionization is a type of _____ technique. a. electrophoresis b. ion exchange chromatography c. affinity chromatography d. mass spectrometry ANSWER: d POINTS: 1 QUESTION TYPE: Multiple Choice HAS VARIABLES: False LEARNING OBJECTIVES: CAFA.BIOC.15.4 - New in 7e TOPICS: Primary Structure Determination DATE CREATED: 12/23/2013 2:14 PM DATE MODIFIED: 11/29/2016 6:53 AM 54. An amino acid analyzer is an instrument used to determine a. the sequence of amino acids in a polypeptide chain b. the identity of N-terminal and C-terminal amino acids in a protein c. the presence of modified amino acids in a protein d. the identities and relative amounts of amino acids in a protein ANSWER: d POINTS: 1 QUESTION TYPE: Multiple Choice HAS VARIABLES: False TOPICS: Primary Structure Determination DATE CREATED: 12/23/2013 2:14 PM DATE MODIFIED: 12/23/2013 2:14 PM 55. Generally speaking, sequence techniques have become so sensitive that if you are able to isolate the protein on a gel, there is enough of it to get a significant amount of its sequence. a. True b. False ANSWER: a POINTS: 1 QUESTION TYPE: Multiple Choice HAS VARIABLES: False TOPICS: Primary Structure Determination DATE CREATED: 12/23/2013 2:14 PM DATE MODIFIED: 12/23/2013 2:14 PM 56. Determination of the sequence of amino acids in a peptide is done by a. x-ray crystallography b. Edman degradation c. treatment with cyanogen bromide d. trypsin hydrolysis ANSWER: b POINTS: 1 QUESTION TYPE: Multiple Choice HAS VARIABLES: False TOPICS: Primary Structure Determination DATE CREATED: 12/23/2013 2:14 PM DATE MODIFIED: 12/23/2013 2:14 PM 57. It is frequently possible to bypass the determination of the identity of the N-terminal amino acid of a protein because a. this information is already available from the amino acid analysis b. the Edman method sequences the peptide from the N-terminal end c. N-terminal amino acids are always chemically modified d. this information is not needed ANSWER: b POINTS: 1 QUESTION TYPE: Multiple Choice HAS VARIABLES: False TOPICS: Primary Structure Determination DATE CREATED: 12/23/2013 2:14 PM DATE MODIFIED: 12/23/2013 2:14 PM 58. Which of the following is not used in protein structure determination? a. digestion with proteolytic enzymes b. the Edman method c. treatment with cyanogen bromide d. treatment with alkyl halides ANSWER: d POINTS: 1 QUESTION TYPE: Multiple Choice HAS VARIABLES: False TOPICS: Primary Structure Determination DATE CREATED: 12/23/2013 2:14 PM DATE MODIFIED: 12/23/2013 2:14 PM 59. In an ELISA, the molecule that binds directly to the target protein is called the a. primary antibody b. secondary antibody c. linked enzyme d. immunosorbent ANSWER: a POINTS: 1 QUESTION TYPE: Multiple Choice HAS VARIABLES: False LEARNING OBJECTIVES: CAFA.BIOC.15.3 - New in 8e TOPICS: Protein identification techniques DATE CREATED: 12/23/2013 2:14 PM DATE MODIFIED: 12/23/2013 2:14 PM 60. Which of the following explains why a secondary antibody is used in an ELISA? a. two antibodies will be twice as visible as one antibody b. it allows the visible signal to be amplified c. primary antibodies cannot be tagged with visible markers d. none of the choices ANSWER: b POINTS: 1 QUESTION TYPE: Multiple Choice HAS VARIABLES: False LEARNING OBJECTIVES: CAFA.BIOC.15.3 - New in 8e TOPICS: Protein identification techniques DATE CREATED: 12/23/2013 2:14 PM DATE MODIFIED: 12/23/2013 2:14 PM 61. In an ELISA, the compound 4-chloro-1-naphthol is used because a. it helps the secondary antibody to bind to the protein b. it helps the primary antibody bind to the protein c. it turns color in the presence of an enzyme that is bound to the secondary antibody d. all of the choices ANSWER: c POINTS: 1 QUESTION TYPE: Multiple Choice HAS VARIABLES: False LEARNING OBJECTIVES: CAFA.BIOC.15.3 - New in 8e TOPICS: Protein identification techniques DATE CREATED: 12/23/2013 2:14 PM DATE MODIFIED: 12/23/2013 2:14 PM 62. Which of the following is NOT true concerning a western blot? a. it is usually performed in a 96 well microtiter plate b. it involves the use of primary antibodies c. it involves the use of secondary antibodies d. it involves electrophoresis ANSWER: a POINTS: 1 QUESTION TYPE: Multiple Choice HAS VARIABLES: False LEARNING OBJECTIVES: CAFA.BIOC.15.3 - New in 8e TOPICS: Protein identification techniques DATE CREATED: 12/23/2013 2:14 PM DATE MODIFIED: 12/23/2013 2:14 PM 63. The purpose of using nitrocellulose for a western blot is to a. allow the various proteins to migrate and separate b. bind to the primary antibody c. allow the proteins to be concentrated onto the face of the nitrocellulose where they will be accessible to the primary antibody d. to allow the 4-chloro-1-naphthol to react and be visible ANSWER: c POINTS: 1 QUESTION TYPE: Multiple Choice HAS VARIABLES: False LEARNING OBJECTIVES: CAFA.BIOC.15.3 - New in 8e TOPICS: Protein identification techniques DATE CREATED: 12/23/2013 2:14 PM DATE MODIFIED: 12/23/2013 2:14 PM 64. A protein chip is functionally most similar to which other technique? a. gel filtration b. ELISA c. SDS-PAGE d. western blot ANSWER: b POINTS: 1 QUESTION TYPE: Multiple Choice HAS VARIABLES: False LEARNING OBJECTIVES: CAFA.BIOC.15.3 - New in 8e TOPICS: Protein identification techniques DATE CREATED: 12/23/2013 2:14 PM DATE MODIFIED: 12/23/2013 2:14 PM 65. The most common way of visualizing the results seen on a protein chip is by use of a. secondary antibodies b. primary antibodies c. fluorescence d. using an enzyme-linked reaction involving 4-chloro-1-naphthol ANSWER: c POINTS: 1 QUESTION TYPE: Multiple Choice HAS VARIABLES: False LEARNING OBJECTIVES: CAFA.BIOC.15.3 - New in 8e TOPICS: Protein identification techniques DATE CREATED: 12/23/2013 2:14 PM DATE MODIFIED: 12/23/2013 2:14 PM 66. Studying the entire complement